Gene description for RIOK3
Gene name RIO kinase 3
Gene symbol RIOK3
Other names/aliases SUDD
Species Homo sapiens
 Database cross references - RIOK3
ExoCarta ExoCarta_8780
Vesiclepedia VP_8780
Entrez Gene 8780
HGNC 11451
MIM 603579
UniProt O14730  
 RIOK3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for RIOK3
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    caspase binding GO:0089720 IDA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    chromosome segregation GO:0007059 TAS
    maturation of SSU-rRNA GO:0030490 IBA
    maturation of SSU-rRNA GO:0030490 IMP
    negative regulation of protein-containing complex assembly GO:0031333 IDA
    positive regulation of interferon-beta production GO:0032728 IMP
    negative regulation of MDA-5 signaling pathway GO:0039534 IMP
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IMP
    defense response to virus GO:0051607 IEA
    cellular response to dsRNA GO:0071359 IMP
    cellular response to virus GO:0098586 IMP
    cellular response to dsDNA GO:1990786 IMP
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    preribosome, small subunit precursor GO:0030688 IBA
    preribosome, small subunit precursor GO:0030688 IDA
 Experiment description of studies that identified RIOK3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RIOK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSR4 6748
Affinity Capture-MS Homo sapiens
2 FBL 2091
Affinity Capture-MS Homo sapiens
3 RPSA 3921
Affinity Capture-MS Homo sapiens
4 RPS27A 6233
Affinity Capture-MS Homo sapiens
5 HUWE1 10075
Affinity Capture-MS Homo sapiens
6 ASCC3 10973
Affinity Capture-MS Homo sapiens
7 RPS19 6223
Affinity Capture-MS Homo sapiens
8 RPS25 6230
Affinity Capture-MS Homo sapiens
9 RPS11 6205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RPS4X 6191
Affinity Capture-MS Homo sapiens
11 RPS3A 6189
Affinity Capture-MS Homo sapiens
12 RPS2 6187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 RPS26 6231
Affinity Capture-MS Homo sapiens
15 RPS17 6218
Affinity Capture-MS Homo sapiens
16 RUVBL2 10856
Affinity Capture-MS Homo sapiens
17 ZMAT3  
Affinity Capture-MS Homo sapiens
18 FBXO25  
Biochemical Activity Homo sapiens
19 PAK2 5062
Two-hybrid Homo sapiens
20 PNO1 56902
Affinity Capture-MS Homo sapiens
21 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 SMURF1 57154
Biochemical Activity Homo sapiens
23 SQSTM1 8878
Proximity Label-MS Homo sapiens
24 RPS15 6209
Affinity Capture-MS Homo sapiens
25 BCL2L11  
Two-hybrid Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 RPS28 6234
Affinity Capture-MS Homo sapiens
28 RPS10 6204
Affinity Capture-MS Homo sapiens
29 MYO1C 4641
Affinity Capture-MS Homo sapiens
30 ADAM7  
Affinity Capture-MS Homo sapiens
31 MMGT1 93380
Affinity Capture-MS Homo sapiens
32 CDC42 998
Two-hybrid Homo sapiens
33 UBIAD1 29914
Affinity Capture-MS Homo sapiens
34 RPS18 6222
Affinity Capture-MS Homo sapiens
35 KRR1 11103
Affinity Capture-MS Homo sapiens
36 KCNE3  
Affinity Capture-MS Homo sapiens
37 RPS20 6224
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RPS24 6229
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MOV10 4343
Affinity Capture-RNA Homo sapiens
40 SUPT6H 6830
Affinity Capture-MS Homo sapiens
41 LTV1  
Affinity Capture-MS Homo sapiens
42 C16orf72 29035
Affinity Capture-MS Homo sapiens
43 TRIP4 9325
Affinity Capture-MS Homo sapiens
44 CREB3L3  
Two-hybrid Homo sapiens
45 RPS7 6201
Affinity Capture-MS Homo sapiens
46 CASP10  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
47 OSBPL5 114879
Affinity Capture-MS Homo sapiens
48 NCL 4691
Affinity Capture-MS Homo sapiens
49 TM9SF4 9777
Affinity Capture-MS Homo sapiens
50 NOB1  
Affinity Capture-MS Homo sapiens
51 MAGEA6  
Affinity Capture-MS Homo sapiens
52 ASCC2 84164
Affinity Capture-MS Homo sapiens
53 CALM3 808
Affinity Capture-MS Homo sapiens
54 GNB2L1 10399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 RPS6 6194
Affinity Capture-MS Homo sapiens
56 XRCC3  
Affinity Capture-MS Homo sapiens
57 PSMC4 5704
Affinity Capture-MS Homo sapiens
58 TSR1 55720
Affinity Capture-MS Homo sapiens
59 SLC2A13 114134
Affinity Capture-MS Homo sapiens
60 RPS15A 6210
Affinity Capture-MS Homo sapiens
61 PSMB1 5689
Affinity Capture-MS Homo sapiens
62 BYSL 705
Affinity Capture-MS Homo sapiens
63 SEPT8 23176
Affinity Capture-MS Homo sapiens
64 RPS21 6227
Affinity Capture-MS Homo sapiens
65 TULP3 7289
Affinity Capture-MS Homo sapiens
66 RPS16 6217
Affinity Capture-MS Homo sapiens
67 RPS8 6202
Affinity Capture-MS Homo sapiens
68 RPS3 6188
Affinity Capture-MS Homo sapiens
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