Gene description for TRAF2
Gene name TNF receptor-associated factor 2
Gene symbol TRAF2
Other names/aliases MGC:45012
TRAP
TRAP3
Species Homo sapiens
 Database cross references - TRAF2
ExoCarta ExoCarta_7186
Vesiclepedia VP_7186
Entrez Gene 7186
HGNC 12032
MIM 601895
UniProt Q12933  
 TRAF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TRAF2
Molecular Function
    thioesterase binding GO:0031996 IPI
    signal transducer activity GO:0004871 NAS
    ligase activity GO:0016874 IEA
    enzyme binding GO:0019899 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein phosphatase binding GO:0019903 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein complex binding GO:0032403 IEA
    tumor necrosis factor receptor binding GO:0005164 IPI
    protein kinase binding GO:0019901 IPI
    identical protein binding GO:0042802 IPI
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IEA
    sphingolipid binding GO:0046625 IDA
    protein binding GO:0005515 IPI
    CD40 receptor binding GO:0005174 ISS
Biological Process
    innate immune response GO:0045087 TAS
    negative regulation of glial cell apoptotic process GO:0034351 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 TAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 IMP
    positive regulation of interleukin-2 production GO:0032743 IMP
    positive regulation of T cell cytokine production GO:0002726 IMP
    protein heterooligomerization GO:0051291 IEA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    signal transduction GO:0007165 TAS
    cellular protein complex assembly GO:0043623 ISS
    negative regulation of neuron death GO:1901215 TAS
    regulation of immunoglobulin secretion GO:0051023 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
    protein autoubiquitination GO:0051865 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    protein catabolic process GO:0030163 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IMP
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    death-inducing signaling complex assembly GO:0071550 TAS
    regulation of apoptotic process GO:0042981 IDA
    positive regulation of T cell activation GO:0050870 IC
    necroptotic process GO:0070266 TAS
    apoptotic process GO:0006915 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    protein complex assembly GO:0006461 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    protein homotrimerization GO:0070207 IPI
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    positive regulation of protein homodimerization activity GO:0090073 IMP
    positive regulation of JUN kinase activity GO:0043507 IDA
    response to endoplasmic reticulum stress GO:0034976 NAS
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    programmed necrotic cell death GO:0097300 TAS
    programmed cell death GO:0012501 TAS
    protein K63-linked ubiquitination GO:0070534 IDA
    regulation of necrotic cell death GO:0010939 TAS
    cellular response to nitric oxide GO:0071732 IEA
Subcellular Localization
    cell cortex GO:0005938 IEA
    cytosol GO:0005829 TAS
    ubiquitin ligase complex GO:0000151 IPI
    cytoplasmic side of plasma membrane GO:0009898 ISS
    IRE1-TRAF2-ASK1 complex GO:1990604 IDA
    vesicle membrane GO:0012506 IEA
    CD40 receptor complex GO:0035631 ISS
    membrane raft GO:0045121 IEA
    cytoplasm GO:0005737 IDA
    TRAF2-GSTP1 complex GO:0097057 IDA
 Experiment description of studies that identified TRAF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TRAF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNFAIP3 7128
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 BAHD1  
Two-hybrid Homo sapiens
3 AFF4 27125
Two-hybrid Homo sapiens
4 ERN1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 QARS 5859
Two-hybrid Homo sapiens
6 IRF4  
Two-hybrid Homo sapiens
7 SIAH2 6478
Reconstituted Complex Homo sapiens
8 NDP52  
Two-hybrid Homo sapiens
9 MAP4K2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 TNFRSF7  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 TNFRSF8 943
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 TAB1 10454
Affinity Capture-MS Homo sapiens
13 TU3A  
Two-hybrid Homo sapiens
14 RIPK1 8737
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 SOX9  
Invitro Homo sapiens
16 TNFSF4  
Two-hybrid Homo sapiens
17 DLG7  
Two-hybrid Homo sapiens
18 RIPK3 11035
Invivo Homo sapiens
Two-hybrid Homo sapiens
19 MAP3K5 4217
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 C16orf48  
Two-hybrid Homo sapiens
21 KIAA1267  
Two-hybrid Homo sapiens
22 TAOK3 51347
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
23 ABLIM1 3983
Two-hybrid Homo sapiens
24 TNIK 23043
Affinity Capture-Western Homo sapiens
25 FBXO28  
Two-hybrid Homo sapiens
26 MAP4K5 11183
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
27 TNFRSF19  
Invivo Homo sapiens
28 CHMP2B 25978
Two-hybrid Homo sapiens
29 CCDC33 80125
Two-hybrid Homo sapiens
30 TNFRSF12A 51330
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
31 Tnfrsf14  
