Gene description for PTPN2
Gene name protein tyrosine phosphatase, non-receptor type 2
Gene symbol PTPN2
Other names/aliases PTN2
PTPT
TC-PTP
TCELLPTP
TCPTP
Species Homo sapiens
 Database cross references - PTPN2
ExoCarta ExoCarta_5771
Vesiclepedia VP_5771
Entrez Gene 5771
HGNC 9650
MIM 176887
UniProt P17706  
 PTPN2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PTPN2
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 EXP
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 IMP
    protein tyrosine phosphatase activity GO:0004725 TAS
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IBA
    integrin binding GO:0005178 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    syntaxin binding GO:0019905 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    receptor tyrosine kinase binding GO:0030971 IPI
    STAT family protein binding GO:0097677 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    chromatin remodeling GO:0006338 IEA
    negative regulation of cell population proliferation GO:0008285 IMP
    insulin receptor signaling pathway GO:0008286 ISS
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 ISS
    negative regulation of lipid storage GO:0010888 ISS
    B cell differentiation GO:0030183 ISS
    T cell differentiation GO:0030217 ISS
    erythrocyte differentiation GO:0030218 ISS
    peptidyl-tyrosine dephosphorylation GO:0035335 IMP
    insulin receptor recycling GO:0038020 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IMP
    negative regulation of tyrosine phosphorylation of STAT protein GO:0042532 IDA
    glucose homeostasis GO:0042593 ISS
    negative regulation of macrophage differentiation GO:0045650 ISS
    positive regulation of gluconeogenesis GO:0045722 ISS
    negative regulation of receptor signaling pathway via JAK-STAT GO:0046426 IBA
    negative regulation of insulin receptor signaling pathway GO:0046627 ISS
    negative regulation of inflammatory response GO:0050728 ISS
    negative regulation of T cell receptor signaling pathway GO:0050860 ISS
    negative regulation of chemotaxis GO:0050922 ISS
    regulation of type II interferon-mediated signaling pathway GO:0060334 TAS
    negative regulation of type II interferon-mediated signaling pathway GO:0060336 IDA
    negative regulation of type II interferon-mediated signaling pathway GO:0060336 IMP
    negative regulation of type II interferon-mediated signaling pathway GO:0060336 ISS
    negative regulation of type I interferon-mediated signaling pathway GO:0060339 IMP
    negative regulation of interleukin-6-mediated signaling pathway GO:0070104 IEA
    negative regulation of interleukin-6-mediated signaling pathway GO:0070104 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IBA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    regulation of hepatocyte growth factor receptor signaling pathway GO:1902202 IMP
    negative regulation of interleukin-2-mediated signaling pathway GO:1902206 IMP
    negative regulation of interleukin-4-mediated signaling pathway GO:1902215 IEA
    negative regulation of interleukin-4-mediated signaling pathway GO:1902215 IMP
    negative regulation of macrophage colony-stimulating factor signaling pathway GO:1902227 ISS
    negative regulation of positive thymic T cell selection GO:1902233 ISS
    positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902237 IEA
    positive regulation of PERK-mediated unfolded protein response GO:1903899 IEA
    negative regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000587 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    endosome lumen GO:0031904 IEA
 Experiment description of studies that identified PTPN2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTPN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STRA6 64220
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TFB1M  
Affinity Capture-MS Homo sapiens
5 OCLN 100506658
Biochemical Activity Homo sapiens
6 PSMD6 9861
Affinity Capture-MS Homo sapiens
7 KPNA1 3836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 METTL7A 25840
Proximity Label-MS Homo sapiens
9 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SPRYD4 283377
Affinity Capture-MS Homo sapiens
11 RCL1 10171
Affinity Capture-MS Homo sapiens
12 ACKR2  
Affinity Capture-MS Homo sapiens
13 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
14 DBN1 1627
Affinity Capture-MS Homo sapiens
15 NMNAT1  
Affinity Capture-MS Homo sapiens
16 UBE3A 7337
Affinity Capture-MS Homo sapiens
17 GJD3  
Proximity Label-MS Homo sapiens
18 UBC 7316
Reconstituted Complex Homo sapiens
19 DNAJC25 548645
Proximity Label-MS Homo sapiens
20 PSMD10 5716
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PTPN1 5770
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
22 TNFSF18 8995
Affinity Capture-MS Homo sapiens
23 APP 351
Affinity Capture-MS Homo sapiens
24 PTPN20B  
Affinity Capture-MS Homo sapiens
25 DIMT1 27292
Affinity Capture-MS Homo sapiens
26 TMOD3 29766
Affinity Capture-MS Homo sapiens
27 DNAJC10 54431
Affinity Capture-MS Homo sapiens
28 COIL  
Affinity Capture-MS Homo sapiens
29 CTLA4  
Affinity Capture-MS Homo sapiens
