Gene description for NFYC
Gene name nuclear transcription factor Y, gamma
Gene symbol NFYC
Other names/aliases CBF-C
CBFC
H1TF2A
HAP5
HSM
NF-YC
Species Homo sapiens
 Database cross references - NFYC
ExoCarta ExoCarta_4802
Vesiclepedia VP_4802
Entrez Gene 4802
HGNC 7806
MIM 605344
UniProt Q13952  
 NFYC identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NFYC
Molecular Function
    transcription coactivator activity GO:0003713 TAS
    protein binding GO:0005515 IPI
    transcription regulatory region DNA binding GO:0044212 IDA
    protein heterodimerization activity GO:0046982 IEA
    sequence-specific DNA binding GO:0043565 IEA
    transcription factor binding GO:0008134 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    DNA binding GO:0003677 IDA
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    small molecule metabolic process GO:0044281 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    regulation of transcription, DNA-templated GO:0006355 IDA
    protein folding GO:0006457 TAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    CCAAT-binding factor complex GO:0016602 IDA
    protein-DNA complex GO:0032993 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified NFYC in exosomes
1
Experiment ID 237
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NFYC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CREBL1  
Invitro Homo sapiens
Invivo Homo sapiens
2 SMAD3 4088
Affinity Capture-Western Homo sapiens
3 MYC  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 TBP  
Reconstituted Complex Homo sapiens
5 SMAD2 4087
Affinity Capture-Western Homo sapiens
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