Gene description for GUK1
Gene name guanylate kinase 1
Gene symbol GUK1
Other names/aliases GMK
Species Homo sapiens
 Database cross references - GUK1
ExoCarta ExoCarta_2987
Vesiclepedia VP_2987
Entrez Gene 2987
HGNC 4693
MIM 139270
UniProt Q16774  
 GUK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GUK1
Molecular Function
    guanylate kinase activity GO:0004385 EXP
    guanylate kinase activity GO:0004385 IBA
    guanylate kinase activity GO:0004385 IDA
    guanylate kinase activity GO:0004385 IMP
    guanylate kinase activity GO:0004385 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
Biological Process
    purine nucleotide metabolic process GO:0006163 IDA
    dGDP biosynthetic process GO:0006185 IMP
    xenobiotic metabolic process GO:0006805 IDA
    xenobiotic metabolic process GO:0006805 TAS
    nucleobase-containing small molecule interconversion GO:0015949 TAS
    GDP-mannose metabolic process GO:0019673 IEA
    glycoprotein transport GO:0034436 IEA
    GMP metabolic process GO:0046037 IEA
    dGMP metabolic process GO:0046054 IEA
    dATP metabolic process GO:0046060 IEA
    GDP biosynthetic process GO:0046711 IEA
Subcellular Localization
    photoreceptor inner segment GO:0001917 ISS
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GUK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GUK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GLRX5 51218
Co-fractionation Homo sapiens
2 TXN2 25828
Co-fractionation Homo sapiens
3 AK4 205
Co-fractionation Homo sapiens
4 ACOT7 11332
Co-fractionation Homo sapiens
5 LYRM2  
Co-fractionation Homo sapiens
6 HNRNPD 3184
Two-hybrid Homo sapiens
7 PRDX5 25824
Co-fractionation Homo sapiens
8 PRDX6 9588
Co-fractionation Homo sapiens
9 PSAP 5660
Co-fractionation Homo sapiens
10 AK2 204
Co-fractionation Homo sapiens
11 BAX 581
Co-fractionation Homo sapiens
12 MTPN 136319
Co-fractionation Homo sapiens
13 C2orf68  
Affinity Capture-MS Homo sapiens
14 ISCA2 122961
Co-fractionation Homo sapiens
15 MCEMP1  
Affinity Capture-MS Homo sapiens
16 LAP3 51056
Co-fractionation Homo sapiens
17 IQCB1  
Affinity Capture-MS Homo sapiens
18 NLGN3  
Two-hybrid Homo sapiens
19 ANXA1 301
Co-fractionation Homo sapiens
20 COX5A 9377
Co-fractionation Homo sapiens
21 GABARAPL2 11345
Co-fractionation Homo sapiens
22 TIGAR 57103
Co-fractionation Homo sapiens
23 EGFR 1956
Negative Genetic Homo sapiens
24 FAHD1 81889
Affinity Capture-MS Homo sapiens
25 VHL  
Two-hybrid Homo sapiens
26 C15orf54  
Affinity Capture-MS Homo sapiens
27 NDUFA4 4697
Affinity Capture-MS Homo sapiens
28 NUDT19 390916
Co-fractionation Homo sapiens
29 PUF60 22827
Co-fractionation Homo sapiens
30 COA7  
Co-fractionation Homo sapiens
31 NXF1 10482
Affinity Capture-RNA Homo sapiens
32 DPY19L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TXN 7295
Co-fractionation Homo sapiens
34 TTC19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MOV10 4343
Affinity Capture-RNA Homo sapiens
36 TXNDC12 51060
Co-fractionation Homo sapiens
37 ABHD11 83451
Co-fractionation Homo sapiens
38 PMPCA 23203
Co-fractionation Homo sapiens
39 ESD 2098
Co-fractionation Homo sapiens
40 LACTB2 51110
Co-fractionation Homo sapiens
41 GSTK1 373156
Co-fractionation Homo sapiens
42 MSRB3 253827
Co-fractionation Homo sapiens
43 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
44 TCOF1 6949
Co-fractionation Homo sapiens
45 DPY19L2P4  
Affinity Capture-MS Homo sapiens
46 TBCA 6902
Co-fractionation Homo sapiens
47 FXN  
Co-fractionation Homo sapiens
48 HINT1 3094
Co-fractionation Homo sapiens
49 CLIC4 25932
Co-fractionation Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 TMEM97 27346
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SRI 6717
Co-fractionation Homo sapiens
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