ExoCarta: Gene summary

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Gene description for DBT
Gene name	dihydrolipoamide branched chain transacylase E2
Gene symbol	DBT
Other names/aliases	BCATE2 BCKAD-E2 BCKADE2 BCOADC-E2 E2 E2B
Species	Homo sapiens
Database cross references - DBT
ExoCarta	ExoCarta_1629
Vesiclepedia	VP_1629
Entrez Gene	1629
HGNC	2698
MIM	248610
UniProt	P11182
DBT identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for DBT

Molecular Function
	protein binding	GO:0005515	IPI
	acetyltransferase activity	GO:0016407	IBA
	acyltransferase activity, transferring groups other than amino-acyl groups	GO:0016747	TAS
	lipoic acid binding	GO:0031405	IBA
	ubiquitin protein ligase binding	GO:0031625	IPI
	dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity	GO:0043754	IEA
	branched-chain alpha-keto acid dehydrogenase activity	GO:0047101	IDA
Biological Process
	branched-chain amino acid catabolic process	GO:0009083	IDA
	branched-chain amino acid catabolic process	GO:0009083	TAS
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IBA
	mitochondrion	GO:0005739	IDA
	mitochondrial matrix	GO:0005759	NAS
	mitochondrial matrix	GO:0005759	TAS
	cytosol	GO:0005829	IDA
	microtubule cytoskeleton	GO:0015630	IDA
	mitochondrial nucleoid	GO:0042645	IDA
	oxoglutarate dehydrogenase complex	GO:0045252	TAS
	branched-chain alpha-ketoacid dehydrogenase complex	GO:0160157	IPI

Experiment description of studies that identified DBT in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for DBT

