Gene description for ITPR3
Gene name inositol 1,4,5-trisphosphate receptor, type 3
Gene symbol ITPR3
Other names/aliases IP3R
IP3R3
Species Homo sapiens
 Database cross references - ITPR3
ExoCarta ExoCarta_3710
Entrez Gene 3710
HGNC 6182
MIM 147267
UniProt Q14573  
 ITPR3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for ITPR3
Molecular Function
    intracellular ligand-gated calcium channel activity GO:0005218 ISS
    inositol hexakisphosphate binding GO:0000822 ISS
    inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0005220 ISS
    inositol 1,3,4,5 tetrakisphosphate binding GO:0043533 ISS
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
    inositol 1,4,5 trisphosphate binding GO:0070679 IDA
Biological Process
    signal transduction GO:0007165 TAS
    inositol phosphate-mediated signaling GO:0048016 ISS
    innate immune response GO:0045087 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    calcium ion transport into cytosol GO:0060402 ISS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    small molecule metabolic process GO:0044281 TAS
    response to calcium ion GO:0051592 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    protein heterooligomerization GO:0051291 ISS
    sensory perception of umami taste GO:0050917 IEA
    platelet activation GO:0030168 TAS
    blood coagulation GO:0007596 TAS
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    memory GO:0007613 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    long-term synaptic potentiation GO:0060291 IEA
    G-protein coupled receptor signaling pathway GO:0007186 ISS
    calcium ion transmembrane transport GO:0070588 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    energy reserve metabolic process GO:0006112 TAS
    regulation of insulin secretion GO:0050796 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    sensory perception of bitter taste GO:0050913 IEA
    sensory perception of sweet taste GO:0050916 IEA
    protein homooligomerization GO:0051260 ISS
    activation of phospholipase C activity GO:0007202 TAS
Subcellular Localization
    brush border GO:0005903 ISS
    nuclear outer membrane GO:0005640 ISS
    receptor complex GO:0043235 IDA
    cytosol GO:0005829 ISS
    nucleolus GO:0005730 IEA
    nucleoplasm GO:0005654 IEA
    apical part of cell GO:0045177 ISS
    integral component of plasma membrane GO:0005887 IDA
    plasma membrane GO:0005886 IDA
    myelin sheath GO:0043209 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    endoplasmic reticulum GO:0005783 ISS
    platelet dense tubular network membrane GO:0031095 TAS
    cytoplasm GO:0005737 ISS
    neuronal cell body GO:0043025 ISS
    membrane GO:0016020 IDA
 Experiment description of studies that identified ITPR3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ITPR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITPR1  
Invivo Homo sapiens
2 TRPC5  
Reconstituted Complex Homo sapiens
3 TRPC2  
Reconstituted Complex Homo sapiens
4 TRPC6 7225
Reconstituted Complex Homo sapiens
5 TRPC1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 TRPC3  
Reconstituted Complex Homo sapiens
7 OPRS1  
Affinity Capture-Western Homo sapiens
8 CABP1 9478
Invivo Homo sapiens
9 TRPC7  
Reconstituted Complex Homo sapiens
10 TRPC4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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