Gene description for COX5B
Gene name cytochrome c oxidase subunit Vb
Gene symbol COX5B
Other names/aliases COXVB
Species Homo sapiens
 Database cross references - COX5B
ExoCarta ExoCarta_1329
Entrez Gene 1329
HGNC 2269
MIM 123866
UniProt P10606  
 COX5B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for COX5B
Molecular Function
    protein binding GO:0005515 IPI
    cytochrome-c oxidase activity GO:0004129 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    hydrogen ion transmembrane transport GO:1902600 TAS
    respiratory gaseous exchange GO:0007585 TAS
    gene expression GO:0010467 TAS
    respiratory electron transport chain GO:0022904 TAS
    cellular metabolic process GO:0044237 TAS
Subcellular Localization
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified COX5B in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for COX5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NELFCD 51497
Affinity Capture-MS Homo sapiens
2 AR 367
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which COX5B is involved
PathwayEvidenceSource
Respiratory electron transport TAS Reactome
TP53 Regulates Metabolic Genes TAS Reactome





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