Gene description for IFT81
Gene name intraflagellar transport 81
Gene symbol IFT81
Other names/aliases CDV-1
CDV-1R
CDV1
CDV1R
DV1
Species Homo sapiens
 Database cross references - IFT81
ExoCarta ExoCarta_28981
Vesiclepedia VP_28981
Entrez Gene 28981
HGNC 14313
MIM 605489
UniProt Q8WYA0  
 IFT81 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT81
Molecular Function
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IBA
    tubulin binding GO:0015631 IDA
Biological Process
    spermatogenesis GO:0007283 ISS
    regulation of smoothened signaling pathway GO:0008589 IMP
    intraciliary anterograde transport GO:0035720 NAS
    intraciliary transport involved in cilium assembly GO:0035735 IMP
    intraciliary transport GO:0042073 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 NAS
    sperm flagellum assembly GO:0120316 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IEA
    centriole GO:0005814 IEA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle B GO:0030992 IBA
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    motile cilium GO:0031514 ISS
    ciliary basal body GO:0036064 IBA
    ciliary basal body GO:0036064 IDA
    sperm midpiece GO:0097225 IEA
    sperm principal piece GO:0097228 IEA
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified IFT81 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 IFT80 57560
Affinity Capture-MS Homo sapiens
3 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CCDC151  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 IFT27 11020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 WHAMMP3  
Affinity Capture-MS Homo sapiens
9 TTC26 79989
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCDC28B  
Affinity Capture-MS Homo sapiens
11 IFT20 90410
Affinity Capture-MS Homo sapiens
12 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NINL  
Proximity Label-MS Homo sapiens
14 UBXN10  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 C15orf59  
Affinity Capture-MS Homo sapiens
16 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 S100A2 6273
Affinity Capture-MS Homo sapiens
18 PLCD3 113026
Affinity Capture-MS Homo sapiens
19 PRAME  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 RPL21 6144
Cross-Linking-MS (XL-MS) Homo sapiens
23 GLI1  
Affinity Capture-MS Homo sapiens
24 CORO6  
Affinity Capture-MS Homo sapiens
25 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CEP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NDC80 10403
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ABTB2 25841
Affinity Capture-MS Homo sapiens
30 PRPF19 27339
Affinity Capture-MS Homo sapiens
31 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NIN 51199
Proximity Label-MS Homo sapiens
33 IFT74 80173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NLRP1  
Affinity Capture-MS Homo sapiens
37 TFPI2 7980
Affinity Capture-MS Homo sapiens
38 IFT52 51098
Affinity Capture-MS Homo sapiens
39 IFT88 8100
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
40 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 IFT172 26160
Affinity Capture-MS Homo sapiens
43 BICD1 636
Proximity Label-MS Homo sapiens
44 FEZF1  
Affinity Capture-MS Homo sapiens
45 PCM1 5108
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 POLR1E  
Affinity Capture-MS Homo sapiens
47 ARL13B 200894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 DUSP16  
Affinity Capture-MS Homo sapiens
49 ZC2HC1C  
Affinity Capture-MS Homo sapiens
50 APP 351
Reconstituted Complex Homo sapiens
51 TULP3 7289
Affinity Capture-MS Homo sapiens
52 CT45A5  
Affinity Capture-MS Homo sapiens
53 IFT22 64792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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