Gene description for PEX3
Gene name peroxisomal biogenesis factor 3
Gene symbol PEX3
Other names/aliases PBD10A
TRG18
Species Homo sapiens
 Database cross references - PEX3
ExoCarta ExoCarta_8504
Vesiclepedia VP_8504
Entrez Gene 8504
HGNC 8858
MIM 603164
UniProt P56589  
 PEX3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for PEX3
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IDA
    protein-macromolecule adaptor activity GO:0030674 IBA
Biological Process
    peroxisome organization GO:0007031 IMP
    protein import into peroxisome membrane GO:0045046 IBA
    protein import into peroxisome membrane GO:0045046 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IMP
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 TAS
    endoplasmic reticulum GO:0005783 IDA
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    protein-lipid complex GO:0032994 IDA
 Experiment description of studies that identified PEX3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PEX3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANKRD13C  
Proximity Label-MS Homo sapiens
2 PEX13  
Proximity Label-MS Homo sapiens
3 SGCA  
Affinity Capture-MS Homo sapiens
4 AFG3L2 10939
Proximity Label-MS Homo sapiens
5 MON2 23041
Proximity Label-MS Homo sapiens
6 KLK15  
Affinity Capture-MS Homo sapiens
7 PSMD3 5709
Proximity Label-MS Homo sapiens
8 NUP188 23511
Proximity Label-MS Homo sapiens
9 PKP2 5318
Proximity Label-MS Homo sapiens
10 RAMP3  
Affinity Capture-MS Homo sapiens
11 TMEM135  
Proximity Label-MS Homo sapiens
12 MTPAP 55149
Proximity Label-MS Homo sapiens
13 SLC2A1 6513
Affinity Capture-MS Homo sapiens
14 SURF4 6836
Proximity Label-MS Homo sapiens
15 FPR1  
Affinity Capture-MS Homo sapiens
16 USP9X 8239
Proximity Label-MS Homo sapiens
17 SLC25A1 6576
Proximity Label-MS Homo sapiens
18 HNRNPR 10236
Co-fractionation Homo sapiens
19 TMEM72  
Affinity Capture-MS Homo sapiens
20 SNX6 58533
Proximity Label-MS Homo sapiens
21 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
22 MARC2 54996
Proximity Label-MS Homo sapiens
23 AKAP8L  
Proximity Label-MS Homo sapiens
24 LPCAT1 79888
Proximity Label-MS Homo sapiens
25 PEX1 5189
Proximity Label-MS Homo sapiens
26 ABCD1 215
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
27 LRPPRC 10128
Proximity Label-MS Homo sapiens
28 TIMMDC1  
Proximity Label-MS Homo sapiens
29 CHCHD4  
Co-fractionation Homo sapiens
30 DYNC1H1 1778
Proximity Label-MS Homo sapiens
31 FIS1 51024
Proximity Label-MS Homo sapiens
32 IARS2 55699
Proximity Label-MS Homo sapiens
33 MDN1 23195
Proximity Label-MS Homo sapiens
34 SMPD4 55627
Proximity Label-MS Homo sapiens
35 TELO2 9894
Proximity Label-MS Homo sapiens
36 SLC25A10 1468
Proximity Label-MS Homo sapiens
37 ABCD3 5825
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NUP93 9688
Proximity Label-MS Homo sapiens
39 MARCH5  
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Co-localization Homo sapiens
40 VPS13D 55187
Proximity Label-MS Homo sapiens
