Gene description for RPL39
Gene name ribosomal protein L39
Gene symbol RPL39
Other names/aliases L39
RPL39P42
RPL39_23_1806
Species Homo sapiens
 Database cross references - RPL39
ExoCarta ExoCarta_6170
Vesiclepedia VP_6170
Entrez Gene 6170
HGNC 10350
MIM 300899
UniProt P62891  
 RPL39 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RPL39
Molecular Function
    RNA binding GO:0003723 TAS
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 NAS
    structural constituent of ribosome GO:0003735 TAS
Biological Process
    cytoplasmic translation GO:0002181 IC
    cytoplasmic translation GO:0002181 IDA
    innate immune response in mucosa GO:0002227 IDA
    translation GO:0006412 NAS
    translation GO:0006412 TAS
    antibacterial humoral response GO:0019731 IDA
    defense response to Gram-positive bacterium GO:0050830 IDA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 TAS
    cytosolic large ribosomal subunit GO:0022625 HDA
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 TAS
    cytosolic ribosome GO:0022626 IDA
 Experiment description of studies that identified RPL39 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RPL39
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 DDX6 1656
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 TLX2  
Affinity Capture-MS Homo sapiens
5 PDZD8 118987
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 PER2  
Proximity Label-MS Homo sapiens
8 ZBTB2 57621
Affinity Capture-MS Homo sapiens
9 SOX5 6660
Affinity Capture-MS Homo sapiens
10 PABPC4 8761
Co-fractionation Homo sapiens
11 NKX2-5  
Affinity Capture-MS Homo sapiens
12 RPS3A 6189
Co-fractionation Homo sapiens
13 CTCF  
Affinity Capture-MS Homo sapiens
14 EIF2S3 1968
Affinity Capture-MS Homo sapiens
15 SMURF1 57154
Affinity Capture-MS Homo sapiens
16 EMC9  
Affinity Capture-MS Homo sapiens
17 CPEB1  
Affinity Capture-MS Homo sapiens
18 KLF16  
Affinity Capture-MS Homo sapiens
19 SSB 6741
Affinity Capture-MS Homo sapiens
20 KLF5  
Affinity Capture-MS Homo sapiens
21 RPL37A 6168
Co-fractionation Homo sapiens
22 SRP9 6726
Affinity Capture-MS Homo sapiens
23 HNRNPA2B1 3181
Co-fractionation Homo sapiens
24 USP36  
Affinity Capture-MS Homo sapiens
25 BUB1B  
Affinity Capture-MS Homo sapiens
26 DDX21 9188
Affinity Capture-MS Homo sapiens
27 RPS15 6209
Co-fractionation Homo sapiens
28 RPL9 6133
Co-fractionation Homo sapiens
29 EGFR 1956
Negative Genetic Homo sapiens
30 CAPZB 832
Affinity Capture-MS Homo sapiens
31 DRG1 4733
Affinity Capture-MS Homo sapiens
32 MYC  
Affinity Capture-MS Homo sapiens
33 SOX2  
Affinity Capture-MS Homo sapiens
34 RPS4X 6191
Co-fractionation Homo sapiens
35 GSPT1 2935
Affinity Capture-MS Homo sapiens
36 FTSJ3 117246
Co-fractionation Homo sapiens
37 RPL32 6161
Co-fractionation Homo sapiens
38 RPS18 6222
Co-fractionation Homo sapiens
39 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
40 RPL3L 6123
Co-fractionation Homo sapiens
41 RPL5 6125
Affinity Capture-MS Homo sapiens
42 NCAPH 23397
Affinity Capture-MS Homo sapiens
43 PABPC1 26986
Co-fractionation Homo sapiens
44 PSPC1 55269
Affinity Capture-MS Homo sapiens
45 RBM3 5935
Affinity Capture-MS Homo sapiens
46 KLF8  
Affinity Capture-MS Homo sapiens
47 RPL23 9349
Co-fractionation Homo sapiens
48 ATG13 9776
Affinity Capture-MS Homo sapiens
49 RPL35A 6165
Co-fractionation Homo sapiens
50 EIF3B 8662
Affinity Capture-MS Homo sapiens
51 METAP2 10988
Affinity Capture-MS Homo sapiens
52 KRAS 3845
Negative Genetic Homo sapiens
53 EIF5 1983
Affinity Capture-MS Homo sapiens
54 ENY2 56943
Affinity Capture-MS Homo sapiens
55 KLF15  
Affinity Capture-MS Homo sapiens
56 FOLR1 2348
Affinity Capture-MS Homo sapiens
57 RPS16 6217
Affinity Capture-MS Homo sapiens
58 RPS9 6203
Co-fractionation Homo sapiens
59 MYCN  
Affinity Capture-MS Homo sapiens
60 ARAF 369
Affinity Capture-MS Homo sapiens
61 KLF12  
Proximity Label-MS Homo sapiens
62 SEC61B 10952
Affinity Capture-MS Homo sapiens
63 ILF3 3609
Affinity Capture-MS Homo sapiens
64 CDC5L 988
Co-fractionation Homo sapiens
65 HMGB2 3148
Affinity Capture-MS Homo sapiens
66 SRP68 6730
Affinity Capture-MS Homo sapiens
67 RPL19 6143
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RPL39 is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Eukaryotic Translation Elongation IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
Eukaryotic Translation Termination IEA Reactome
Formation of a pool of free 40S subunits TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Major pathway of rRNA processing in the nucleolus and cytosol TAS Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Peptide chain elongation IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
rRNA processing TAS Reactome
rRNA processing in the nucleus and cytosol TAS Reactome
Selenoamino acid metabolism IEA Reactome
Selenocysteine synthesis IEA Reactome
Signaling by ROBO receptors IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
Translation TAS Reactome
Translation IEA Reactome
Viral Infection Pathways TAS Reactome
Viral mRNA Translation TAS Reactome





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