Gene description for GUK1
Gene name guanylate kinase 1
Gene symbol GUK1
Other names/aliases GMK
Species Homo sapiens
 Database cross references - GUK1
ExoCarta ExoCarta_2987
Entrez Gene 2987
HGNC 4693
MIM 139270
UniProt Q16774  
 GUK1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GUK1
Molecular Function
    ATP binding GO:0005524 IEA
    guanylate kinase activity GO:0004385 IDA
Biological Process
    nucleobase-containing small molecule interconversion GO:0015949 TAS
    GDP-mannose metabolic process GO:0019673 IEA
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    glycoprotein transport GO:0034436 IEA
    ATP metabolic process GO:0046034 IEA
    nucleotide phosphorylation GO:0046939 IEA
    drug metabolic process GO:0017144 TAS
    small molecule metabolic process GO:0044281 TAS
    GMP metabolic process GO:0046037 IEA
    dGMP metabolic process GO:0046054 IEA
    GDP biosynthetic process GO:0046711 IEA
    dATP metabolic process GO:0046060 IEA
    dGDP biosynthetic process GO:0006185 IEA
    purine nucleotide metabolic process GO:0006163 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GUK1 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GUK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GUK1 is involved
PathwayEvidenceSource
Abacavir metabolism TAS Reactome
Synthesis and interconversion of nucleotide di- and triphosphates TAS Reactome





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