41 RPS9 6203
Proximity Label-MS Homo sapiens
42 XPO7 23039
Proximity Label-MS Homo sapiens
43 ATP2A3 489
Affinity Capture-MS Homo sapiens
44 SERPINH1 871
Proximity Label-MS Homo sapiens
45 EXD2  
Proximity Label-MS Homo sapiens
46 PHB 5245
Proximity Label-MS Homo sapiens
47 CALR 811
Proximity Label-MS Homo sapiens
48 MAVS 57506
Proximity Label-MS Homo sapiens
49 HSD17B4 3295
Proximity Label-MS Homo sapiens
50 ESYT2 57488
Proximity Label-MS Homo sapiens
51 FAF2 23197
Proximity Label-MS Homo sapiens
52 HK1 3098
Proximity Label-MS Homo sapiens
53 KIF14 9928
Affinity Capture-MS Homo sapiens
54 ATAD3A 55210
Proximity Label-MS Homo sapiens
55 PXMP2  
Proximity Label-MS Homo sapiens
56 XPO1 7514
Proximity Label-MS Homo sapiens
57 PDIA4 9601
Proximity Label-MS Homo sapiens
58 SNAP47 116841
Proximity Label-MS Homo sapiens
59 PPIB 5479
Proximity Label-MS Homo sapiens
60 PEX12  
Proximity Label-MS Homo sapiens
61 FASTKD5  
Proximity Label-MS Homo sapiens
62 CDC23 8697
Proximity Label-MS Homo sapiens
63 TNPO1 3842
Proximity Label-MS Homo sapiens
64 P2RY8  
Affinity Capture-MS Homo sapiens
65 ALPI  
Affinity Capture-MS Homo sapiens
66 LRRC25  
Affinity Capture-MS Homo sapiens
67 VRK1 7443
Proximity Label-MS Homo sapiens
68 UBAC2 337867
Proximity Label-MS Homo sapiens
69 NKRF 55922
Proximity Label-MS Homo sapiens
70 GPR182  
Affinity Capture-MS Homo sapiens
71 PLK1 5347
Proximity Label-MS Homo sapiens
72 PEX14 5195
Co-purification Homo sapiens
Proximity Label-MS Homo sapiens
73 P4HB 5034
Proximity Label-MS Homo sapiens
74 LMF2 91289
Proximity Label-MS Homo sapiens
75 RPN1 6184
Proximity Label-MS Homo sapiens
76 SMC1A 8243
Proximity Label-MS Homo sapiens
77 STX5 6811
Proximity Label-MS Homo sapiens
78 IARS 3376
Proximity Label-MS Homo sapiens
79 TMEM33 55161
Proximity Label-MS Homo sapiens
80 FLOT2 2319
Proximity Label-MS Homo sapiens
81 MFF 56947
Proximity Label-MS Homo sapiens
82 DNM1L 10059
Proximity Label-MS Homo sapiens
83 CDIPT 10423
Proximity Label-MS Homo sapiens
84 PEX6  
Proximity Label-MS Homo sapiens
85 IPO4 79711
Proximity Label-MS Homo sapiens
86 CYB5B 80777
Proximity Label-MS Homo sapiens
87 APLNR  
Affinity Capture-MS Homo sapiens
88 AIFM1 9131
Affinity Capture-MS Homo sapiens
89 CANX 821
Proximity Label-MS Homo sapiens
90 PRKCA 5578
Affinity Capture-MS Homo sapiens
91 DHCR7 1717
Proximity Label-MS Homo sapiens
92 DUOXA2  
Affinity Capture-MS Homo sapiens
93 ATP2B1 490
Proximity Label-MS Homo sapiens
94 LETM1 3954
Proximity Label-MS Homo sapiens
95 UQCRC2 7385
Proximity Label-MS Homo sapiens
96 CMKLR1 1240
Affinity Capture-MS Homo sapiens
97 EPPK1 83481
Proximity Label-MS Homo sapiens
98 MGARP  
Affinity Capture-MS Homo sapiens
99 RPL3 6122
Proximity Label-MS Homo sapiens
100 FKBP8 23770
Proximity Label-MS Homo sapiens
101 DHX30 22907
Proximity Label-MS Homo sapiens
102 AQP3  
Affinity Capture-MS Homo sapiens
103 OCIAD1 54940
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
104 NXF1 10482
