Gene description for CHRNA3
Gene name cholinergic receptor, nicotinic, alpha 3 (neuronal)
Gene symbol CHRNA3
Other names/aliases LNCR2
NACHRA3
PAOD2
Species Homo sapiens
 Database cross references - CHRNA3
ExoCarta ExoCarta_1136
Entrez Gene 1136
HGNC 1957
MIM 118503
UniProt P32297  
 CHRNA3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CHRNA3
Molecular Function
    acetylcholine binding GO:0042166 IC
    acetylcholine-activated cation-selective channel activity GO:0004889 TAS
    acetylcholine receptor activity GO:0015464 IDA
    ligand-gated ion channel activity GO:0015276 TAS
Biological Process
    nervous system development GO:0007399 IMP
    synaptic transmission involved in micturition GO:0060084 IMP
    signal transduction GO:0007165 IDA
    synaptic transmission GO:0007268 TAS
    behavioral response to nicotine GO:0035095 IMP
    ion transmembrane transport GO:0034220 TAS
    regulation of dendrite morphogenesis GO:0048814 ISS
    cation transmembrane transport GO:0098655 TAS
    ion transport GO:0006811 NAS
    synaptic transmission, cholinergic GO:0007271 ISS
    regulation of acetylcholine secretion, neurotransmission GO:0014056 ISS
    activation of transmembrane receptor protein tyrosine kinase activity GO:0007171 ISS
    regulation of membrane potential GO:0042391 ISS
    locomotory behavior GO:0007626 ISS
    regulation of excitatory postsynaptic membrane potential GO:0060079 ISS
    regulation of smooth muscle contraction GO:0006940 ISS
Subcellular Localization
    cell junction GO:0030054 IEA
    postsynaptic density GO:0014069 ISS
    neuronal cell body GO:0043025 ISS
    integral component of membrane GO:0016021 NAS
    plasma membrane GO:0005886 TAS
    acetylcholine-gated channel complex GO:0005892 IDA
    dendrite GO:0030425 ISS
    postsynaptic membrane GO:0045211 IEA
 Experiment description of studies that identified CHRNA3 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CHRNA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHRNB4  
Invitro Homo sapiens
Invitro Homo sapiens
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