Gene description for TPST2
Gene name tyrosylprotein sulfotransferase 2
Gene symbol TPST2
Other names/aliases TANGO13B
Species Homo sapiens
 Database cross references - TPST2
ExoCarta ExoCarta_8459
Vesiclepedia VP_8459
Entrez Gene 8459
HGNC 12021
MIM 603126
UniProt O60704  
 TPST2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TPST2
Molecular Function
    protein-tyrosine sulfotransferase activity GO:0008476 IBA
    protein-tyrosine sulfotransferase activity GO:0008476 IDA
    protein-tyrosine sulfotransferase activity GO:0008476 TAS
    protein homodimerization activity GO:0042803 IPI
Biological Process
    peptidyl-tyrosine sulfation GO:0006478 IDA
    3'-phosphoadenosine 5'-phosphosulfate metabolic process GO:0050427 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IBA
 Experiment description of studies that identified TPST2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TPST2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-MS Homo sapiens
2 MIS12  
Affinity Capture-MS Homo sapiens
3 NUF2  
Affinity Capture-MS Homo sapiens
4 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHRNA4  
Affinity Capture-MS Homo sapiens
6 TTYH3 80727
Affinity Capture-MS Homo sapiens
7 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NRSN1  
Affinity Capture-MS Homo sapiens
9 EIF2B2 8892
Affinity Capture-MS Homo sapiens
10 NSL1  
Affinity Capture-MS Homo sapiens
11 ATP12A 479
Affinity Capture-MS Homo sapiens
12 SETX  
Affinity Capture-MS Homo sapiens
13 CSE1L 1434
Affinity Capture-MS Homo sapiens
14 PMF1  
Affinity Capture-MS Homo sapiens
15 NUP160 23279
Affinity Capture-MS Homo sapiens
16 HEATR3 55027
Affinity Capture-MS Homo sapiens
17 RNF5  
Affinity Capture-MS Homo sapiens
18 TBC1D20  
Affinity Capture-MS Homo sapiens
19 USO1 8615
Affinity Capture-MS Homo sapiens
20 SPAG9 9043
Affinity Capture-MS Homo sapiens
21 DUSP14 11072
Affinity Capture-MS Homo sapiens
22 STX7 8417
Affinity Capture-MS Homo sapiens
23 KLRC1  
Affinity Capture-MS Homo sapiens
24 ATP2A3 489
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 IPO8 10526
Affinity Capture-MS Homo sapiens
26 C20orf24  
Affinity Capture-MS Homo sapiens
27 TOMM40 10452
Affinity Capture-MS Homo sapiens
28 DFNA5 1687
Affinity Capture-MS Homo sapiens
29 ZWINT  
Affinity Capture-MS Homo sapiens
30 TMEM106A  
Affinity Capture-MS Homo sapiens
31 ATP13A3 79572
Affinity Capture-MS Homo sapiens
32 TMEM59 9528
Affinity Capture-MS Homo sapiens
33 ATP1A3 478
Affinity Capture-MS Homo sapiens
34 CAMLG 819
Affinity Capture-MS Homo sapiens
35 HEATR1 55127
Affinity Capture-MS Homo sapiens
36 SLC30A1 7779
Affinity Capture-MS Homo sapiens
37 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 IPO7 10527
Affinity Capture-MS Homo sapiens
39 CLEC12B  
Affinity Capture-MS Homo sapiens
40 ADPGK 83440
Affinity Capture-MS Homo sapiens
41 MCOLN1 57192
Affinity Capture-MS Homo sapiens
42 TRAM1 23471
Affinity Capture-MS Homo sapiens
43 EIF2B5 8893
Affinity Capture-MS Homo sapiens
44 ATP2B1 490
Affinity Capture-MS Homo sapiens
45 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NOL11  
Affinity Capture-MS Homo sapiens
47 CHRNB2  
Affinity Capture-MS Homo sapiens
48 RAP1B 5908
Affinity Capture-MS Homo sapiens
