Gene description for MIB1
Gene name mindbomb E3 ubiquitin protein ligase 1
Gene symbol MIB1
Other names/aliases DIP-1
DIP1
LVNC7
MIB
ZZANK2
ZZZ6
Species Homo sapiens
 Database cross references - MIB1
ExoCarta ExoCarta_57534
Entrez Gene 57534
HGNC 21086
MIM 608677
UniProt Q86YT6  
 MIB1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for MIB1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    positive regulation of endocytosis GO:0045807 IEA
    somitogenesis GO:0001756 IEA
    heart looping GO:0001947 IEA
    negative regulation of neuron differentiation GO:0045665 IEA
    Notch signaling pathway GO:0007219 TAS
    blood vessel development GO:0001568 IEA
    protein ubiquitination GO:0016567 IEA
    endocytosis GO:0006897 IBA
    in utero embryonic development GO:0001701 IEA
    neural tube formation GO:0001841 IEA
Subcellular Localization
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 IBA
    postsynaptic density GO:0014069 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified MIB1 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for MIB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DAPK1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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