Gene description for MUC5AC
Gene name mucin 5AC, oligomeric mucus/gel-forming
Gene symbol MUC5AC
Other names/aliases MUC5
TBM
leB
mucin
Species Homo sapiens
 Database cross references - MUC5AC
ExoCarta ExoCarta_4586
Entrez Gene 4586
HGNC 7515
MIM 158373
 MUC5AC identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Saliva 19199708    
 Gene ontology annotations for MUC5AC
Molecular Function
    extracellular matrix structural constituent GO:0005201 TAS
Biological Process
    protein O-linked glycosylation GO:0006493 TAS
    cellular protein metabolic process GO:0044267 TAS
    phosphatidylinositol-mediated signaling GO:0048015 IDA
    post-translational protein modification GO:0043687 TAS
    extracellular fibril organization GO:0043206 IDA
    O-glycan processing GO:0016266 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    Golgi lumen GO:0005796 TAS
    fibril GO:0043205 IDA
 Experiment description of studies that identified MUC5AC in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for MUC5AC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GALNT12  
Biochemical Activity Homo sapiens
2 GALNT14  
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which MUC5AC is involved
No pathways found





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