Gene description for RPS23
Gene name ribosomal protein S23
Gene symbol RPS23
Other names/aliases S23
Species Homo sapiens
 Database cross references - RPS23
ExoCarta ExoCarta_6228
Entrez Gene 6228
HGNC 10410
MIM 603683
UniProt P62266  
 RPS23 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RPS23
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    structural constituent of ribosome GO:0003735 IEA
    protein binding GO:0005515 IPI
Biological Process
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    translation GO:0006412 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    viral process GO:0016032 TAS
    viral life cycle GO:0019058 TAS
    translational termination GO:0006415 TAS
    translational initiation GO:0006413 TAS
    viral transcription GO:0019083 TAS
    gene expression GO:0010467 TAS
    translational elongation GO:0006414 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified RPS23 in exosomes
1
Experiment ID 282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS23
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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