Gene description for MUC5AC
Gene name mucin 5AC, oligomeric mucus/gel-forming
Gene symbol MUC5AC
Other names/aliases MUC5
TBM
leB
mucin
Species Homo sapiens
 Database cross references - MUC5AC
ExoCarta ExoCarta_4586
Vesiclepedia VP_4586
Entrez Gene 4586
HGNC 7515
MIM 158373
 MUC5AC identified in sEVs derived from the following tissue/cell type
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Hepatocellular carcinoma cells 26054723    
Saliva 19199708    
 Gene ontology annotations for MUC5AC
Molecular Function
    extracellular matrix structural constituent GO:0005201 TAS
    metal ion binding GO:0046872 IEA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    extracellular matrix GO:0031012 IBA
    extracellular exosome GO:0070062 HDA
    mucus layer GO:0070701 IDA
 Experiment description of studies that identified MUC5AC in sEVs
1
Experiment ID 924
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 927
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|SDCBP|CD81|RAB35|LAMP2|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for MUC5AC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FNDC5  
Affinity Capture-MS Homo sapiens
2 FKBP14  
Affinity Capture-MS Homo sapiens
3 GALNT12 79695
Biochemical Activity Homo sapiens
4 RIBC1  
Affinity Capture-MS Homo sapiens
5 GNG8  
Affinity Capture-MS Homo sapiens
6 GAN 8139
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 CST4 1472
Affinity Capture-MS Homo sapiens
9 TWF1 5756
Affinity Capture-MS Homo sapiens
10 IGHA2 3494
Affinity Capture-MS Homo sapiens
11 KLK10 5655
Affinity Capture-MS Homo sapiens
12 GSK3B 2932
Affinity Capture-MS Homo sapiens
13 RASL11B  
Affinity Capture-MS Homo sapiens
14 SLC5A1 6523
Affinity Capture-MS Homo sapiens
15 HBM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 GALNT14  
Biochemical Activity Homo sapiens
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