Proximity Label-MS Homo sapiens
105 ACBD4  
Proximity Label-MS Homo sapiens
106 RPS27 6232
Proximity Label-MS Homo sapiens
107 BIRC6 57448
Proximity Label-MS Homo sapiens
108 KLRB1  
Affinity Capture-MS Homo sapiens
109 PEX16 9409
Proximity Label-MS Homo sapiens
110 PTPN1 5770
Proximity Label-MS Homo sapiens
111 FAR2  
Proximity Label-MS Homo sapiens
112 NCLN 56926
Proximity Label-MS Homo sapiens
113 DSTN 11034
Proximity Label-MS Homo sapiens
114 CLPTM1L 81037
Proximity Label-MS Homo sapiens
115 CLPB 81570
Proximity Label-MS Homo sapiens
116 ATP5C1 509
Proximity Label-MS Homo sapiens
117 PUS1 80324
Proximity Label-MS Homo sapiens
118 LMNA 4000
Proximity Label-MS Homo sapiens
119 FFAR1  
Affinity Capture-MS Homo sapiens
120 AKAP1 8165
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
121 CEPT1 10390
Proximity Label-MS Homo sapiens
122 HUWE1 10075
Proximity Label-MS Homo sapiens
123 ACBD5 91452
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
124 DHX15 1665
Co-fractionation Homo sapiens
125 MYO5A 4644
Proximity Label-MS Homo sapiens
126 SUN1 23353
Proximity Label-MS Homo sapiens
127 GLUD1 2746
Proximity Label-MS Homo sapiens
128 SLC25A11 8402
Proximity Label-MS Homo sapiens
129 ALDH3A2 224
Proximity Label-MS Homo sapiens
130 PEX11B 8799
Proximity Label-MS Homo sapiens
131 TDRKH  
Proximity Label-MS Homo sapiens
132 NUP205 23165
Proximity Label-MS Homo sapiens
133 CTDSP1 58190
Affinity Capture-MS Homo sapiens
134 UBXN4 23190
Proximity Label-MS Homo sapiens
135 TECR 9524
Proximity Label-MS Homo sapiens
136 TUBG1 7283
Proximity Label-MS Homo sapiens
137 PEX19 5824
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 LONP1 9361
Proximity Label-MS Homo sapiens
139 TRIP13 9319
Proximity Label-MS Homo sapiens
140 RPS4X 6191
Proximity Label-MS Homo sapiens
141 ITPR3 3710
Proximity Label-MS Homo sapiens
142 RBM15 64783
Proximity Label-MS Homo sapiens
143 SLC39A14 23516
Proximity Label-MS Homo sapiens
144 MFSD4  
Affinity Capture-MS Homo sapiens
145 ATP2A2 488
Proximity Label-MS Homo sapiens
146 UBE3C 9690
Proximity Label-MS Homo sapiens
147 FAR1 84188
Proximity Label-MS Homo sapiens
148 AUP1 550
Proximity Label-MS Homo sapiens
149 NAMPT 10135
Proximity Label-MS Homo sapiens
150 BRICD5  
Affinity Capture-MS Homo sapiens
151 BCL2  
Affinity Capture-MS Homo sapiens
152 SPPL2B 56928
Affinity Capture-MS Homo sapiens
153 RPL7A 6130
Proximity Label-MS Homo sapiens
154 YKT6 10652
Proximity Label-MS Homo sapiens
155 ARF1 375
Proximity Label-MS Homo sapiens
156 HNRNPF 3185
Co-fractionation Homo sapiens
157 PEX5 5830
Proximity Label-MS Homo sapiens
158 VIPR1  
Affinity Capture-MS Homo sapiens
159 SLC1A1 6505
Affinity Capture-MS Homo sapiens
160 USP30  
Proximity Label-MS Homo sapiens
161 PLD6  
Proximity Label-MS Homo sapiens
162 FPR2  
Affinity Capture-MS Homo sapiens
163 CDKN2A 1029
Proximity Label-MS Homo sapiens
164 DERL1 79139
Proximity Label-MS Homo sapiens
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