Gene description for RPL3L
Gene name ribosomal protein L3-like
Gene symbol RPL3L
Other names/aliases -
Species Homo sapiens
 Database cross references - RPL3L
ExoCarta ExoCarta_6123
Vesiclepedia VP_6123
Entrez Gene 6123
HGNC 10351
UniProt Q92901  
 RPL3L identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RPL3L
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 NAS
Biological Process
    translation GO:0006412 IBA
    translation GO:0006412 NAS
    negative regulation of myotube differentiation GO:0010832 IEA
    regulation of striated muscle tissue development GO:0016202 IEA
Subcellular Localization
    ribosome GO:0005840 TAS
    membrane GO:0016020 HDA
    cytosolic large ribosomal subunit GO:0022625 HDA
    cytosolic large ribosomal subunit GO:0022625 IBA
 Experiment description of studies that identified RPL3L in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RPL3L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Co-fractionation Homo sapiens
2 TERT  
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 RPL9 6133
Co-fractionation Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 RPS25 6230
Co-fractionation Homo sapiens
7 PABPC4 8761
Co-fractionation Homo sapiens
8 RPL19 6143
Co-fractionation Homo sapiens
9 RPL23 9349
Co-fractionation Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 RPS2 6187
Co-fractionation Homo sapiens
12 RPL13 6137
Co-fractionation Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 RPL30 6156
Co-fractionation Homo sapiens
15 ECT2 1894
Affinity Capture-MS Homo sapiens
16 RPL6 6128
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 RPS26 6231
Co-fractionation Homo sapiens
18 CHMP4C 92421
Affinity Capture-MS Homo sapiens
19 RPS23 6228
Co-fractionation Homo sapiens
20 RPL27 6155
Co-fractionation Homo sapiens
21 RPL37A 6168
Co-fractionation Homo sapiens
22 RPL3 6122
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
23 CIT 11113
Affinity Capture-MS Homo sapiens
24 RPL21 6144
Co-fractionation Homo sapiens
25 PTP4A1 7803
Affinity Capture-MS Homo sapiens
26 RPL14 9045
Co-fractionation Homo sapiens
27 PTPN2 5771
Affinity Capture-MS Homo sapiens
28 RPL4 6124
Co-fractionation Homo sapiens
29 G3BP2 9908
Affinity Capture-MS Homo sapiens
30 ZBTB47  
Affinity Capture-MS Homo sapiens
31 RPL7 6129
Co-fractionation Homo sapiens
32 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
33 ILF2 3608
Affinity Capture-MS Homo sapiens
34 AURKB 9212
Affinity Capture-MS Homo sapiens
35 RPS18 6222
Co-fractionation Homo sapiens
36 RPL39 6170
Co-fractionation Homo sapiens
37 RPL5 6125
Co-fractionation Homo sapiens
38 RPS20 6224
Co-fractionation Homo sapiens
39 RPL18A 6142
Co-fractionation Homo sapiens
40 PRC1 9055
Affinity Capture-MS Homo sapiens
41 RPL7A 6130
Co-fractionation Homo sapiens
42 RPS6 6194
Co-fractionation Homo sapiens
43 RPL8 6132
Co-fractionation Homo sapiens
44 RPL27A 6157
Co-fractionation Homo sapiens
45 RPL36 25873
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
46 RPL24 6152
Co-fractionation Homo sapiens
47 RPL23A 6147
Co-fractionation Homo sapiens
48 RPL18 6141
Co-fractionation Homo sapiens
49 RPS8 6202
Co-fractionation Homo sapiens
50 MTMR2 8898
Affinity Capture-MS Homo sapiens
51 RPLP0 6175
Cross-Linking-MS (XL-MS) Homo sapiens
52 RPS9 6203
Co-fractionation Homo sapiens
53 ZXDB  
Affinity Capture-MS Homo sapiens
54 RPL15 6138
Co-fractionation Homo sapiens
55 RPS16 6217
Co-fractionation Homo sapiens
56 LOC101929876 101929876
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which RPL3L is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Eukaryotic Translation Elongation IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
Eukaryotic Translation Termination IEA Reactome
Formation of a pool of free 40S subunits TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Major pathway of rRNA processing in the nucleolus and cytosol TAS Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Peptide chain elongation IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
rRNA processing TAS Reactome
rRNA processing in the nucleus and cytosol TAS Reactome
Selenoamino acid metabolism IEA Reactome
Selenocysteine synthesis IEA Reactome
Signaling by ROBO receptors IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
Translation TAS Reactome
Translation IEA Reactome
Viral Infection Pathways TAS Reactome
Viral mRNA Translation TAS Reactome





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