Gene description for HCCS
Gene name holocytochrome c synthase
Gene symbol HCCS
Other names/aliases CCHL
LSDMCA1
MCOPS7
MLS
Species Homo sapiens
 Database cross references - HCCS
ExoCarta ExoCarta_3052
Vesiclepedia VP_3052
Entrez Gene 3052
HGNC 4837
MIM 300056
UniProt P53701  
 HCCS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HCCS
Molecular Function
    holocytochrome-c synthase activity GO:0004408 IBA
    holocytochrome-c synthase activity GO:0004408 IDA
    holocytochrome-c synthase activity GO:0004408 TAS
    protein binding GO:0005515 IPI
    heme binding GO:0020037 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    animal organ morphogenesis GO:0009887 TAS
    cytochrome c-heme linkage GO:0018063 IDA
    respiratory electron transport chain GO:0022904 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 TAS
    cytosol GO:0005829 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified HCCS in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HCCS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TADA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CLRN3 119467
Affinity Capture-MS Homo sapiens
3 MSRB2  
Co-fractionation Homo sapiens
4 HES3  
Affinity Capture-MS Homo sapiens
5 MRPL17  
Affinity Capture-MS Homo sapiens
6 MID1IP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KCNQ5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TYROBP  
Affinity Capture-MS Homo sapiens
10 LAMTOR2 28956
Co-fractionation Homo sapiens
11 IGHV1-46  
Affinity Capture-MS Homo sapiens
12 NDUFA4 4697
Affinity Capture-MS Homo sapiens
13 PEBP1 5037
Co-fractionation Homo sapiens
14 PLIN3 10226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ARL8B 55207
Affinity Capture-MS Homo sapiens
16 KIAA1429 25962
Affinity Capture-MS Homo sapiens
17 COQ9  
Affinity Capture-MS Homo sapiens
18 TIMMDC1  
Affinity Capture-MS Homo sapiens
19 FZR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZFYVE1 53349
Affinity Capture-MS Homo sapiens
21 ARMCX3 51566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FCRL4  
Affinity Capture-MS Homo sapiens
23 MRRF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 COMT 1312
Affinity Capture-MS Homo sapiens
26 EDAR  
Affinity Capture-MS Homo sapiens
27 GRPR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Wdr82  
Affinity Capture-MS Mus musculus
29 COX17  
Co-fractionation Homo sapiens
30 CPT2 1376
Co-fractionation Homo sapiens
31 APOO 79135
Proximity Label-MS Homo sapiens
32 PTGES2 80142
Affinity Capture-MS Homo sapiens
33 MTCH2 23788
Affinity Capture-MS Homo sapiens
34 MARCH7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RAB14 51552
Affinity Capture-MS Homo sapiens
36 TMX1 81542
Affinity Capture-MS Homo sapiens
37 CD7  
Affinity Capture-MS Homo sapiens
38 RAB21 23011
Affinity Capture-MS Homo sapiens
39 TXNRD3NB  
Affinity Capture-MS Homo sapiens
40 RRAS2 22800
Affinity Capture-MS Homo sapiens
41 ACACA 31
Positive Genetic Homo sapiens
42 ABHD11 83451
Co-fractionation Homo sapiens
43 TNFRSF9  
Affinity Capture-MS Homo sapiens
44 RDH11 51109
Affinity Capture-MS Homo sapiens
45 CYC1 1537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 LYPLA1 10434
Co-fractionation Homo sapiens
47 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 COX4I1 1327
Proximity Label-MS Homo sapiens
49 C15orf48  
Affinity Capture-MS Homo sapiens
50 ASCC3 10973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CHCHD5  
Affinity Capture-MS Homo sapiens
52 SLC25A12 8604
Proximity Label-MS Homo sapiens
53 AIFM1 9131
Proximity Label-MS Homo sapiens
54 SLC25A51  
Proximity Label-MS Homo sapiens
55 AGK 55750
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MGARP  
Affinity Capture-MS Homo sapiens
57 METTL16 79066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DCAKD 79877
Affinity Capture-MS Homo sapiens
59 CDC42 998
Co-fractionation Homo sapiens
60 IFNA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TXNDC12 51060
Co-fractionation Homo sapiens
62 MPZL1 9019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 MROH1 727957
Affinity Capture-MS Homo sapiens
64 TGFA  
Affinity Capture-MS Homo sapiens
65 GPATCH8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CERS6  
Affinity Capture-MS Homo sapiens
67 IGLC1 3537
Affinity Capture-MS Homo sapiens
68 IFNA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 COA4  
Co-fractionation Homo sapiens
70 AK4 205
Co-fractionation Homo sapiens
71 MTHFD2 10797
Co-fractionation Homo sapiens
72 PHB2 11331
Proximity Label-MS Homo sapiens
73 GGN  
Affinity Capture-MS Homo sapiens
74 ATL2 64225
Affinity Capture-MS Homo sapiens
75 RMND5B  
Affinity Capture-MS Homo sapiens
76 NPAT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 NSDHL 50814
Affinity Capture-MS Homo sapiens
78 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
79 GBA 2629
Affinity Capture-MS Homo sapiens
80 WIF1 11197
Affinity Capture-MS Homo sapiens
81 SFXN1 94081
Proximity Label-MS Homo sapiens
82 RAB2A 5862
Affinity Capture-MS Homo sapiens
83 SNX20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 C19orf52  
Proximity Label-MS Homo sapiens
85 POMK  
Affinity Capture-MS Homo sapiens
86 CYB5R3 1727
Co-fractionation Homo sapiens
87 PHF23 79142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 C1orf220  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 EVA1B 55194
Affinity Capture-MS Homo sapiens
90 CRTC3  
Affinity Capture-MS Homo sapiens
91 CYCS 54205
Affinity Capture-MS Homo sapiens
92 ESYT1 23344
Affinity Capture-MS Homo sapiens
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