Two-hybrid Mus musculus
32 TCEB3B  
Two-hybrid Homo sapiens
33 TNFSF9  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 BIRC3 330
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 SETD5 55209
Two-hybrid Homo sapiens
36 AASDHPPT 60496
Two-hybrid Homo sapiens
37 LOC116349  
Two-hybrid Homo sapiens
38 USP2  
Two-hybrid Homo sapiens
39 USP7 7874
Invitro Homo sapiens
40 TNFRSF1B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 USP53 54532
Two-hybrid Homo sapiens
43 NIF3L1 60491
Two-hybrid Homo sapiens
44 RAD23A 5886
Two-hybrid Homo sapiens
45 TNFRSF4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
46 BANP  
Two-hybrid Homo sapiens
47 SPG21 51324
Two-hybrid Homo sapiens
48 IPO11 51194
Two-hybrid Homo sapiens
49 TBK1 29110
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 CRY1  
Two-hybrid Homo sapiens
51 MGC12538  
Two-hybrid Homo sapiens
52 RB1CC1 9821
Affinity Capture-Western Homo sapiens
53 ZBTB16  
Two-hybrid Homo sapiens
54 TNFRSF14  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 TAB3 257397
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
56 TRIM31  
Two-hybrid Homo sapiens
57 TCEA2  
Two-hybrid Homo sapiens
58 TNFRSF17  
Invivo Homo sapiens
59 NECAP2 55707
Two-hybrid Homo sapiens
60 MIZF  
Two-hybrid Homo sapiens
61 RFX3 5991
Two-hybrid Homo sapiens
62 NGFR 4804
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 DTNB 1838
Two-hybrid Homo sapiens
64 HIVEP3 59269
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
65 GORASP2 26003
Two-hybrid Homo sapiens
66 SPATA2  
Two-hybrid Homo sapiens
67 SOX30  
Two-hybrid Homo sapiens
68 RGS14  
Two-hybrid Homo sapiens
69 ZBTB43  
Two-hybrid Homo sapiens
70 TNFRSF13B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
71 MGC10471  
Two-hybrid Homo sapiens
72 TNF  
Affinity Capture-Western Homo sapiens
73 BIRC2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
74 CD40 958
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
75 IL15RA 3601
Affinity Capture-Western Homo sapiens
76 SOC  
Two-hybrid Homo sapiens
77 SPHK1 8877
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
78 TRADD 8717
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 CDCA3 83461
Two-hybrid Homo sapiens
80 FLJ22494  
Two-hybrid Homo sapiens
81 NGFRAP1  
Affinity Capture-MS Homo sapiens
82 TDP2 51567
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
83 TNFRSF1A 7132
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
84 TRAF6 7189
Two-hybrid Homo sapiens
85 TNFRSF9  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
86 MAP3K1 4214
Invivo Homo sapiens
87 MAP3K8  
Affinity Capture-RNA Homo sapiens
88 MAP3K7  
Affinity Capture-Western Homo sapiens
89 TNFRSF18  
Affinity Capture-Western Homo sapiens
90 LZTS2 84445
Two-hybrid Homo sapiens
91 FAM120B 84498
Two-hybrid Homo sapiens
92 FXR2 9513
Two-hybrid Homo sapiens
93 LNX  
Two-hybrid Homo sapiens
94 RIPK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
95 ZBTB25  
Two-hybrid Homo sapiens
96 TRAF2 7186
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
97 RIBC2  
Two-hybrid Homo sapiens
98 ITPK1 3705
Two-hybrid Homo sapiens
99 APEX2  
Two-hybrid Homo sapiens
100 FBF1  
Two-hybrid Homo sapiens
101 CDKN1B 1027
Two-hybrid Homo sapiens
102 FATE1  
Two-hybrid Homo sapiens
103 CHUK 1147
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
104 ZA20D3  
Two-hybrid Homo sapiens
105 DVL2 1856
Two-hybrid Homo sapiens
106 MAP3K14  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
107 TANK 10010
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
108 TRAF3IP2  
Two-hybrid Homo sapiens
109 TIFA  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
110 APPL1 26060
Two-hybrid Homo sapiens
111 SPRY2 10253
Two-hybrid Homo sapiens
112 FLNA  
Affinity Capture-Western Homo sapiens
113 PIN1 5300
Two-hybrid Homo sapiens
114 TNFSF14  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
115 CASP8 841
Affinity Capture-Western Homo sapiens
116 TRIP  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
117 BCL10  
Invivo Homo sapiens
Two-hybrid Homo sapiens
118 THAP7  
Two-hybrid Homo sapiens
119 MAST2  
Affinity Capture-Western Homo sapiens
120 TNFRSF11A  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
121 ZNF646  
Two-hybrid Homo sapiens
122 TRIM37  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
123 ZNF205 7755
Two-hybrid Homo sapiens
124 MGC17403  
Two-hybrid Homo sapiens
125 MAPK9 5601
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
126 CDK9 1025
Invivo Homo sapiens
Two-hybrid Homo sapiens
127 PSMF1 9491
Two-hybrid Homo sapiens
128 TRPT1 83707
Two-hybrid Homo sapiens
129 NRIP1 8204
Two-hybrid Homo sapiens
130 CASP8AP2  
Invivo Homo sapiens
Invitro Homo sapiens
131 IKBKB 3551
Reconstituted Complex Homo sapiens
132 CAV1 857
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
133 KIAA0408  
Two-hybrid Homo sapiens
134 TRPC4AP  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
135 TRAF1 7185
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
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