Gene description for CLIC3
Gene name chloride intracellular channel 3
Gene symbol CLIC3
Other names/aliases -
Species Homo sapiens
 Database cross references - CLIC3
ExoCarta ExoCarta_9022
Vesiclepedia VP_9022
Entrez Gene 9022
HGNC 2064
MIM 606533
UniProt O95833  
 CLIC3 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Urine 19056867    
 Gene ontology annotations for CLIC3
Molecular Function
    chloride channel activity GO:0005254 IBA
    chloride channel activity GO:0005254 IMP
    protein binding GO:0005515 IPI
    protein-disulfide reductase (glutathione) activity GO:0019153 IDA
Biological Process
    chloride transport GO:0006821 IBA
    chloride transport GO:0006821 IMP
    signal transduction GO:0007165 TAS
    chloride transmembrane transport GO:1902476 IEA
    positive regulation of sprouting angiogenesis GO:1903672 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IBA
    nuclear body GO:0016604 IDA
    extracellular matrix GO:0031012 IDA
    chloride channel complex GO:0034707 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CLIC3 in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CLIC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 KRT85  
Two-hybrid Homo sapiens
3 ALX1  
Two-hybrid Homo sapiens
4 TBC1D3  
Two-hybrid Homo sapiens
5 KATNBL1  
Two-hybrid Homo sapiens
6 EFHC2  
Two-hybrid Homo sapiens
7 C18orf21  
Affinity Capture-MS Homo sapiens
8 C9orf24  
Two-hybrid Homo sapiens
9 C11orf84  
Affinity Capture-MS Homo sapiens
10 POT1  
Two-hybrid Homo sapiens
11 SCML4  
Two-hybrid Homo sapiens
12 AGPAT1 10554
Affinity Capture-MS Homo sapiens
13 DDAH1 23576
Co-fractionation Homo sapiens
14 ANKRD55  
Two-hybrid Homo sapiens
15 PAN3 255967
Affinity Capture-MS Homo sapiens
16 AES 166
Two-hybrid Homo sapiens
17 CNOT4 4850
Affinity Capture-MS Homo sapiens
18 INCA1  
Two-hybrid Homo sapiens
19 PCDH12 51294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FKBP5 2289
Affinity Capture-MS Homo sapiens
21 SEPHS2  
Co-fractionation Homo sapiens
22 MIA2  
Affinity Capture-MS Homo sapiens
23 RFX1  
Affinity Capture-MS Homo sapiens
24 DDIT4L  
Two-hybrid Homo sapiens
25 MSX2  
Two-hybrid Homo sapiens
26 UBE2R2 54926
Affinity Capture-MS Homo sapiens
27 FNDC5  
Affinity Capture-MS Homo sapiens
28 STMN3  
Two-hybrid Homo sapiens
29 GDI2 2665
Co-fractionation Homo sapiens
30 ESR1  
Affinity Capture-MS Homo sapiens
31 C7orf31  
Two-hybrid Homo sapiens
32 HADH 3033
Co-fractionation Homo sapiens
33 PAN2  
Affinity Capture-MS Homo sapiens
34 MBNL1 4154
Affinity Capture-MS Homo sapiens
35 SSUH2  
Affinity Capture-MS Homo sapiens
36 ZNF620  
Two-hybrid Homo sapiens
37 TAGLN2 8407
Co-fractionation Homo sapiens
38 TBC1D3K  
Two-hybrid Homo sapiens
39 SARAF  
Affinity Capture-MS Homo sapiens
40 C11orf53  
Affinity Capture-MS Homo sapiens
41 FAM166B  
Two-hybrid Homo sapiens
42 GOT1 2805
Affinity Capture-MS Homo sapiens
43 TBC1D3C  
Two-hybrid Homo sapiens
44 MIA3 375056
Two-hybrid Homo sapiens
45 GLYR1 84656
Two-hybrid Homo sapiens
46 AGR2 10551
Two-hybrid Homo sapiens
47 LELP1  
Two-hybrid Homo sapiens
48 MBD3L1  
Two-hybrid Homo sapiens
49 KRTAP13-3  
Two-hybrid Homo sapiens
50 ZBTB42  
Two-hybrid Homo sapiens
51 ANKRD23 200539
Two-hybrid Homo sapiens
52 ZC2HC1C  
Two-hybrid Homo sapiens
53 CCR1  
Affinity Capture-MS Homo sapiens
54 ENTHD2  
Two-hybrid Homo sapiens
55 MAPK15  
Affinity Capture-Western Homo sapiens
56 AGBL3 340351
Affinity Capture-MS Homo sapiens
57 STX17 55014
Affinity Capture-MS Homo sapiens
58 IL1A  
Affinity Capture-MS Homo sapiens
59 VPS4A 27183
Two-hybrid Homo sapiens
60 PROSER2 254427
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLIC3 is involved
No pathways found





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