Experiment description of studies that identified RBM15 in exosomes

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for RBM15

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RIT1 6016 Negative Genetic Homo sapiens 2 SENP3 26168 Affinity Capture-MS Homo sapiens 3 RRN3   Co-fractionation Homo sapiens 4 COIL   Proximity Label-MS Homo sapiens 5 FGD2 221472 Affinity Capture-MS Homo sapiens 6 SRPK2 6733 Biochemical Activity Homo sapiens 7 RBM15B   Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 FGD1   Affinity Capture-MS Homo sapiens 9 YWHAE 7531 Affinity Capture-MS Homo sapiens 10 FGFBP1 9982 Affinity Capture-MS Homo sapiens 11 DDX17 10521 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 12 DHX8 1659 Proximity Label-MS Homo sapiens 13 NCKIPSD 51517 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 NAV2   Two-hybrid Homo sapiens 15 MUC20   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 WTAP 9589 Affinity Capture-MS Homo sapiens 17 DDX23 9416 Proximity Label-MS Homo sapiens 18 HDAC3 8841 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 19 PFKFB2 5208 Affinity Capture-MS Homo sapiens 20 SF3B1 23451 Affinity Capture-Western Homo sapiens 21 Srsf9   Two-hybrid Mus musculus 22 PRC1 9055 Affinity Capture-MS Homo sapiens 23 THTPA   Affinity Capture-MS Homo sapiens 24 KHDRBS1 10657 Co-fractionation Homo sapiens 25 CALCOCO2   Two-hybrid Homo sapiens 26 ABI1 10006 Two-hybrid Homo sapiens 27 OBSL1 23363 Affinity Capture-MS Homo sapiens 28 SRP14 6727 Affinity Capture-MS Homo sapiens 29 KRT31 3881 Two-hybrid Homo sapiens 30 EED   Affinity Capture-MS Homo sapiens 31 KLHL20   Affinity Capture-MS Homo sapiens 32 MECP2 4204 Affinity Capture-MS Homo sapiens 33 MYCN   Affinity Capture-MS Homo sapiens 34 SERBP1 26135 Affinity Capture-MS Homo sapiens 35 KIF14 9928 Affinity Capture-MS Homo sapiens 36 E2F4   Affinity Capture-MS Homo sapiens 37 DHX40   Proximity Label-MS Homo sapiens 38 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 39 TP53 7157 Affinity Capture-MS Homo sapiens 40 PMEL 6490 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 PARK2   Affinity Capture-MS Homo sapiens 42 CNOT4 4850 Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens Protein-peptide Homo sapiens 43 C12orf49   Negative Genetic Homo sapiens 44 RPA2 6118 Affinity Capture-MS Homo sapiens 45 NTRK1 4914 Affinity Capture-MS Homo sapiens 46 RAD18   Affinity Capture-MS Homo sapiens 47 ARRDC3 57561 Affinity Capture-MS Homo sapiens 48 HECTD1 25831 Affinity Capture-MS Homo sapiens 49 DDX39B 7919 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 50 LATS2 26524 Proximity Label-MS Homo sapiens 51 Cpsf6   Two-hybrid Mus musculus 52 CEBPA   Protein-peptide Homo sapiens 53 KDF1   Affinity Capture-MS Homo sapiens 54 HEATR6   Co-fractionation Homo sapiens 55 ANLN 54443 Affinity Capture-MS Homo sapiens 56 PHLPP1   Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 57 RNF40 9810 Affinity Capture-MS Homo sapiens 58 FANCD2   Affinity Capture-MS Homo sapiens 59 CLK3   Affinity Capture-MS Homo sapiens 60 RC3H2   Affinity Capture-MS Homo sapiens 61 DEPDC1B 55789 Affinity Capture-MS Homo sapiens 62 SIRT7   Affinity Capture-MS Homo sapiens 63 TSPYL2   Two-hybrid Homo sapiens 64 YWHAZ 7534 Affinity Capture-MS Homo sapiens 65 CTTN 2017 Two-hybrid Homo sapiens 66 EGFR 1956 Negative Genetic Homo sapiens 67 HNRNPA1 3178 Proximity Label-MS Homo sapiens 68 NXF1 10482 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 69 LZTS2 84445 Two-hybrid Homo sapiens 70 TPM1 7168 Co-fractionation Homo sapiens 71 MYC   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 72 RPA3 6119 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 73 ZC3H18   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 74 TOP1 7150 Affinity Capture-MS Homo sapiens 75 CUL7 9820 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 76 DDRGK1 65992 Affinity Capture-MS Homo sapiens 77 PEX3 8504 Proximity Label-MS Homo sapiens 78 DHX15 1665 Co-fractionation Homo sapiens 79 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 80 DIDO1   Co-fractionation Homo sapiens 81 Ewsr1 14030 Two-hybrid Mus musculus 82 CEP170P1   Affinity Capture-MS Homo sapiens 83 CPSF6 11052 Affinity Capture-MS Homo sapiens 84 TOR1AIP2 163590 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 85 VAV3 10451 Affinity Capture-MS Homo sapiens 86 RPA1 6117 Affinity Capture-MS Homo sapiens 87 SNRPA 6626 Affinity Capture-MS Homo sapiens 88 MAPK8 5599 Two-hybrid Homo sapiens 89 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 90 DDX58 23586 Affinity Capture-RNA Homo sapiens 91 WIF1 11197 Affinity Capture-MS Homo sapiens 92 UBE3C 9690 Co-fractionation Homo sapiens 93 CEP44   Two-hybrid Homo sapiens 94 UFL1 23376 Affinity Capture-MS Homo sapiens 95 Srsf1 110809 Affinity Capture-MS Mus musculus 96 ALYREF 10189 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 97 ARHGAP36   Affinity Capture-MS Homo sapiens 98 PINK1   Affinity Capture-MS Homo sapiens 99 METTL3   Affinity Capture-MS Homo sapiens 100 SOS2 6655 Affinity Capture-MS Homo sapiens 101 SNW1 22938 Affinity Capture-MS Homo sapiens 102 C9orf72   Affinity Capture-MS Homo sapiens 103 RC3H1 149041 Affinity Capture-MS Homo sapiens 104 SSRP1 6749 Affinity Capture-MS Homo sapiens 105 PRMT1 3276 Biochemical Activity Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 106 EP300 2033 Affinity Capture-MS Homo sapiens View the network

Pathways in which RBM15 is involved

No pathways found