Gene description for TWF1
Gene name twinfilin actin binding protein 1
Gene symbol TWF1
Other names/aliases A6
PTK9
Species Homo sapiens
 Database cross references - TWF1
ExoCarta ExoCarta_5756
Entrez Gene 5756
HGNC 9620
MIM 610932
UniProt Q12792  
 TWF1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for TWF1
Molecular Function
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    actin monomer binding GO:0003785 ISS
    protein tyrosine kinase activity GO:0004713 IDA
    ATP binding GO:0005524 IDA
Biological Process
    negative regulation of actin filament polymerization GO:0030837 ISS
    regulation of actin phosphorylation GO:0043538 IDA
    sequestering of actin monomers GO:0042989 ISS
Subcellular Localization
    focal adhesion GO:0005925 IDA
    ruffle membrane GO:0032587 ISS
    myofibril GO:0030016 ISS
    filopodium GO:0030175 ISS
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    cell-cell junction GO:0005911 ISS
    actin cytoskeleton GO:0015629 ISS
 Experiment description of studies that identified TWF1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TWF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TWF1 is involved
No pathways found





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