49 PDS5A 23244
Affinity Capture-MS Homo sapiens
50 C1orf54  
Affinity Capture-MS Homo sapiens
51 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SLC7A2 6542
Affinity Capture-MS Homo sapiens
53 ATP2B4 493
Affinity Capture-MS Homo sapiens
54 MRAP2  
Affinity Capture-MS Homo sapiens
55 ZYG11B  
Affinity Capture-MS Homo sapiens
56 TMCO3 55002
Affinity Capture-MS Homo sapiens
57 EIF2B4 8890
Affinity Capture-MS Homo sapiens
58 TMED1 11018
Affinity Capture-MS Homo sapiens
59 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 IPO9 55705
Affinity Capture-MS Homo sapiens
61 DSN1 79980
Affinity Capture-MS Homo sapiens
62 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PLAA 9373
Affinity Capture-MS Homo sapiens
64 YIPF6 286451
Affinity Capture-MS Homo sapiens
65 POTEI 653269
Affinity Capture-MS Homo sapiens
66 SMIM5  
Affinity Capture-MS Homo sapiens
67 SPC24 147841
Affinity Capture-MS Homo sapiens
68 ERLEC1 27248
Affinity Capture-MS Homo sapiens
69 RMND1 55005
Affinity Capture-MS Homo sapiens
70 TNPO3 23534
Affinity Capture-MS Homo sapiens
71 ATXN10 25814
Affinity Capture-MS Homo sapiens
72 SLC7A3  
Affinity Capture-MS Homo sapiens
73 KIAA1524 57650
Affinity Capture-MS Homo sapiens
74 EIF2B3 8891
Affinity Capture-MS Homo sapiens
75 TMEM169  
Affinity Capture-MS Homo sapiens
76 XPO6 23214
Affinity Capture-MS Homo sapiens
77 SAAL1 113174
Affinity Capture-MS Homo sapiens
78 XPO4 64328
Affinity Capture-MS Homo sapiens
79 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
80 CST4 1472
Affinity Capture-MS Homo sapiens
81 GBF1 8729
Affinity Capture-MS Homo sapiens
82 CITED2  
Affinity Capture-MS Homo sapiens
83 SCGB2A2  
Affinity Capture-MS Homo sapiens
84 NDC80 10403
Affinity Capture-MS Homo sapiens
85 KDM8  
Affinity Capture-MS Homo sapiens
86 DYM  
Affinity Capture-MS Homo sapiens
87 THADA 63892
Affinity Capture-MS Homo sapiens
88 LARP4 113251
Affinity Capture-MS Homo sapiens
89 ATP2B3 492
Affinity Capture-MS Homo sapiens
90 KLHL13 90293
Affinity Capture-MS Homo sapiens
91 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
92 VAC14 55697
Affinity Capture-MS Homo sapiens
93 GPR180  
Affinity Capture-MS Homo sapiens
94 LRRC55  
Affinity Capture-MS Homo sapiens
95 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
96 INTS2  
Affinity Capture-MS Homo sapiens
97 PEX19 5824
Affinity Capture-MS Homo sapiens
98 NUP98 4928
Affinity Capture-MS Homo sapiens
99 SCN4A  
Affinity Capture-MS Homo sapiens
100 NF1 4763
Affinity Capture-MS Homo sapiens
101 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
102 NOC2L 26155
Affinity Capture-MS Homo sapiens
103 TPST1 8460
Affinity Capture-MS Homo sapiens
104 SEC62 7095
Affinity Capture-MS Homo sapiens
105 IPO4 79711
Affinity Capture-MS Homo sapiens
106 ATP2B2 491
Affinity Capture-MS Homo sapiens
107 INTS3 65123
Affinity Capture-MS Homo sapiens
108 CHRNE  
Affinity Capture-MS Homo sapiens
109 SLC7A1 6541
Affinity Capture-MS Homo sapiens
110 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 CHRNA3 1136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 ATP13A1 57130
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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