Experiment description of studies that identified TCAF1 in exosomes

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

8 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for TCAF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 NOTCH2NL 388677 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 2 TRIP6 7205 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 3 BIRC2   Two-hybrid Homo sapiens 4 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 5 ZNF24   Two-hybrid Homo sapiens 6 CNKSR1   Two-hybrid Homo sapiens 7 NSUN5P1   Affinity Capture-MS Homo sapiens 8 TRAF2 7186 Two-hybrid Homo sapiens 9 ZNF592 9640 Two-hybrid Homo sapiens 10 L3HYPDH 112849 Affinity Capture-MS Homo sapiens 11 SPRY2 10253 Two-hybrid Homo sapiens 12 COIL   Two-hybrid Homo sapiens 13 ATXN3 4287 Affinity Capture-MS Homo sapiens 14 PRDM14   Two-hybrid Homo sapiens 15 TP53 7157 Affinity Capture-MS Homo sapiens 16 TAX1BP1 8887 Two-hybrid Homo sapiens 17 USP13   Affinity Capture-MS Homo sapiens 18 IKZF3   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 19 DNAJA2 10294 Affinity Capture-MS Homo sapiens 20 CD2BP2 10421 Affinity Capture-MS Homo sapiens 21 SF3B4 10262 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 22 KRTAP10-8   Two-hybrid Homo sapiens 23 TOLLIP 54472 Affinity Capture-MS Homo sapiens 24 APBA3   Affinity Capture-MS Homo sapiens 25 UBA52 7311 Affinity Capture-MS Homo sapiens 26 DISC1 27185 Two-hybrid Homo sapiens 27 ZNF143   Two-hybrid Homo sapiens 28 CUL3 8452 Affinity Capture-MS Homo sapiens 29 PPARD   Affinity Capture-MS Homo sapiens 30 LYPD6B 130576 Affinity Capture-MS Homo sapiens 31 UBC 7316 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 32 RBAK   Two-hybrid Homo sapiens 33 IKZF4   Two-hybrid Homo sapiens 34 AAR2 25980 Affinity Capture-MS Homo sapiens 35 ZNF398   Affinity Capture-MS Homo sapiens 36 CTAG2   Affinity Capture-MS Homo sapiens 37 PEG10 23089 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 38 DNAJB3 414061 Affinity Capture-MS Homo sapiens 39 KRTAP13-2   Two-hybrid Homo sapiens 40 TRIM42   Two-hybrid Homo sapiens 41 LIG1 3978 Co-fractionation Homo sapiens 42 TRIM54   Two-hybrid Homo sapiens 43 PWP1 11137 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 LRP1 4035 Affinity Capture-MS Homo sapiens 45 CEP250 11190 Affinity Capture-MS Homo sapiens 46 MAPK6   Affinity Capture-MS Homo sapiens 47 DDX41 51428 Affinity Capture-MS Homo sapiens 48 GPRC5C 55890 Affinity Capture-MS Homo sapiens 49 MID2 11043 Two-hybrid Homo sapiens 50 CTAG1B   Affinity Capture-MS Homo sapiens 51 CEP70   Two-hybrid Homo sapiens 52 SREK1IP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 CYSRT1 375791 Two-hybrid Homo sapiens 54 NTRK1 4914 Affinity Capture-MS Homo sapiens 55 KRTAP1-3   Two-hybrid Homo sapiens 56 CIAPIN1 57019 Affinity Capture-MS Homo sapiens 57 GPR182   Affinity Capture-MS Homo sapiens 58 TCF3   Two-hybrid Homo sapiens 59 TNNC2   Affinity Capture-MS Homo sapiens 60 RSPH6A   Affinity Capture-MS Homo sapiens 61 SHARPIN   Affinity Capture-MS Homo sapiens 62 HUWE1 10075 Affinity Capture-MS Homo sapiens 63 FBXL6   Affinity Capture-MS Homo sapiens 64 TFIP11   Two-hybrid Homo sapiens 65 LAGE3   Affinity Capture-MS Homo sapiens 66 GCG   Affinity Capture-MS Homo sapiens 67 C9orf72   Affinity Capture-MS Homo sapiens 68 PPM1M   Affinity Capture-MS Homo sapiens 69 NFYA 4800 Two-hybrid Homo sapiens 70 NBPF19   Two-hybrid Homo sapiens 71 RPA2 6118 Affinity Capture-MS Homo sapiens 72 CLEC14A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 TGDS   Affinity Capture-MS Homo sapiens 74 GET4 51608 Affinity Capture-MS Homo sapiens 75 UBXN1 51035 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 76 KRTAP6-3   Two-hybrid Homo sapiens 77 ZNF263   Two-hybrid Homo sapiens 78 EXOSC5 56915 Affinity Capture-MS Homo sapiens 79 EFEMP2 30008 Two-hybrid Homo sapiens 80 RPA3 6119 Proximity Label-MS Homo sapiens 81 PPM1F 9647 Co-fractionation Homo sapiens View the network

Pathways in which TCAF1 is involved

No pathways found