Gene description for CNOT4
Gene name CCR4-NOT transcription complex, subunit 4
Gene symbol CNOT4
Other names/aliases CLONE243
NOT4
NOT4H
Species Homo sapiens
 Database cross references - CNOT4
ExoCarta ExoCarta_4850
Entrez Gene 4850
HGNC 7880
MIM 604911
UniProt O95628  
 CNOT4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CNOT4
Molecular Function
    nucleotide binding GO:0000166 IEA
    ligase activity GO:0016874 IEA
    poly(A) RNA binding GO:0044822 IDA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    protein autoubiquitination GO:0051865 IDA
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 TAS
    gene expression GO:0010467 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
Subcellular Localization
    CCR4-NOT complex GO:0030014 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
 Experiment description of studies that identified CNOT4 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CNOT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT1 23019
Two-hybrid Homo sapiens
2 CNOT8  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CNOT4 is involved
PathwayEvidenceSource
Deadenylation of mRNA TAS Reactome





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