Gene description for IFT20
Gene name intraflagellar transport 20
Gene symbol IFT20
Other names/aliases -
Species Homo sapiens
 Database cross references - IFT20
ExoCarta ExoCarta_90410
Vesiclepedia VP_90410
Entrez Gene 90410
HGNC 30989
MIM 614394
UniProt Q8IY31  
 IFT20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IFT20
Molecular Function
    protein binding GO:0005515 IPI
    opsin binding GO:0002046 IEA
Biological Process
    regulation of autophagosome assembly GO:2000785 ISS
    smoothened signaling pathway GO:0007224 IEA
    regulation of canonical Wnt signaling pathway GO:0060828 IEA
    centrosome localization GO:0051642 IEA
    kidney development GO:0001822 IEA
    protein localization to cilium GO:0061512 IEA
    opsin transport GO:0036372 IEA
    neural precursor cell proliferation GO:0061351 IEA
    cilium assembly GO:0042384 IMP
    photoreceptor cell outer segment organization GO:0035845 IEA
    visual learning GO:0008542 IEA
    cardiac muscle cell differentiation GO:0055007 IEA
    regulation of cilium assembly GO:1902017 ISS
    organelle organization GO:0006996 TAS
    protein localization to Golgi apparatus GO:0034067 IMP
Subcellular Localization
    ciliary tip GO:0097542 TAS
    centriole GO:0005814 IEA
    primary cilium GO:0072372 TAS
    motile cilium GO:0031514 IEA
    centrosome GO:0005813 IDA
    ciliary base GO:0097546 IEA
    dendrite terminus GO:0044292 IEA
    microtubule cytoskeleton GO:0015630 IDA
    photoreceptor outer segment GO:0001750 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    extracellular exosome GO:0070062 IDA
    intraciliary transport particle B GO:0030992 ISS
    ciliary basal body GO:0036064 IEA
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified IFT20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IFT20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EXOC7 23265
Two-hybrid Homo sapiens
2 TREX1  
Two-hybrid Homo sapiens
3 ABI2 10152
Two-hybrid Homo sapiens
4 FBF1  
Two-hybrid Homo sapiens
5 KXD1 79036
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which IFT20 is involved
PathwayEvidenceSource
Intraflagellar transport TAS Reactome





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