Gene description for RBM15
Gene name RNA binding motif protein 15
Gene symbol RBM15
Other names/aliases OTT
OTT1
SPEN
Species Homo sapiens
 Database cross references - RBM15
ExoCarta ExoCarta_64783
Entrez Gene 64783
HGNC 14959
MIM 606077
UniProt Q96T37  
 RBM15 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RBM15
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    positive regulation of transcription of Notch receptor target GO:0007221 IEA
    spleen development GO:0048536 IEA
    ventricular septum morphogenesis GO:0060412 IEA
    placenta blood vessel development GO:0060674 IEA
    negative regulation of myeloid cell differentiation GO:0045638 IEA
    patterning of blood vessels GO:0001569 IEA
    viral process GO:0016032 IEA
Subcellular Localization
    nuclear membrane GO:0031965 IEA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified RBM15 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RBM15
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RBM15 is involved
No pathways found





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