Gene description for ASCC3
Gene name activating signal cointegrator 1 complex subunit 3
Gene symbol ASCC3
Other names/aliases ASC1p200
HELIC1
RNAH
Species Homo sapiens
 Database cross references - ASCC3
ExoCarta ExoCarta_10973
Entrez Gene 10973
HGNC 18697
MIM 614217
UniProt Q8N3C0  
 ASCC3 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for ASCC3
Molecular Function
    ATP-dependent 3'-5' DNA helicase activity GO:0043140 IDA
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    DNA repair GO:0006281 TAS
    DNA duplex unwinding GO:0032508 IDA
    cell proliferation GO:0008283 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    DNA dealkylation involved in DNA repair GO:0006307 TAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IDA
    intracellular GO:0005622 IDA
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ASCC3 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASCC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP4  
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 ASCC2  
Invivo Homo sapiens
3 ASCC1 51008
Invivo Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ASCC3 is involved
PathwayEvidenceSource
ALKBH3 mediated reversal of alkylation damage TAS Reactome





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