30 COL5A1 1289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 VARS 7407
Co-fractionation Homo sapiens
32 ANP32B 10541
Affinity Capture-MS Homo sapiens
33 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
34 STAT3 6774
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 PSMC6 5706
Affinity Capture-MS Homo sapiens
36 SSR1 6745
Proximity Label-MS Homo sapiens
37 PSMC2 5701
Affinity Capture-MS Homo sapiens
38 ANP32C 23520
Affinity Capture-MS Homo sapiens
39 EXOSC4 54512
Affinity Capture-MS Homo sapiens
40 DNAJC16  
Proximity Label-MS Homo sapiens
41 KATNB1  
Co-fractionation Homo sapiens
42 UCHL5 51377
Affinity Capture-MS Homo sapiens
43 SFN 2810
Affinity Capture-MS Homo sapiens
44 TMEM132D  
Two-hybrid Homo sapiens
45 STAT6 6778
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
46 FAM20C 56975
Affinity Capture-MS Homo sapiens
47 GAR1 54433
Affinity Capture-MS Homo sapiens
48 ADARB2-AS1  
Affinity Capture-MS Homo sapiens
49 METTL13 51603
Co-fractionation Homo sapiens
50 KIAA1715 80856
Proximity Label-MS Homo sapiens
51 PXMP2  
Proximity Label-MS Homo sapiens
52 LRRC59 55379
Proximity Label-MS Homo sapiens
53 EMD 2010
Proximity Label-MS Homo sapiens
54 PSMD4 5710
Affinity Capture-MS Homo sapiens
55 GTPBP10  
Affinity Capture-MS Homo sapiens
56 ERGIC2 51290
Proximity Label-MS Homo sapiens
57 PSMC1 5700
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PSMD14 10213
Affinity Capture-MS Homo sapiens
59 PNO1 56902
Affinity Capture-MS Homo sapiens
60 APLP2 334
Affinity Capture-MS Homo sapiens
61 PSMD2 5708
Affinity Capture-MS Homo sapiens
62 PCYT2 5833
Co-fractionation Homo sapiens
63 ZC3HAV1L 92092
Affinity Capture-MS Homo sapiens
64 RPN1 6184
Proximity Label-MS Homo sapiens
65 TSPAN31 6302
Affinity Capture-MS Homo sapiens
66 KPNA5 3841
Affinity Capture-MS Homo sapiens
67 CSPG5 10675
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SEC61B 10952
Proximity Label-MS Homo sapiens
69 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
70 SEC63 11231
Proximity Label-MS Homo sapiens
71 CASC4 113201
Affinity Capture-MS Homo sapiens
72 HADHA 3030
Affinity Capture-MS Homo sapiens
73 RPN2 6185
Proximity Label-MS Homo sapiens
74 PTPN6 5777
Co-fractionation Homo sapiens
75 PANX1 24145
Proximity Label-MS Homo sapiens
76 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PSMD8 5714
Affinity Capture-MS Homo sapiens
78 BCAP31 10134
Proximity Label-MS Homo sapiens
79 HSD3B7 80270
Proximity Label-MS Homo sapiens
80 RPP30 10556
Affinity Capture-MS Homo sapiens
81 GADD45GIP1  
Affinity Capture-MS Homo sapiens
82 ANP32E 81611
Affinity Capture-MS Homo sapiens
83 CORO1C 23603
Affinity Capture-MS Homo sapiens
84 MYO1C 4641
Affinity Capture-MS Homo sapiens
85 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
86 ATP2A1 487
Proximity Label-MS Homo sapiens
87 RAB9A 9367
Proximity Label-MS Homo sapiens
88 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 GHR  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
90 TXNDC5 81567
Affinity Capture-MS Homo sapiens
91 HSPD1 3329
Co-fractionation Homo sapiens
92 LMNA 4000
Proximity Label-MS Homo sapiens
93 FTSJ3 117246
Affinity Capture-MS Homo sapiens
94 TPM4 7171
Affinity Capture-MS Homo sapiens
95 EIF2S1 1965
Affinity Capture-MS Homo sapiens
96 PSMC3IP  
Affinity Capture-MS Homo sapiens
97 C8orf33 65265
Affinity Capture-MS Homo sapiens
98 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
99 RPL3L 6123
Affinity Capture-MS Homo sapiens
100 RBM34  
Affinity Capture-MS Homo sapiens
101 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 PARP1 142
Proximity Label-MS Homo sapiens
103 DUSP10 11221
Affinity Capture-MS Homo sapiens
104 ASGR1 432
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CALU 813
Affinity Capture-MS Homo sapiens
107 BYSL 705
Affinity Capture-MS Homo sapiens
108 KPNA6 23633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 HMGN5 79366
Affinity Capture-MS Homo sapiens
110 TRMT61B  
Affinity Capture-MS Homo sapiens
111 G3BP1 10146
Affinity Capture-MS Homo sapiens
112 B4GAT1 11041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NUP155 9631
Proximity Label-MS Homo sapiens
114 ERGIC1 57222
Proximity Label-MS Homo sapiens
115 ELOVL5 60481
Proximity Label-MS Homo sapiens
116 BRIX1 55299
Affinity Capture-MS Homo sapiens
117 GOLIM4 27333
Affinity Capture-MS Homo sapiens
118 NUP50 10762
Affinity Capture-MS Homo sapiens
119 SETD7 80854
Affinity Capture-MS Homo sapiens
120 RNMTL1  
Affinity Capture-MS Homo sapiens
121 SEC62 7095
Proximity Label-MS Homo sapiens
122 HARS 3035
Affinity Capture-MS Homo sapiens
123 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 CASKIN1  
Affinity Capture-MS Homo sapiens
125 GRSF1 2926
Affinity Capture-MS Homo sapiens
126 EIF5A 1984
Affinity Capture-MS Homo sapiens
127 DNAJC1 64215
Proximity Label-MS Homo sapiens
128 PSMD1 5707
Affinity Capture-MS Homo sapiens
129 CKAP4 10970
Proximity Label-MS Homo sapiens
130 PSMC4 5704
Affinity Capture-MS Homo sapiens
131 NSUN5  
Affinity Capture-MS Homo sapiens
132 DERL1 79139
Proximity Label-MS Homo sapiens
133 PSMD13 5719
Affinity Capture-MS Homo sapiens
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