Gene description for VPS18
Gene name vacuolar protein sorting 18 homolog (S. cerevisiae)
Gene symbol VPS18
Other names/aliases PEP3
Species Homo sapiens
 Database cross references - VPS18
ExoCarta ExoCarta_57617
Vesiclepedia VP_57617
Entrez Gene 57617
HGNC 15972
MIM 608551
UniProt Q9P253  
 VPS18 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for VPS18
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IDA
    protein-macromolecule adaptor activity GO:0030674 IBA
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IMP
Biological Process
    intracellular protein transport GO:0006886 IEA
    vesicle docking involved in exocytosis GO:0006904 IBA
    autophagy GO:0006914 IEA
    endosome organization GO:0007032 IBA
    endosome organization GO:0007032 IDA
    lysosome organization GO:0007040 IBA
    lysosome organization GO:0007040 IDA
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 IMP
    protein ubiquitination GO:0016567 IMP
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 IGI
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
    symbiont entry into host cell GO:0046718 IEA
    organelle fusion GO:0048284 IBA
    regulation of synaptic vesicle exocytosis GO:2000300 EXP
    regulation of synaptic vesicle exocytosis GO:2000300 IDA
    regulation of synaptic vesicle exocytosis GO:2000300 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IBA
    early endosome GO:0005769 IEA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    actin filament GO:0005884 IEA
    endosome membrane GO:0010008 TAS
    AP-3 adaptor complex GO:0030123 IEA
    clathrin-coated vesicle GO:0030136 IEA
    HOPS complex GO:0030897 IBA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 NAS
    late endosome membrane GO:0031902 IEA
    CORVET complex GO:0033263 NAS
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified VPS18 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 VPS41 27072
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 HOOK1  
Affinity Capture-Western Homo sapiens
4 FAM167A  
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 WHAMMP3  
Affinity Capture-MS Homo sapiens
7 LINC01565  
Affinity Capture-MS Homo sapiens
8 LAMP2 3920
Proximity Label-MS Homo sapiens
9 KIAA1429 25962
Affinity Capture-MS Homo sapiens
10 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 D2HGDH 728294
Affinity Capture-MS Homo sapiens
12 CT55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 IQGAP1 8826
Affinity Capture-MS Homo sapiens
14 STX7 8417
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 LSG1  
Affinity Capture-MS Homo sapiens
16 SEMA4C 54910
Affinity Capture-MS Homo sapiens
17 GGA1 26088
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 HYPM  
Affinity Capture-MS Homo sapiens
19 UTP20 27340
Co-fractionation Homo sapiens
20 NDUFV3 4731
Affinity Capture-MS Homo sapiens
21 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 VPS16 64601
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
23 AKTIP  
Affinity Capture-Western Homo sapiens
24 C9orf163  
Affinity Capture-MS Homo sapiens
25 LAMTOR1 55004
Proximity Label-MS Homo sapiens
26 IFT20 90410
Affinity Capture-MS Homo sapiens
27 Plk2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Biochemical Activity Rattus norvegicus
28 NF2 4771
Negative Genetic Homo sapiens
29 FAM96A  
Affinity Capture-MS Homo sapiens
30 SULT1C4  
Affinity Capture-MS Homo sapiens
31 B4GALT2  
Affinity Capture-MS Homo sapiens
32 RSRC1  
Affinity Capture-MS Homo sapiens
33 RAB4A 5867
Proximity Label-MS Homo sapiens
34 COPB1 1315
Two-hybrid Homo sapiens
35 C12orf49  
Negative Genetic Homo sapiens
36 VCP 7415
Affinity Capture-MS Homo sapiens
37 NTRK1 4914
Affinity Capture-MS Homo sapiens
38 RILP 83547
Reconstituted Complex Homo sapiens
39 MIA3 375056
Affinity Capture-MS Homo sapiens
40 BICD2 23299
Affinity Capture-MS Homo sapiens
41 KRT2 3849
Affinity Capture-MS Homo sapiens
42 DNAJB5  
Affinity Capture-MS Homo sapiens
43 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 BIN1 274
Affinity Capture-MS Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 UPP1 7378
Affinity Capture-MS Homo sapiens
47 DISC1 27185
Affinity Capture-MS Homo sapiens
48 TECPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 EFNA4  
Affinity Capture-MS Homo sapiens
51 ELMO3 79767
Affinity Capture-MS Homo sapiens
52 POLR3A 11128
Co-fractionation Homo sapiens
53 RAB5A 5868
Proximity Label-MS Homo sapiens
54 ZGRF1 55345
Affinity Capture-MS Homo sapiens
55 MON1B 22879
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
56 SYNPO2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
57 EGFR 1956
Negative Genetic Homo sapiens
58 DUSP16  
Affinity Capture-MS Homo sapiens
59 SERINC1 57515
Affinity Capture-MS Homo sapiens
60 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 CRYBB3  
Affinity Capture-MS Homo sapiens
62 UBE2D2 7322
Reconstituted Complex Homo sapiens
63 MAPK6  
Affinity Capture-MS Homo sapiens
64 C12orf74  
Affinity Capture-MS Homo sapiens
65 C3orf18  
Affinity Capture-MS Homo sapiens
66 SLC25A18  
Affinity Capture-MS Homo sapiens
67 RAB9A 9367
Proximity Label-MS Homo sapiens
68 OBSCN 84033
Affinity Capture-MS Homo sapiens
69 TJAP1 93643
Affinity Capture-MS Homo sapiens
70 PRSS1 5644
Affinity Capture-MS Homo sapiens
71 CCNA1  
Affinity Capture-MS Homo sapiens
72 SCLY  
Affinity Capture-MS Homo sapiens
73 STX17 55014
Affinity Capture-Western Homo sapiens
74 GGA3 23163
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
75 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
76 SRRM1 10250
Affinity Capture-MS Homo sapiens
77 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 MTA1 9112
Affinity Capture-MS Homo sapiens
79 EDEM1  
Affinity Capture-MS Homo sapiens
80 TSEN2  
Affinity Capture-MS Homo sapiens
81 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
82 DDX58 23586
Affinity Capture-RNA Homo sapiens
83 VPS33A 65082
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
84 DHFR 1719
Affinity Capture-MS Homo sapiens
85 KRT38 8687
Affinity Capture-MS Homo sapiens
86 CACNG8  
Affinity Capture-MS Homo sapiens
87 RAB7A 7879
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
88 RAB11A 8766
Proximity Label-MS Homo sapiens
89 EP300 2033
Two-hybrid Homo sapiens
90 ZUFSP 221302
Affinity Capture-MS Homo sapiens
91 GGA2 23062
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
92 KRAS 3845
Negative Genetic Homo sapiens
93 VPS11 55823
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 VPS39 23339
Affinity Capture-Western Homo sapiens
95 CCDC96  
Affinity Capture-MS Homo sapiens
96 Vps41  
Affinity Capture-MS Mus musculus
97 CCT8L2  
Affinity Capture-MS Homo sapiens
98 PMM1 5372
Affinity Capture-MS Homo sapiens
99 ALDOA 226
Affinity Capture-MS Homo sapiens
100 VPS8 23355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here