Gene description for CST4
Gene name cystatin S
Gene symbol CST4
Other names/aliases -
Species Homo sapiens
 Database cross references - CST4
ExoCarta ExoCarta_1472
Vesiclepedia VP_1472
Entrez Gene 1472
HGNC 2476
MIM 123857
UniProt P01036  
 CST4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for CST4
Molecular Function
    cysteine-type endopeptidase inhibitor activity GO:0004869 IBA
    cysteine-type endopeptidase inhibitor activity GO:0004869 IDA
    protein binding GO:0005515 IPI
Biological Process
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IDA
    negative regulation of proteolysis GO:0045861 IDA
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IBA
    vesicle GO:0031982 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CST4 in exosomes
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CST4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLEKHG6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RING1 6015
Affinity Capture-MS Homo sapiens
3 LYPD6B 130576
Affinity Capture-MS Homo sapiens
4 CST1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TMEM176B 28959
Affinity Capture-MS Homo sapiens
6 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DCTN3 11258
Affinity Capture-MS Homo sapiens
8 CHAC2 494143
Affinity Capture-MS Homo sapiens
9 TSFM 10102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CRH  
Affinity Capture-MS Homo sapiens
11 ZNHIT2 741
Affinity Capture-MS Homo sapiens
12 SNAPC1 6617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 AKNAD1  
Affinity Capture-MS Homo sapiens
14 ABCA8 10351
Affinity Capture-MS Homo sapiens
15 ZG16B 124220
Affinity Capture-MS Homo sapiens
16 COMMD1 150684
Affinity Capture-MS Homo sapiens
17 CRYGN  
Affinity Capture-MS Homo sapiens
18 TCF23  
Affinity Capture-MS Homo sapiens
19 IGLL5 100423062
Affinity Capture-MS Homo sapiens
20 KCNG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ACTR3 10096
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CCRL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PMEPA1 56937
Affinity Capture-MS Homo sapiens
24 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DISP1 84976
Affinity Capture-MS Homo sapiens
26 CMKLR1 1240
Affinity Capture-MS Homo sapiens
27 SLC25A10 1468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TSGA10IP  
Affinity Capture-MS Homo sapiens
29 UCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SLIRP 81892
Affinity Capture-MS Homo sapiens
31 UBQLN2 29978
Two-hybrid Homo sapiens
32 TADA3  
Affinity Capture-MS Homo sapiens
33 COG2 22796
Affinity Capture-MS Homo sapiens
34 RNF141  
Affinity Capture-MS Homo sapiens
35 FTH1 2495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CORO1C 23603
Affinity Capture-MS Homo sapiens
38 GTF2B 2959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 FKBP14  
Affinity Capture-MS Homo sapiens
40 HBQ1  
Affinity Capture-MS Homo sapiens
41 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
42 EPSTI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 STK11 6794
Affinity Capture-MS Homo sapiens
44 DAZ2  
Affinity Capture-MS Homo sapiens
45 KIR2DS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FKBP5 2289
Affinity Capture-MS Homo sapiens
47 GJB5  
Affinity Capture-MS Homo sapiens
48 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MUC5AC 4586
Affinity Capture-MS Homo sapiens
50 RCAN1  
Affinity Capture-MS Homo sapiens
51 ZNF491  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 UBTD2 92181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ECE2  
Affinity Capture-MS Homo sapiens
54 SCAMP2 10066
Affinity Capture-MS Homo sapiens
55 CHST7  
Affinity Capture-MS Homo sapiens
56 TTL 150465
Affinity Capture-MS Homo sapiens
57 CAMK1D 57118
Affinity Capture-MS Homo sapiens
58 HBM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RHOBTB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 GKN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 LRRC8E 80131
Affinity Capture-MS Homo sapiens
62 GIMAP1  
Affinity Capture-MS Homo sapiens
63 TPST2 8459
Affinity Capture-MS Homo sapiens
64 C10orf53  
Affinity Capture-MS Homo sapiens
65 POLL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ATP11C 286410
Affinity Capture-MS Homo sapiens
67 RCAN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HINT2 84681
Affinity Capture-MS Homo sapiens
69 CFB 629
Affinity Capture-MS Homo sapiens
70 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 UQCRQ 27089
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 NPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RNF208  
Affinity Capture-MS Homo sapiens
74 NXT1  
Affinity Capture-MS Homo sapiens
75 KLK10 5655
Affinity Capture-MS Homo sapiens
76 APLF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 TIMM10  
Affinity Capture-MS Homo sapiens
78 B3GALNT1 8706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ITLN2  
Affinity Capture-MS Homo sapiens
80 PRKCE 5581
Affinity Capture-MS Homo sapiens
81 CUL4A 8451
Affinity Capture-MS Homo sapiens
82 GREM2 64388
Affinity Capture-MS Homo sapiens
83 NKAP 79576
Affinity Capture-MS Homo sapiens
84 SRRT 51593
Affinity Capture-MS Homo sapiens
85 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CST4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here