Gene description for NFKB1
Gene name nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Gene symbol NFKB1
Other names/aliases EBP-1
KBF1
NF-kB1
NF-kappa-B
NF-kappaB
NFKB-p105
NFKB-p50
NFkappaB
p105
p50
Species Homo sapiens
 Database cross references - NFKB1
ExoCarta ExoCarta_4790
Vesiclepedia VP_4790
Entrez Gene 4790
HGNC 7794
MIM 164011
UniProt P19838  
 NFKB1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NFKB1
Molecular Function
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001205 IDA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0001227 IDA
    chromatin binding GO:0003682 IBA
    protein heterodimerization activity GO:0046982 IDA
    protein binding GO:0005515 IPI
    transcription regulatory region DNA binding GO:0044212 IDA
    regulatory region DNA binding GO:0000975 IDA
    transcription regulatory region sequence-specific DNA binding GO:0000976 IDA
    protein homodimerization activity GO:0042803 IEA
    transcription factor binding GO:0008134 IDA
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IDA
    heat shock protein binding GO:0031072 IEA
    identical protein binding GO:0042802 IPI
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    double-stranded DNA binding GO:0003690 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
Biological Process
    response to oxidative stress GO:0006979 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    cellular response to interleukin-6 GO:0071354 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    cellular response to lipopolysaccharide GO:0071222 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    positive regulation of lipid storage GO:0010884 IC
    negative regulation of interleukin-12 biosynthetic process GO:0045083 IEA
    membrane protein intracellular domain proteolysis GO:0031293 TAS
    cellular response to peptide hormone stimulus GO:0071375 IMP
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    inflammatory response GO:0006954 TAS
    negative regulation of cellular protein metabolic process GO:0032269 IC
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of type I interferon production GO:0032481 TAS
    cellular response to mechanical stimulus GO:0071260 IEP
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    NIK/NF-kappaB signaling GO:0038061 IBA
    negative regulation of cholesterol transport GO:0032375 IC
    positive regulation of hyaluronan biosynthetic process GO:1900127 IDA
    apoptotic process GO:0006915 IEA
    innate immune response GO:0045087 TAS
    negative regulation of inflammatory response GO:0050728 IEA
    positive regulation of miRNA metabolic process GO:2000630 IMP
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IC
    negative regulation of vitamin D biosynthetic process GO:0010957 IC
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IBA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    cellular response to nicotine GO:0071316 IMP
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    negative regulation of calcidiol 1-monooxygenase activity GO:0010956 IDA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    response to copper ion GO:0046688 IEA
    cellular response to interleukin-1 GO:0071347 IEP
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    I-kappaB/NF-kappaB complex GO:0033256 TAS
    mitochondrion GO:0005739 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified NFKB1 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
ISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NFKB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 G3BP2 9908
Invivo Homo sapiens
Invitro Homo sapiens
2 RELA 5970
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 NCOA6  
Reconstituted Complex Homo sapiens
4 NKRF 55922
Invitro Homo sapiens
5 PPP4C 5531
Invivo Homo sapiens
Invitro Homo sapiens
6 KPNA3 3839
Affinity Capture-MS Homo sapiens
7 PARP1 142
Invivo Homo sapiens
Invitro Homo sapiens
8 ELF1 1997
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 HBXAP  
Affinity Capture-Western Homo sapiens
10 GSK3B 2932
Biochemical Activity Homo sapiens
11 MTPN 136319
Invitro Homo sapiens
12 TP53BP1 7158
Affinity Capture-Western Homo sapiens
13 NFKBIZ  
Invivo Homo sapiens
14 LYL1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 NCOA1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 SP1  
Reconstituted Complex Homo sapiens
17 REL 5966
Affinity Capture-MS Homo sapiens
18 ELF3 1999
Reconstituted Complex Homo sapiens
19 NFKBIA  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
20 MEN1 4221
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 MAP3K7IP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 IKBKG 8517
Affinity Capture-MS Homo sapiens
23 IKBKB 3551
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
24 NR3C1 2908
Reconstituted Complex Homo sapiens
25 NFKBIE  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
26 HMGB1 3146
Reconstituted Complex Homo sapiens
27 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 BCL3  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 MAP3K8  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
30 NCOR2  
Invitro Homo sapiens
31 NCF1  
Invitro Homo sapiens
Invivo Homo sapiens
32 TNIP2  
Affinity Capture-MS Homo sapiens
33 BTRC 8945
Affinity Capture-Western Homo sapiens
34 ITGB3BP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
35 TBK1 29110
Affinity Capture-MS Homo sapiens
36 TXN 7295
Invitro Homo sapiens
37 NCOR1  
Affinity Capture-Western Homo sapiens
38 IRF2 3660
Invitro Homo sapiens
39 IL2RA  
Invivo Homo sapiens
Invitro Homo sapiens
40 RXRA 6256
Reconstituted Complex Homo sapiens
41 TRIP4 9325
Invitro Homo sapiens
42 KLF5  
Affinity Capture-Western Homo sapiens
43 STAT3 6774
Invivo Homo sapiens
Invitro Homo sapiens
44 RELB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 COPB2 9276
Affinity Capture-MS Homo sapiens
46 SPAG9 9043
Affinity Capture-MS Homo sapiens
47 NFKB1 4790
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
48 CEBPB  
Invivo Homo sapiens
Invitro Homo sapiens
49 E2F1 1869
Invitro Homo sapiens
Invivo Homo sapiens
50 STAT6 6778
Invivo Homo sapiens
Invitro Homo sapiens
51 UNC5CL  
Affinity Capture-Western Homo sapiens
52 TNIP3  
Affinity Capture-MS Homo sapiens
53 NFKB2 4791
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TSC22D3  
Invitro Homo sapiens
Invivo Homo sapiens
55 CHUK 1147
Affinity Capture-Western Homo sapiens
56 NOTCH1 4851
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 HMGA2 8091
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
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