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

SLFN11

91607

Proximity Label-MS

Homo sapiens

SLIRP

81892

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HOOK1

Proximity Label-MS

Homo sapiens

SPRTN

Affinity Capture-MS

Homo sapiens

MSRB2

Affinity Capture-MS

Homo sapiens

OXLD1

Affinity Capture-MS

Homo sapiens

FASTKD5

Proximity Label-MS

Homo sapiens

EPB41L5

57669

Affinity Capture-MS

Homo sapiens

MTG2

Proximity Label-MS

Homo sapiens

DYNC1I1

Proximity Label-MS

Homo sapiens

TRIM33

51592

Affinity Capture-MS

Homo sapiens

USP16

Affinity Capture-MS

Homo sapiens

RPUSD4

84881

Proximity Label-MS

Homo sapiens

MTRF1

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GFM2

84340

Proximity Label-MS

Homo sapiens

CCDC90B

Proximity Label-MS

Homo sapiens

LRPPRC

10128

Proximity Label-MS

Homo sapiens

PPTC7

160760

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

IGF2BP3

10643

Affinity Capture-MS

Homo sapiens

TFAM

7019

Proximity Label-MS

Homo sapiens

GATM

Affinity Capture-MS

Homo sapiens

PMPCA

23203

Proximity Label-MS

Homo sapiens

PPP6C

5537

Affinity Capture-MS

Homo sapiens

PPM1K

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HINT2

84681

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AGO3

Affinity Capture-MS

Homo sapiens

SRRM2

23524

Affinity Capture-MS

Homo sapiens

BRPF3

Affinity Capture-MS

Homo sapiens

MDH2

4191

Proximity Label-MS

Homo sapiens

C21orf33

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRDM4

Affinity Capture-MS

Homo sapiens

MRPS26

64949

Proximity Label-MS

Homo sapiens

EXD2

Proximity Label-MS

Homo sapiens

ATG16L1

55054

Affinity Capture-MS

Homo sapiens

FAHD2B

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

PMPCB

9512

Proximity Label-MS

Homo sapiens

TACO1

Proximity Label-MS

Homo sapiens

TNFRSF1B

Affinity Capture-MS

Homo sapiens

MTERF3

Proximity Label-MS

Homo sapiens

DYNLT1

6993

Proximity Label-MS

Homo sapiens

DDX58

23586

Affinity Capture-RNA

Homo sapiens

ZADH2

284273

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NF2

4771

Affinity Capture-MS

Homo sapiens

RAB17

64284

Affinity Capture-MS

Homo sapiens

DLD

1738

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C6orf203

Proximity Label-MS

Homo sapiens

THEM4

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ADI1

55256

Affinity Capture-MS

Homo sapiens

PARK2

Affinity Capture-MS

Homo sapiens

LIPT1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPS11

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TUFM

7284

Proximity Label-MS

Homo sapiens

ACOT13

Affinity Capture-MS

Homo sapiens

MMAB

326625

Affinity Capture-MS

Homo sapiens

COX8A

Proximity Label-MS

Homo sapiens

OGT

8473

Reconstituted Complex

Homo sapiens

CECR2

Affinity Capture-MS

Homo sapiens

CCDC109B

55013

Proximity Label-MS

Homo sapiens

C9orf170

Affinity Capture-MS

Homo sapiens

TBRG4

9238

Proximity Label-MS

Homo sapiens

FAM133A

286499

Affinity Capture-MS

Homo sapiens

2271

Affinity Capture-MS

Homo sapiens

HMGCL

3155

Affinity Capture-MS

Homo sapiens

TRUB2

Proximity Label-MS

Homo sapiens

CA5B

11238

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPL11

65003

Proximity Label-MS

Homo sapiens

MTRF1L

Proximity Label-MS

Homo sapiens

FAHD2A

51011

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SP100

6672

Affinity Capture-MS

Homo sapiens

COX4I1

1327

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ECH1

1891

Affinity Capture-MS

Homo sapiens

MRM1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RBM5

Affinity Capture-MS

Homo sapiens

COX5B

1329

Affinity Capture-MS

Homo sapiens

MRRF

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AIFM1

9131

Proximity Label-MS

Homo sapiens

MTIF2

4528

Proximity Label-MS

Homo sapiens

BCKDHB

594

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NUDT6

Affinity Capture-MS

Homo sapiens

MRPS6

Affinity Capture-MS

Homo sapiens

RALB

5899

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTIF3

Proximity Label-MS

Homo sapiens

TAL1

Proximity Label-MS

Homo sapiens

SURF6

Affinity Capture-MS

Homo sapiens

PHIP

55023

Affinity Capture-MS

Homo sapiens

CDO1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NR3C1

2908

Proximity Label-MS

Homo sapiens

ECHDC2

55268

Affinity Capture-MS

Homo sapiens

FAHD1

81889

Affinity Capture-MS

Homo sapiens

HSD17B10

3028

Affinity Capture-MS

Homo sapiens

ECHS1

1892

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BRD1

23774

Affinity Capture-MS

Homo sapiens

C12orf65

Proximity Label-MS

Homo sapiens

AGO4

Affinity Capture-MS

Homo sapiens

MYO6

4646

Proximity Label-MS

Homo sapiens

MYC

Affinity Capture-MS

Homo sapiens

PCBD2

84105

Affinity Capture-MS

Homo sapiens

DLST

1743

Co-fractionation

Homo sapiens

100

ABCC1

4363

Co-fractionation

Homo sapiens

101

ACAD9

28976

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

102

BICD1

636

Proximity Label-MS

Homo sapiens

103

MRPS36

92259

Affinity Capture-MS

Homo sapiens

104

DNAJB13

Affinity Capture-MS

Homo sapiens

105

FOXQ1

Proximity Label-MS

Homo sapiens

106

BKRF1

Affinity Capture-MS

107

HOGA1

Affinity Capture-MS

Homo sapiens

108

PDP1

54704

Affinity Capture-MS

Homo sapiens

109

METTL15

Proximity Label-MS

Homo sapiens

110

MRPL18

29074

Affinity Capture-MS

Homo sapiens

111

HUWE1

10075

Affinity Capture-MS

Homo sapiens

112

MECR

51102

Affinity Capture-MS

Homo sapiens

113

HECTD1

25831

Affinity Capture-MS

Homo sapiens

114

CLPP

8192

Proximity Label-MS

Homo sapiens

115

PPT1

5538

Affinity Capture-MS

Homo sapiens

116

1431

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

117

C17orf80

55028

Proximity Label-MS

Homo sapiens

118

GRPEL2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

119

TSFM

10102

Proximity Label-MS

Homo sapiens

120

AUH

549

Proximity Label-MS

Homo sapiens

121

IDH3B

3420

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

122

MCAT

27349

Affinity Capture-MS

Homo sapiens

123

ICT1

3396

Proximity Label-MS

Homo sapiens

124

ISCA2

122961

Affinity Capture-MS

Homo sapiens

125

HDAC6

10013

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

126

MRPS18C

Affinity Capture-MS

Homo sapiens

127

MRPL53

Affinity Capture-MS

Homo sapiens

128

ACAA2

10449

Affinity Capture-MS

Homo sapiens

129

PDHA1

5160

Proximity Label-MS

Homo sapiens

130

AGMAT

Affinity Capture-MS

Homo sapiens

131

TRMT61B

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

132

DCAF11

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

133

Hdac6

Affinity Capture-MS

Mus musculus

134

NIPSNAP3B

55335

Affinity Capture-MS

Homo sapiens

135

SSBP1

6742

Proximity Label-MS

Homo sapiens

136

FASTKD3

Proximity Label-MS

Homo sapiens

137

C10orf88

Affinity Capture-MS

Homo sapiens

138

GFM1

85476

Proximity Label-MS

Homo sapiens

139

AARS2

Proximity Label-MS

Homo sapiens

140

TRIP4

9325

Affinity Capture-MS

Homo sapiens

141

DYNLRB1

83658

Proximity Label-MS

Homo sapiens

142

RPUSD3

Proximity Label-MS

Homo sapiens

143

GRSF1

2926

Proximity Label-MS

Homo sapiens

144

MICU3

Affinity Capture-MS

Homo sapiens

145

METTL17

Proximity Label-MS

Homo sapiens

146

ATXN3

4287

Affinity Capture-MS

Homo sapiens

147

MCEE

84693

Affinity Capture-MS

Homo sapiens

148

RBCK1

Affinity Capture-MS

Homo sapiens

149

NDUFS6

Affinity Capture-MS

Homo sapiens

150

IFIT2

3433

Affinity Capture-MS

Homo sapiens

151

CCRN4L

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which DBT is involved

Pathway	Evidence	Source
BCKDH synthesizes BCAA-CoA from KIC, KMVA, KIV	TAS	Reactome
Branched-chain amino acid catabolism	TAS	Reactome
Branched-chain ketoacid dehydrogenase kinase deficiency	TAS	Reactome
Disease	TAS	Reactome
Diseases of branched-chain amino acid catabolism	TAS	Reactome
Diseases of metabolism	TAS	Reactome
H139Hfs13* PPM1K causes a mild variant of MSUD	TAS	Reactome
Loss-of-function mutations in BCKDHA or BCKDHB cause MSUD	TAS	Reactome
Loss-of-function mutations in DBT cause MSUD2	TAS	Reactome
Loss-of-function mutations in DLD cause MSUD3/DLDD	TAS	Reactome
Maple Syrup Urine Disease	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of amino acids and derivatives	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Mitochondrial protein degradation	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Protein lipoylation	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RHOH GTPase cycle	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

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