Gene description for MIA3
Gene name melanoma inhibitory activity family, member 3
Gene symbol MIA3
Other names/aliases ARNT
D320
TANGO
TANGO1
UNQ6077
Species Homo sapiens
 Database cross references - MIA3
ExoCarta ExoCarta_375056
Vesiclepedia VP_375056
Entrez Gene 375056
HGNC 24008
MIM 613455
UniProt Q5JRA6  
 MIA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MIA3
Molecular Function
    protein binding GO:0005515 IPI
    cargo receptor activity GO:0038024 IMP
Biological Process
    cell migration involved in sprouting angiogenesis GO:0002042 IMP
    positive regulation of leukocyte migration GO:0002687 IDA
    exocytosis GO:0006887 IMP
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    endoplasmic reticulum organization GO:0007029 IMP
    negative regulation of cell adhesion GO:0007162 IDA
    protein secretion GO:0009306 IBA
    protein transport GO:0015031 IMP
    negative regulation of cell migration GO:0030336 IDA
    vesicle cargo loading GO:0035459 IBA
    wound healing GO:0042060 IDA
    lipoprotein transport GO:0042953 IMP
    protein localization to endoplasmic reticulum exit site GO:0070973 IMP
    COPII-coated vesicle cargo loading GO:0090110 IMP
    cellular response to oxidised low-density lipoprotein particle stimulus GO:0140052 IMP
    negative regulation of leukocyte cell-cell adhesion GO:1903038 IMP
    negative regulation of lymphocyte migration GO:2000402 IMP
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    endoplasmic reticulum exit site GO:0070971 IBA
    endoplasmic reticulum exit site GO:0070971 IDA
 Experiment description of studies that identified MIA3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MIA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TTYH1  
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 CTAGE7P  
Affinity Capture-MS Homo sapiens
4 CTAGE8  
Cross-Linking-MS (XL-MS) Homo sapiens
5 OCLN 100506658
Affinity Capture-MS Homo sapiens
6 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 SYCE1  
Affinity Capture-MS Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CFC1  
Affinity Capture-MS Homo sapiens
12 NOP56 10528
Proximity Label-MS Homo sapiens
13 HSPA13 6782
Proximity Label-MS Homo sapiens
14 ECEL1  
Affinity Capture-MS Homo sapiens
15 HSD17B11 51170
Proximity Label-MS Homo sapiens
16 PXN 5829
Proximity Label-MS Homo sapiens
17 EYA2  
Proximity Label-MS Homo sapiens
18 DNAJC25 548645
Proximity Label-MS Homo sapiens
19 LRRC59 55379
Proximity Label-MS Homo sapiens
20 PTPN1 5770
Proximity Label-MS Homo sapiens
21 CLTB 1212
Affinity Capture-MS Homo sapiens
22 STX4 6810
Proximity Label-MS Homo sapiens
23 KIAA1429 25962
Affinity Capture-MS Homo sapiens
24 CLIC3 9022
Two-hybrid Homo sapiens
25 GXYLT1 283464
Affinity Capture-MS Homo sapiens
26 B3GAT1  
Proximity Label-MS Homo sapiens
27 SDF2L1 23753
Affinity Capture-MS Homo sapiens
28 NCR3  
Affinity Capture-MS Homo sapiens
29 SERPINA5 5104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SSR1 6745
Proximity Label-MS Homo sapiens
31 MARCH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RPN2 6185
Proximity Label-MS Homo sapiens
33 CTAGE6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DNAJC16  
Proximity Label-MS Homo sapiens
35 SLC25A46 91137
Proximity Label-MS Homo sapiens
36 P3H1 64175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GPRC5B 51704
Affinity Capture-MS Homo sapiens
38 CALR 811
Cross-Linking-MS (XL-MS) Homo sapiens
39 SIGLEC12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SEC23A 10484
Two-hybrid Homo sapiens
41 TMEM106A  
Affinity Capture-MS Homo sapiens
42 AMOT  
Proximity Label-MS Homo sapiens
43 CTAGE5 4253
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 SERPINA7 6906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 LMAN1 3998
Proximity Label-MS Homo sapiens
46 MTNR1B  
Two-hybrid Homo sapiens
47 NUP155 9631
Proximity Label-MS Homo sapiens
48 KIAA1715 80856
Proximity Label-MS Homo sapiens
49 PXMP2  
Proximity Label-MS Homo sapiens
50 KRT8 3856
Proximity Label-MS Homo sapiens
51 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 COLEC10 10584
Affinity Capture-MS Homo sapiens
53 ZNF444  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CEP55 55165
Two-hybrid Homo sapiens
55 EMD 2010
Proximity Label-MS Homo sapiens
56 QPRT 23475
Affinity Capture-MS Homo sapiens
57 ANO4 121601
Affinity Capture-MS Homo sapiens
58 RAB4A 5867
Proximity Label-MS Homo sapiens
59 DIABLO 56616
Proximity Label-MS Homo sapiens
60 MTMR7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 HAO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 NTRK1 4914
Affinity Capture-MS Homo sapiens
