Gene description for ISCA2
Gene name iron-sulfur cluster assembly 2
Gene symbol ISCA2
Other names/aliases HBLD1
ISA2
c14_5557
Species Homo sapiens
 Database cross references - ISCA2
ExoCarta ExoCarta_122961
Vesiclepedia VP_122961
Entrez Gene 122961
HGNC 19857
MIM 615317
UniProt Q86U28  
 ISCA2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ISCA2
Molecular Function
    iron ion binding GO:0005506 IBA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    2 iron, 2 sulfur cluster binding GO:0051537 IBA
    4 iron, 4 sulfur cluster binding GO:0051539 IBA
Biological Process
    iron-sulfur cluster assembly GO:0016226 IBA
    protein maturation by [4Fe-4S] cluster transfer GO:0106035 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 TAS
    mitochondrial [4Fe-4S] assembly complex GO:0120510 IDA
 Experiment description of studies that identified ISCA2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ISCA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AK4 205
Co-fractionation Homo sapiens
2 ZMYM6  
Affinity Capture-MS Homo sapiens
3 SIKE1  
Affinity Capture-MS Homo sapiens
4 ISCA1  
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 GUCD1  
Two-hybrid Homo sapiens
7 DPP9 91039
Affinity Capture-MS Homo sapiens
8 PSAP 5660
Co-fractionation Homo sapiens
9 GUK1 2987
Co-fractionation Homo sapiens
10 GLRX5 51218
Co-fractionation Homo sapiens
11 HBS1L 10767
Affinity Capture-MS Homo sapiens
12 MTPN 136319
Co-fractionation Homo sapiens
13 GAR1 54433
Affinity Capture-MS Homo sapiens
14 PRNP 5621
Affinity Capture-MS Homo sapiens
15 AKT1 207
Biochemical Activity Homo sapiens
16 ARHGEF11 9826
Affinity Capture-MS Homo sapiens
17 LONRF2  
Affinity Capture-MS Homo sapiens
18 STRN4 29888
Affinity Capture-MS Homo sapiens
19 HEPHL1  
Affinity Capture-MS Homo sapiens
20 STRIP1 85369
Affinity Capture-MS Homo sapiens
21 LRRC15 131578
Affinity Capture-MS Homo sapiens
22 RNF41  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
23 GABARAPL2 11345
Co-fractionation Homo sapiens
24 STRN3 29966
Affinity Capture-MS Homo sapiens
25 VSIG8  
Affinity Capture-MS Homo sapiens
26 NPPA  
Affinity Capture-MS Homo sapiens
27 WDYHV1  
Two-hybrid Homo sapiens
28 EGFR 1956
Negative Genetic Homo sapiens
29 S100A3  
Affinity Capture-MS Homo sapiens
30 MOB4 25843
Affinity Capture-MS Homo sapiens
31 CALML3 810
Affinity Capture-MS Homo sapiens
32 FAM122A  
Affinity Capture-MS Homo sapiens
33 SLMAP 7871
Affinity Capture-MS Homo sapiens
34 COA7  
Co-fractionation Homo sapiens
35 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
36 DUSP13  
Affinity Capture-MS Homo sapiens
37 SPINK5 11005
Affinity Capture-MS Homo sapiens
38 NARF 26502
Affinity Capture-MS Homo sapiens
39 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
40 PLEKHG4  
Affinity Capture-MS Homo sapiens
41 ACAD9 28976
Affinity Capture-MS Homo sapiens
42 GEM  
Two-hybrid Homo sapiens
43 STRN 6801
Affinity Capture-MS Homo sapiens
44 FGFR1OP2  
Affinity Capture-MS Homo sapiens
45 SERPINC1 462
Affinity Capture-MS Homo sapiens
46 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 DBT 1629
Affinity Capture-MS Homo sapiens
48 OSBPL9 114883
Affinity Capture-MS Homo sapiens
49 CRYAB 1410
Affinity Capture-MS Homo sapiens
50 LACTB2 51110
Co-fractionation Homo sapiens
51 MSRB3 253827
Co-fractionation Homo sapiens
52 THNSL1 79896
Affinity Capture-MS Homo sapiens
53 PPP2CA 5515
Affinity Capture-MS Homo sapiens
54 DUSP14 11072
Affinity Capture-MS Homo sapiens
55 POLA1  
Affinity Capture-MS Homo sapiens
56 AIM1 202
Affinity Capture-MS Homo sapiens
57 IBA57  
Affinity Capture-MS Homo sapiens
58 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
59 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
60 TBCA 6902
Co-fractionation Homo sapiens
61 RAB11FIP5 26056
Affinity Capture-MS Homo sapiens
62 FXN  
Co-fractionation Homo sapiens
63 FAM124B  
Two-hybrid Homo sapiens
64 UBE2O 63893
Affinity Capture-MS Homo sapiens
65 ENTHD2  
Affinity Capture-MS Homo sapiens
66 EIF3E 3646
Two-hybrid Homo sapiens
67 DSG4 147409
Affinity Capture-MS Homo sapiens
68 COX4I1 1327
Co-fractionation Homo sapiens
69 SRI 6717
Co-fractionation Homo sapiens
70 SUOX  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here