Gene description for TIGAR
Gene name TP53 induced glycolysis regulatory phosphatase
Gene symbol TIGAR
Other names/aliases C12orf5
FR2BP
Species Homo sapiens
 Database cross references - TIGAR
ExoCarta ExoCarta_57103
Vesiclepedia VP_57103
Entrez Gene 57103
HGNC 1185
MIM 610775
UniProt Q9NQ88  
 TIGAR identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TIGAR
Molecular Function
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 EXP
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 IBA
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 IDA
    protein binding GO:0005515 IPI
Biological Process
    mitophagy GO:0000423 IEA
    response to ischemia GO:0002931 ISS
    fructose 2,6-bisphosphate metabolic process GO:0006003 IEA
    glycolytic process GO:0006096 IEA
    DNA damage response GO:0006974 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to gamma radiation GO:0010332 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    positive regulation of cardiac muscle cell apoptotic process GO:0010666 IEA
    glucose catabolic process to lactate via pyruvate GO:0019661 IEA
    negative regulation of programmed cell death GO:0043069 IDA
    regulation of pentose-phosphate shunt GO:0043456 IBA
    regulation of pentose-phosphate shunt GO:0043456 IMP
    positive regulation of DNA repair GO:0045739 IMP
    negative regulation of glycolytic process GO:0045820 IBA
    intestinal epithelial cell development GO:0060576 IEA
    cellular response to cobalt ion GO:0071279 IDA
    cellular response to hypoxia GO:0071456 IDA
    reactive oxygen species metabolic process GO:0072593 IEA
    negative regulation of mitophagy GO:1901525 IEA
    regulation of response to DNA damage checkpoint signaling GO:1902153 IMP
    positive regulation of hexokinase activity GO:1903301 IDA
    negative regulation of glucose catabolic process to lactate via pyruvate GO:1904024 IEA
    positive regulation of pentose-phosphate shunt GO:1905857 IEA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial outer membrane GO:0005741 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TIGAR in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TIGAR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Co-fractionation Homo sapiens
2 RPL11 6135
Two-hybrid Homo sapiens
3 TXN2 25828
Co-fractionation Homo sapiens
4 GSX1  
Affinity Capture-MS Homo sapiens
5 LYRM2  
Co-fractionation Homo sapiens
6 HSPA5 3309
Proximity Label-MS Homo sapiens
7 PNPLA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TWF1 5756
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ETFA 2108
Co-fractionation Homo sapiens
10 MTPN 136319
Affinity Capture-MS Homo sapiens
11 NMI  
Affinity Capture-MS Homo sapiens
12 TSC2 7249
Co-fractionation Homo sapiens
13 AGPAT1 10554
Affinity Capture-MS Homo sapiens
14 RPA2 6118
Proximity Label-MS Homo sapiens
15 UBE2L3 7332
Co-fractionation Homo sapiens
16 ANXA1 301
Co-fractionation Homo sapiens
17 NEUROG3  
Affinity Capture-MS Homo sapiens
18 NTNG1  
Affinity Capture-MS Homo sapiens
19 FAM184A 79632
Proximity Label-MS Homo sapiens
20 TP53 7157
Co-localization Homo sapiens
21 DNAJB4 11080
Proximity Label-MS Homo sapiens
22 IGHG2 3501
Affinity Capture-MS Homo sapiens
23 TKT 7086
Co-fractionation Homo sapiens
24 NUP35 129401
Proximity Label-MS Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 ALG8  
Affinity Capture-MS Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 PCBP1 5093
Co-fractionation Homo sapiens
29 GUK1 2987
Co-fractionation Homo sapiens
30 SET 6418
Co-localization Homo sapiens
31 PIGT 51604
Affinity Capture-MS Homo sapiens
32 TRIM35 23087
Affinity Capture-Western Homo sapiens
33 MSRB3 253827
Co-fractionation Homo sapiens
34 HMGCL 3155
Co-fractionation Homo sapiens
35 KLK13  
Affinity Capture-MS Homo sapiens
36 TBCA 6902
Co-fractionation Homo sapiens
37 CCR1  
Affinity Capture-MS Homo sapiens
38 HINT1 3094
Co-fractionation Homo sapiens
39 PHF11 51131
Affinity Capture-MS Homo sapiens
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