Gene description for MTCL1
Gene name microtubule crosslinking factor 1
Gene symbol MTCL1
Other names/aliases CCDC165
KIAA0802
SOGA2
Species Homo sapiens
 Database cross references - MTCL1
ExoCarta ExoCarta_23255
Vesiclepedia VP_23255
Entrez Gene 23255
HGNC 29121
MIM 615766
UniProt Q9Y4B5  
 MTCL1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MTCL1
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    protein homodimerization activity GO:0042803 ISS
Biological Process
    microtubule bundle formation GO:0001578 IBA
    microtubule bundle formation GO:0001578 ISS
    chromosome segregation GO:0007059 IMP
    regulation of autophagy GO:0010506 IEA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IBA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISS
    cell division GO:0051301 IEA
    positive regulation of protein targeting to membrane GO:0090314 ISS
Subcellular Localization
    kinetochore GO:0000776 IDA
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IDA
    extracellular space GO:0005615 IEA
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IDA
    spindle microtubule GO:0005876 IDA
    apical plasma membrane GO:0016324 IEA
    apicolateral plasma membrane GO:0016327 IBA
    apicolateral plasma membrane GO:0016327 ISS
    lateral plasma membrane GO:0016328 IBA
    lateral plasma membrane GO:0016328 ISS
    midbody GO:0030496 IBA
    midbody GO:0030496 IDA
    microtubule bundle GO:0097427 IBA
    microtubule bundle GO:0097427 ISS
 Experiment description of studies that identified MTCL1 in sEVs
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MTCL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLNS1A 1207
Affinity Capture-MS Homo sapiens
2 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
3 MARK3 4140
Affinity Capture-MS Homo sapiens
4 SBDS 51119
Affinity Capture-MS Homo sapiens
5 MARK4  
Affinity Capture-MS Homo sapiens
6 P4HA3 283208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SENP3 26168
Affinity Capture-MS Homo sapiens
8 NSDHL 50814
Affinity Capture-MS Homo sapiens
9 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Set 56086
Affinity Capture-MS Mus musculus
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 PTMA 5757
Affinity Capture-MS Homo sapiens
14 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SAMHD1 25939
Affinity Capture-MS Homo sapiens
16 Dennd6a  
Affinity Capture-MS Mus musculus
17 DDX21 9188
Affinity Capture-MS Homo sapiens
18 KRT8 3856
Proximity Label-MS Homo sapiens
19 TOLLIP 54472
Proximity Label-MS Homo sapiens
20 Capza1 12340
Affinity Capture-MS Mus musculus
21 MEX3A  
Affinity Capture-RNA Homo sapiens
22 ZNF26  
Affinity Capture-MS Homo sapiens
23 RNF41  
Affinity Capture-MS Homo sapiens
24 TUBB3 10381
Reconstituted Complex Homo sapiens
25 Dlg5  
Affinity Capture-MS Mus musculus
26 RPS15 6209
Cross-Linking-MS (XL-MS) Homo sapiens
27 CAPZA1 829
Affinity Capture-MS Homo sapiens
28 Soga1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
29 PPP2R2C 5522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 WDR83  
Affinity Capture-MS Homo sapiens
31 MAGEA10  
Affinity Capture-MS Homo sapiens
32 METTL21B  
Affinity Capture-MS Homo sapiens
33 ZBTB1  
Affinity Capture-MS Homo sapiens
34 ZNF223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 Trip11  
Affinity Capture-MS Mus musculus
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 NDC80 10403
Proximity Label-MS Homo sapiens
38 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MARK2 2011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 TUBA1B 10376
Reconstituted Complex Homo sapiens
42 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 NEDD1 121441
Affinity Capture-MS Homo sapiens
44 KIF23 9493
Affinity Capture-MS Homo sapiens
45 GLI4  
Affinity Capture-MS Homo sapiens
46 PNMA2  
Affinity Capture-MS Homo sapiens
47 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ZNF555  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 FBXW7  
Affinity Capture-MS Homo sapiens
50 PRC1 9055
Affinity Capture-MS Homo sapiens
51 AMOT 154796
Proximity Label-MS Homo sapiens
52 MYO6 4646
Proximity Label-MS Homo sapiens
53 Dynll1 56455
Affinity Capture-MS Mus musculus
54 MTCL1 23255
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
55 DYNLL2 140735
Affinity Capture-MS Homo sapiens
56 PPP2R5A 5525
Affinity Capture-MS Homo sapiens
57 NR3C1 2908
Proximity Label-MS Homo sapiens
58 ANKRD44 91526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 Cenpe  
Affinity Capture-MS Mus musculus
60 SNX6 58533
Affinity Capture-MS Homo sapiens
61 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 C11orf68 83638
Two-hybrid Homo sapiens
63 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CDC5L 988
Affinity Capture-MS Homo sapiens
65 Mark2 13728
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which MTCL1 is involved
No pathways found





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