Gene description for NSDHL
Gene name NAD(P) dependent steroid dehydrogenase-like
Gene symbol NSDHL
Other names/aliases H105E3
SDR31E1
XAP104
Species Homo sapiens
 Database cross references - NSDHL
ExoCarta ExoCarta_50814
Entrez Gene 50814
HGNC 13398
MIM 300275
UniProt Q15738  
 NSDHL identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for NSDHL
Molecular Function
    3-beta-hydroxy-delta5-steroid dehydrogenase activity GO:0003854 IEA
    sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity GO:0047012 IEA
Biological Process
    smoothened signaling pathway GO:0007224 IEA
    cholesterol biosynthetic process GO:0006695 TAS
    labyrinthine layer blood vessel development GO:0060716 IEA
    hair follicle development GO:0001942 IEA
    small molecule metabolic process GO:0044281 TAS
    oxidation-reduction process GO:0055114 IEA
Subcellular Localization
    lipid particle GO:0005811 IDA
    endoplasmic reticulum GO:0005783 IDA
    integral component of membrane GO:0016021 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified NSDHL in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NSDHL
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NSDHL is involved
PathwayEvidenceSource
Cholesterol biosynthesis TAS Reactome





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