Experiment description of studies that identified EIF3CL in exosomes

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 219 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name C666-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 220 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name NP69 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 221 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name NP460 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for EIF3CL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 EIF3A 8661 Co-fractionation Homo sapiens Co-fractionation Homo sapiens 2 ANLN 54443 Affinity Capture-MS Homo sapiens 3 RIT1 6016 Negative Genetic Homo sapiens 4 NUDT21 11051 Co-fractionation Homo sapiens 5 EIF3F 8665 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 DDX6 1656 Affinity Capture-MS Homo sapiens 7 RPS11 6205 Co-fractionation Homo sapiens 8 EIF3C 8663 Affinity Capture-MS Homo sapiens 9 RPS3A 6189 Co-fractionation Homo sapiens 10 CTCF   Affinity Capture-MS Homo sapiens 11 KIF14 9928 Affinity Capture-MS Homo sapiens 12 RPS2 6187 Co-fractionation Homo sapiens 13 CIT 11113 Affinity Capture-MS Homo sapiens 14 EIF3K 27335 Co-fractionation Homo sapiens 15 EIF3I 8668 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 ECT2 1894 Affinity Capture-MS Homo sapiens 17 DYNC1I2 1781 Co-fractionation Homo sapiens 18 RPL35 11224 Co-fractionation Homo sapiens 19 RPS23 6228 Co-fractionation Homo sapiens 20 Itsn2   Affinity Capture-MS Mus musculus 21 ATG13 9776 Affinity Capture-MS Homo sapiens 22 RPS12 6206 Co-fractionation Homo sapiens 23 MIB1 57534 Proximity Label-MS Homo sapiens 24 CUL3 8452 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 RPS4X 6191 Co-fractionation Homo sapiens 26 CAPRIN1 4076 Affinity Capture-MS Homo sapiens 27 EIF3G 8666 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 28 CLTB 1212 Affinity Capture-MS Homo sapiens 29 RPS5 6193 Co-fractionation Homo sapiens 30 OCRL 4952 Affinity Capture-MS Homo sapiens 31 RPS3 6188 Co-fractionation Homo sapiens 32 RPS26 6231 Co-fractionation Homo sapiens 33 PDZD8 118987 Affinity Capture-MS Homo sapiens 34 UFL1 23376 Affinity Capture-MS Homo sapiens 35 RPS18 6222 Co-fractionation Homo sapiens 36 RPS27 6232 Co-fractionation Homo sapiens 37 NCAPH 23397 Affinity Capture-MS Homo sapiens 38 EIF5 1983 Affinity Capture-MS Homo sapiens 39 RPL4 6124 Affinity Capture-MS Homo sapiens 40 RPS20 6224 Co-fractionation Homo sapiens 41 METAP2 10988 Affinity Capture-MS Homo sapiens 42 RPS24 6229 Co-fractionation Homo sapiens 43 VCP 7415 Affinity Capture-MS Homo sapiens 44 MCM2 4171 Affinity Capture-MS Homo sapiens 45 CLTA 1211 Affinity Capture-MS Homo sapiens 46 RPS6 6194 Co-fractionation Homo sapiens 47 EIF3B 8662 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 48 EIF2S3 1968 Affinity Capture-MS Homo sapiens 49 IMPDH2 3615 Proximity Label-MS Homo sapiens 50 DHFRL1   Proximity Label-MS Homo sapiens 51 RPL23A 6147 Co-fractionation Homo sapiens 52 DDRGK1 65992 Affinity Capture-MS Homo sapiens 53 EIF3M 10480 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 54 KRAS 3845 Negative Genetic Homo sapiens 55 ENY2 56943 Affinity Capture-MS Homo sapiens 56 CKAP4 10970 Proximity Label-MS Homo sapiens 57 HMGB2 3148 Affinity Capture-MS Homo sapiens 58 EMC9   Affinity Capture-MS Homo sapiens 59 GSPT1 2935 Affinity Capture-MS Homo sapiens 60 SND1 27044 Co-fractionation Homo sapiens 61 BRD3 8019 Affinity Capture-MS Homo sapiens 62 EIF3E 3646 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 63 C9orf72   Affinity Capture-MS Homo sapiens 64 APPL1 26060 Affinity Capture-MS Homo sapiens 65 EIF3D 8664 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 66 EIF3H 8667 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 EIF3L 51386 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which EIF3CL is involved

No pathways found