63 EEF1A1P5 158078
Cross-Linking-MS (XL-MS) Homo sapiens
64 DNAJB9 4189
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 STX6 10228
Proximity Label-MS Homo sapiens
66 RPN1 6184
Proximity Label-MS Homo sapiens
67 DNAJC30  
Proximity Label-MS Homo sapiens
68 LATS2 26524
Proximity Label-MS Homo sapiens
69 RHOT2 89941
Proximity Label-MS Homo sapiens
70 VPS18 57617
Affinity Capture-MS Homo sapiens
71 SEC61B 10952
Proximity Label-MS Homo sapiens
72 DNAJB11 51726
Proximity Label-MS Homo sapiens
73 RMDN3 55177
Proximity Label-MS Homo sapiens
74 FSTL1 11167
Cross-Linking-MS (XL-MS) Homo sapiens
75 REEP5 7905
Proximity Label-MS Homo sapiens
76 ZRANB1 54764
Affinity Capture-MS Homo sapiens
77 PRG2  
Affinity Capture-MS Homo sapiens
78 HSPA5 3309
Proximity Label-MS Homo sapiens
79 ANO6 196527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 CACNA1A  
Two-hybrid Homo sapiens
81 MINOS1  
Affinity Capture-MS Homo sapiens
82 MTMR14 64419
Affinity Capture-MS Homo sapiens
83 TPI1 7167
Cross-Linking-MS (XL-MS) Homo sapiens
84 SORT1 6272
Affinity Capture-MS Homo sapiens
85 DNAJB12 54788
Proximity Label-MS Homo sapiens
86 CALR3  
Proximity Label-MS Homo sapiens
87 PANX1 24145
Proximity Label-MS Homo sapiens
88 SLC39A5 283375
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 GGH 8836
Affinity Capture-MS Homo sapiens
90 APOB 338
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
91 BCAP31 10134
Proximity Label-MS Homo sapiens
92 HSD3B7 80270
Proximity Label-MS Homo sapiens
93 STIM1 6786
Proximity Label-MS Homo sapiens
94 FKBP8 23770
Proximity Label-MS Homo sapiens
95 PRTN3 5657
Affinity Capture-MS Homo sapiens
96 RIPK4  
Affinity Capture-MS Homo sapiens
97 Evpl  
Affinity Capture-MS Mus musculus
98 PTPRN  
Proximity Label-MS Homo sapiens
99 GPR114  
Affinity Capture-MS Homo sapiens
100 RPA3 6119
Proximity Label-MS Homo sapiens
101 NIN 51199
Proximity Label-MS Homo sapiens
102 ATP2A1 487
Proximity Label-MS Homo sapiens
103 FBXO2 26232
Affinity Capture-MS Homo sapiens
104 SAV1  
Proximity Label-MS Homo sapiens
105 CRTAP 10491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 RAB9A 9367
Proximity Label-MS Homo sapiens
107 TRIM27  
Two-hybrid Homo sapiens
108 ASPH 444
Affinity Capture-MS Homo sapiens
109 NDC80 10403
Proximity Label-MS Homo sapiens
110 DNAJC10 54431
Proximity Label-MS Homo sapiens
111 DNAJC3 5611
Proximity Label-MS Homo sapiens
112 NXPH3  
Affinity Capture-MS Homo sapiens
113 CYP2C9  
Proximity Label-MS Homo sapiens
114 AKAP1 8165
Proximity Label-MS Homo sapiens
115 TACC3  
Affinity Capture-MS Homo sapiens
116 BACE2 25825
Affinity Capture-MS Homo sapiens
117 ADAM30 11085
Affinity Capture-MS Homo sapiens
118 TMEM87A 25963
Affinity Capture-MS Homo sapiens
119 RNF43  
Proximity Label-MS Homo sapiens
120 SPCS3 60559
Affinity Capture-MS Homo sapiens
121 EDN3  
Affinity Capture-MS Homo sapiens
122 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 SIRT6  
Affinity Capture-MS Homo sapiens
124 SERPINA12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
126 GABRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 SIDT2 51092
Affinity Capture-MS Homo sapiens
128 PRDM14  
Two-hybrid Homo sapiens
129 UQCC1 55245
Affinity Capture-MS Homo sapiens
130 WWOX 51741
Affinity Capture-MS Homo sapiens
131 PDIA4 9601
Proximity Label-MS Homo sapiens
132 TALDO1 6888
Cross-Linking-MS (XL-MS) Homo sapiens
133 CALU 813
Proximity Label-MS Homo sapiens
134 SEC11A 23478
Affinity Capture-MS Homo sapiens
135 SLC39A7 7922
Affinity Capture-MS Homo sapiens
136 LENG8  
Two-hybrid Homo sapiens
137 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
138 SERPINA3 12
Affinity Capture-MS Homo sapiens
139 ERGIC1 57222
Proximity Label-MS Homo sapiens
140 MAP7D3 79649
Cross-Linking-MS (XL-MS) Homo sapiens
141 PPFIBP2 8495
Affinity Capture-MS Homo sapiens
142 LATS1  
Proximity Label-MS Homo sapiens
143 BRK1 55845
Affinity Capture-MS Homo sapiens
144 RAB5A 5868
Proximity Label-MS Homo sapiens
145 ERGIC2 51290
Proximity Label-MS Homo sapiens
146 SIRPD  
Affinity Capture-MS Homo sapiens
147 SEC62 7095
Proximity Label-MS Homo sapiens
148 SPCS2 9789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 CEP135  
Proximity Label-MS Homo sapiens
150 CLTA 1211
Proximity Label-MS Homo sapiens
151 HNRNPA1L2 144983
Affinity Capture-MS Homo sapiens
152 DNAJC1 64215
Proximity Label-MS Homo sapiens
153 SERPINA10 51156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
154 CKAP4 10970
Proximity Label-MS Homo sapiens
155 FXR2 9513
Two-hybrid Homo sapiens
156 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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