Gene description for NDC80
Gene name NDC80 kinetochore complex component
Gene symbol NDC80
Other names/aliases HEC
HEC1
HsHec1
KNTC2
TID3
hsNDC80
Species Homo sapiens
 Database cross references - NDC80
ExoCarta ExoCarta_10403
Vesiclepedia VP_10403
Entrez Gene 10403
HGNC 16909
MIM 607272
UniProt O14777  
 NDC80 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for NDC80
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
Biological Process
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IBA
    small GTPase mediated signal transduction GO:0007264 TAS
    mitotic spindle organization GO:0007052 IMP
    chromosome segregation GO:0007059 IMP
    establishment of mitotic spindle orientation GO:0000132 IMP
    attachment of spindle microtubules to kinetochore GO:0008608 IMP
    mitotic cell cycle GO:0000278 TAS
    cell division GO:0051301 IEA
    mitotic sister chromatid segregation GO:0000070 TAS
    mitotic nuclear division GO:0007067 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    condensed chromosome kinetochore GO:0000777 IDA
    nucleus GO:0005634 TAS
    condensed nuclear chromosome outer kinetochore GO:0000942 IDA
    condensed nuclear chromosome kinetochore GO:0000778 IBA
    membrane GO:0016020 IDA
    Ndc80 complex GO:0031262 IDA
    kinetochore GO:0000776 IDA
    chromosome, centromeric region GO:0000775 TAS
 Experiment description of studies that identified NDC80 in exosomes
1
Experiment ID 226
MISEV standards
EM
EV Biophysical techniques
GAPDH|CD9|FLOT1
EV Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for NDC80
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAD1L1  
Affinity Capture-MS Homo sapiens
2 RB1 5925
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 AMOTL2 51421
Two-hybrid Homo sapiens
4 MIS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SPC25 57405
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 SMC1A 8243
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 USHBP1  
Two-hybrid Homo sapiens
8 CCDC53 51019
Two-hybrid Homo sapiens
9 SPC24 147841
Affinity Capture-MS Homo sapiens
10 KLHL12  
Two-hybrid Homo sapiens
11 NEK2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 PLDN  
Two-hybrid Homo sapiens
13 CALCOCO1 57658
Two-hybrid Homo sapiens
14 MAD2L1 4085
Affinity Capture-MS Homo sapiens
15 TOM1L1 10040
Two-hybrid Homo sapiens
16 CDCA1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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