Gene description for CHRNA3
Gene name cholinergic receptor, nicotinic, alpha 3 (neuronal)
Gene symbol CHRNA3
Other names/aliases LNCR2
NACHRA3
PAOD2
Species Homo sapiens
 Database cross references - CHRNA3
ExoCarta ExoCarta_1136
Vesiclepedia VP_1136
Entrez Gene 1136
HGNC 1957
MIM 118503
UniProt P32297  
 CHRNA3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CHRNA3
Molecular Function
    protein binding GO:0005515 IPI
    ligand-gated monoatomic ion channel activity GO:0015276 TAS
    acetylcholine receptor activity GO:0015464 IDA
    acetylcholine-gated monoatomic cation-selective channel activity GO:0022848 IBA
    acetylcholine-gated monoatomic cation-selective channel activity GO:0022848 IDA
    acetylcholine-gated monoatomic cation-selective channel activity GO:0022848 ISS
    acetylcholine-gated monoatomic cation-selective channel activity GO:0022848 TAS
    acetylcholine binding GO:0042166 IC
Biological Process
    monoatomic ion transport GO:0006811 NAS
    regulation of smooth muscle contraction GO:0006940 ISS
    signal transduction GO:0007165 IDA
    activation of transmembrane receptor protein tyrosine kinase activity GO:0007171 ISS
    synaptic transmission, cholinergic GO:0007271 IBA
    synaptic transmission, cholinergic GO:0007271 ISS
    neuromuscular synaptic transmission GO:0007274 IBA
    nervous system development GO:0007399 IMP
    locomotory behavior GO:0007626 ISS
    regulation of acetylcholine secretion, neurotransmission GO:0014056 ISS
    monoatomic ion transmembrane transport GO:0034220 IBA
    monoatomic ion transmembrane transport GO:0034220 IEA
    response to nicotine GO:0035094 IBA
    behavioral response to nicotine GO:0035095 IMP
    regulation of membrane potential GO:0042391 ISS
    regulation of dendrite morphogenesis GO:0048814 ISS
    membrane depolarization GO:0051899 IBA
    excitatory postsynaptic potential GO:0060079 ISS
    synaptic transmission involved in micturition GO:0060084 IMP
    acetylcholine receptor signaling pathway GO:0095500 IBA
    acetylcholine receptor signaling pathway GO:0095500 ISS
    response to acetylcholine GO:1905144 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    acetylcholine-gated channel complex GO:0005892 IBA
    acetylcholine-gated channel complex GO:0005892 IDA
    postsynaptic density GO:0014069 ISS
    membrane GO:0016020 NAS
    nuclear speck GO:0016607 IDA
    dendrite GO:0030425 ISS
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 ISS
    plasma membrane raft GO:0044853 ISS
    synapse GO:0045202 IBA
    postsynaptic membrane GO:0045211 IEA
 Experiment description of studies that identified CHRNA3 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CHRNA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAM20B 9917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DOC2B  
Affinity Capture-MS Homo sapiens
4 ITPR2 3709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CLGN 1047
Affinity Capture-MS Homo sapiens
6 MFAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TMEM143  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KLC3  
Affinity Capture-MS Homo sapiens
9 Ubqln1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
10 CNNM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MIB1 57534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 EXTL2 2135
Affinity Capture-MS Homo sapiens
13 GHDC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GRAMD1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMEM219  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ITPR3 3710
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CHRNB2  
Affinity Capture-MS Homo sapiens
19 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 APOL2 23780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FAM69A 388650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 LEMD2 221496
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 FAR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TMPPE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TMEM231 79583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TPST2 8459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DSE 29940
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GLT8D2 83468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ST3GAL6 10402
Affinity Capture-MS Homo sapiens
31 GLRB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PLD6  
Affinity Capture-MS Homo sapiens
33 C1QL4  
Affinity Capture-MS Homo sapiens
34 CLRN2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here