Gene description for NFYC
Gene name nuclear transcription factor Y, gamma
Gene symbol NFYC
Other names/aliases CBF-C
CBFC
H1TF2A
HAP5
HSM
NF-YC
Species Homo sapiens
 Database cross references - NFYC
ExoCarta ExoCarta_4802
Vesiclepedia VP_4802
Entrez Gene 4802
HGNC 7806
MIM 605344
UniProt Q13952  
 NFYC identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NFYC
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    DNA binding GO:0003677 IDA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    protein folding GO:0006457 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    CCAAT-binding factor complex GO:0016602 IBA
    CCAAT-binding factor complex GO:0016602 IDA
    CCAAT-binding factor complex GO:0016602 IPI
    protein-DNA complex GO:0032993 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified NFYC in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NFYC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GLTSCR1L  
Two-hybrid Homo sapiens
2 ALMS1  
Proximity Label-MS Homo sapiens
3 SP1  
Two-hybrid Homo sapiens
4 MID1IP1  
Proximity Label-MS Homo sapiens
5 TRIM27  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
6 FKBP4 2288
Proximity Label-MS Homo sapiens
7 KANSL3  
Proximity Label-MS Homo sapiens
8 MILR1  
Affinity Capture-MS Homo sapiens
9 GLTSCR1 29998
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
10 TAF5  
Proximity Label-MS Homo sapiens
11 HDAC1 3065
Affinity Capture-Western Homo sapiens
12 CAPZB 832
Affinity Capture-MS Homo sapiens
13 CORO1B 57175
Co-fractionation Homo sapiens
14 C11orf30  
Proximity Label-MS Homo sapiens
15 VPS72  
Proximity Label-MS Homo sapiens
16 RFXANK  
Proximity Label-MS Homo sapiens
17 KRT40  
Affinity Capture-MS Homo sapiens
18 ARID4B  
Proximity Label-MS Homo sapiens
19 NPM1 4869
Co-fractionation Homo sapiens
20 STAT3 6774
Co-fractionation Homo sapiens
21 ST13P4 145165
Proximity Label-MS Homo sapiens
22 NXT1  
Affinity Capture-MS Homo sapiens
23 NR2C1  
Proximity Label-MS Homo sapiens
24 YY1AP1 55249
Proximity Label-MS Homo sapiens
25 TFCP2 7024
Proximity Label-MS Homo sapiens
26 SETD1A 9739
Proximity Label-MS Homo sapiens
27 ASNSD1  
Proximity Label-MS Homo sapiens
28 KIF3A 11127
Affinity Capture-MS Homo sapiens
29 LMO4  
Two-hybrid Homo sapiens
30 PRR20A  
Affinity Capture-MS Homo sapiens
31 RFX5 5993
Proximity Label-MS Homo sapiens
32 ZDHHC17 23390
Two-hybrid Homo sapiens
33 NFRKB  
Proximity Label-MS Homo sapiens
34 HIVEP1 3096
Proximity Label-MS Homo sapiens
35 FHL2 2274
Affinity Capture-MS Homo sapiens
36 TAF11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
37 PON1 5444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SREBF2 6721
Two-hybrid Homo sapiens
39 POGZ 23126
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
40 TTN 7273
Affinity Capture-MS Homo sapiens
41 TAF12  
Reconstituted Complex Homo sapiens
42 FOXK2  
Affinity Capture-MS Homo sapiens
43 SMAD3 4088
Affinity Capture-Western Homo sapiens
44 ZNF131  
Proximity Label-MS Homo sapiens
45 SRF  
Affinity Capture-MS Homo sapiens
46 SMAD2 4087
Affinity Capture-Western Homo sapiens
47 CIC 23152
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
48 L3MBTL2  
Proximity Label-MS Homo sapiens
49 SP2  
Proximity Label-MS Homo sapiens
50 CACYBP 27101
Proximity Label-MS Homo sapiens
51 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MTR 4548
Proximity Label-MS Homo sapiens
53 DST 667
Two-hybrid Homo sapiens
54 ASXL2  
Proximity Label-MS Homo sapiens
55 KMT2A  
Proximity Label-MS Homo sapiens
56 RFX7  
Proximity Label-MS Homo sapiens
57 ATF6  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
58 BAG2 9532
Affinity Capture-MS Homo sapiens
59 CSN2 1447
Two-hybrid Homo sapiens
60 MGA  
Proximity Label-MS Homo sapiens
61 CTNNB1 1499
Co-fractionation Homo sapiens
62 PRCP 5547
Co-fractionation Homo sapiens
63 DDX60 55601
Affinity Capture-MS Homo sapiens
64 ZNF143  
Proximity Label-MS Homo sapiens
65 Nfya  
Affinity Capture-MS Mus musculus
66 KANSL2  
Proximity Label-MS Homo sapiens
67 EP400  
Proximity Label-MS Homo sapiens
68 BAP1 8314
Proximity Label-MS Homo sapiens
69 TP73  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 TGM7  
Affinity Capture-MS Homo sapiens
71 TBP  
Reconstituted Complex Homo sapiens
72 LIN9  
Proximity Label-MS Homo sapiens
73 MYC  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
74 BAG1 573
Affinity Capture-MS Homo sapiens
75 TOP1 7150
Co-fractionation Homo sapiens
76 NFKB1 4790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CDC37 11140
Proximity Label-MS Homo sapiens
78 CR1L 1379
Affinity Capture-MS Homo sapiens
79 NFYA 4800
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 NFATC2  
Affinity Capture-MS Homo sapiens
81 CORO1C 23603
Co-fractionation Homo sapiens
82 TAF4 6874
Proximity Label-MS Homo sapiens
83 XRCC5 7520
Co-fractionation Homo sapiens
84 COMMD1 150684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 TAF6 6878
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
86 PON3 5446
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 PARP1 142
Co-fractionation Homo sapiens
88 ARID2  
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
89 JUP 3728
Co-fractionation Homo sapiens
90 Cebpb  
Affinity Capture-Western Rattus norvegicus
91 PHF12  
Proximity Label-MS Homo sapiens
92 PCMT1 5110
Proximity Label-MS Homo sapiens
93 FOXJ2  
Affinity Capture-MS Homo sapiens
94 CXXC1  
Proximity Label-MS Homo sapiens
95 KAT2B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 DR1  
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
97 PBRM1 55193
Proximity Label-MS Homo sapiens
98 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
99 GON4L  
Proximity Label-MS Homo sapiens
100 FCGR2A 2212
Affinity Capture-MS Homo sapiens
101 SSBP1 6742
Affinity Capture-MS Homo sapiens
102 ATF6B  
Reconstituted Complex Homo sapiens
103 NFYB  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 GAPDH 2597
Two-hybrid Homo sapiens
105 PRR20E  
Affinity Capture-MS Homo sapiens
106 RFXAP  
Proximity Label-MS Homo sapiens
107 LIN54  
Proximity Label-MS Homo sapiens
108 XRCC6 2547
Co-fractionation Homo sapiens
109 SNX2 6643
Two-hybrid Homo sapiens
110 KRAS 3845
Negative Genetic Homo sapiens
111 GATAD1  
Proximity Label-MS Homo sapiens
112 TBPL2  
Co-localization Homo sapiens
113 KIAA2018  
Two-hybrid Homo sapiens
114 ADNP2  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here