Gene description for NFYA
Gene name nuclear transcription factor Y, alpha
Gene symbol NFYA
Other names/aliases CBF-A
CBF-B
HAP2
NF-YA
Species Homo sapiens
 Database cross references - NFYA
ExoCarta ExoCarta_4800
Entrez Gene 4800
HGNC 7804
MIM 189903
UniProt P23511  
 NFYA identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for NFYA
Molecular Function
    transcription cofactor binding GO:0001221 IEA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    DNA binding GO:0003677 IDA
    transcription regulatory region DNA binding GO:0044212 IDA
    core promoter sequence-specific DNA binding GO:0001046 ISS
Biological Process
    small molecule metabolic process GO:0044281 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    positive regulation of stem cell proliferation GO:2000648 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    regulation of transcription, DNA-templated GO:0006355 IDA
    regulation of stem cell maintenance GO:2000036 IEA
    rhythmic process GO:0048511 IEA
    positive regulation of transcription, DNA-templated GO:0045893 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    CCAAT-binding factor complex GO:0016602 IDA
    nucleoplasm GO:0005654 TAS
    protein-DNA complex GO:0032993 IDA
 Experiment description of studies that identified NFYA in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NFYA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRF  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 APPBP2  
Two-hybrid Homo sapiens
3 JUN 3725
Invivo Homo sapiens
4 BRCA1 672
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 NFYB  
Invitro Homo sapiens
6 HMGA1 3159
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
7 POU2F1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 ZHX1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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