Gene description for COA3
Gene name cytochrome c oxidase assembly factor 3
Gene symbol COA3
Other names/aliases CCDC56
HSPC009
MITRAC12
Species Homo sapiens
 Database cross references - COA3
ExoCarta ExoCarta_28958
Vesiclepedia VP_28958
Entrez Gene 28958
HGNC 24990
MIM 614775
UniProt Q9Y2R0  
 COA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for COA3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial cytochrome c oxidase assembly GO:0033617 IBA
    mitochondrial cytochrome c oxidase assembly GO:0033617 IMP
    positive regulation of mitochondrial translation GO:0070131 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified COA3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for COA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
2 PHB 5245
Co-fractionation Homo sapiens
3 TOMM40 10452
Co-fractionation Homo sapiens
4 PDHA1 5160
Co-fractionation Homo sapiens
5 CXCL2 2920
Affinity Capture-MS Homo sapiens
6 ATP5H 10476
Co-fractionation Homo sapiens
7 SLIRP 81892
Affinity Capture-Western Homo sapiens
8 C15orf48  
Affinity Capture-MS Homo sapiens
9 SLC25A24 29957
Co-fractionation Homo sapiens
10 PHB2 11331
Co-fractionation Homo sapiens
11 VDAC3 7419
Co-fractionation Homo sapiens
12 HADHB 3032
Co-fractionation Homo sapiens
13 HADHA 3030
Co-fractionation Homo sapiens
14 VAPB 9217
Co-fractionation Homo sapiens
15 SNX25 83891
Affinity Capture-MS Homo sapiens
16 CANX 821
Co-fractionation Homo sapiens
17 NDUFS1 4719
Co-fractionation Homo sapiens
18 NDUFB10 4716
Co-fractionation Homo sapiens
19 COX4I2 84701
Affinity Capture-Western Homo sapiens
20 NDUFA9 4704
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
21 PCCB 5096
Co-fractionation Homo sapiens
22 NDUFB1 4707
Co-fractionation Homo sapiens
23 COX4I1 1327
Affinity Capture-Western Homo sapiens
24 VDAC2 7417
Co-fractionation Homo sapiens
25 TUFM 7284
Affinity Capture-Western Homo sapiens
26 UQCRC2 7385
Co-fractionation Homo sapiens
27 COX14  
Affinity Capture-MS Homo sapiens
28 KEAP1 9817
Negative Genetic Homo sapiens
29 COX7A2 1347
Co-fractionation Homo sapiens
30 COX5A 9377
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
31 DNAJA3 9093
Proximity Label-MS Homo sapiens
32 ATP5F1 515
Co-fractionation Homo sapiens
33 MTCH2 23788
Co-fractionation Homo sapiens
34 EGFR 1956
Affinity Capture-MS Homo sapiens
35 PDHB 5162
Co-fractionation Homo sapiens
36 NDUFA4 4697
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
37 COX5B 1329
Affinity Capture-MS Homo sapiens
38 NDUFA8 4702
Co-fractionation Homo sapiens
39 OCIAD1 54940
Co-fractionation Homo sapiens
40 TMEM70  
Proximity Label-MS Homo sapiens
41 MINOS1  
Co-fractionation Homo sapiens
42 Smc3 13006
Affinity Capture-MS Mus musculus
43 LRPPRC 10128
Affinity Capture-Western Homo sapiens
44 SLC9A1 6548
Affinity Capture-MS Homo sapiens
45 CMC1  
Affinity Capture-MS Homo sapiens
46 MGST1 4257
Co-fractionation Homo sapiens
47 NDUFS8 4728
Co-fractionation Homo sapiens
48 MOV10 4343
Affinity Capture-RNA Homo sapiens
49 CYB5A 1528
Co-fractionation Homo sapiens
50 OGDH 4967
Co-fractionation Homo sapiens
51 TOMM5  
Co-fractionation Homo sapiens
52 MGST3 4259
Co-fractionation Homo sapiens
53 COQ9  
Affinity Capture-MS Homo sapiens
54 ATP6V1A 523
Co-fractionation Homo sapiens
55 NDUFB8 4714
Co-fractionation Homo sapiens
56 COX1 4512
Affinity Capture-Western Homo sapiens
57 COX2 4513
Affinity Capture-Western Homo sapiens
58 C20orf24  
Co-fractionation Homo sapiens
59 NDUFS3 4722
Co-fractionation Homo sapiens
60 CYC1 1537
Co-fractionation Homo sapiens
61 UQCRC1 7384
Co-fractionation Homo sapiens
62 CUX1 1523
Affinity Capture-MS Homo sapiens
63 CHCHD10  
Affinity Capture-MS Homo sapiens
64 COX7C 1350
Co-fractionation Homo sapiens
65 ATP5C1 509
Co-fractionation Homo sapiens
66 UQCRQ 27089
Co-fractionation Homo sapiens
67 RPA3 6119
Proximity Label-MS Homo sapiens
68 SURF1  
Proximity Label-MS Homo sapiens
69 ATP5O 539
Co-fractionation Homo sapiens
70 NIPSNAP1 8508
Co-fractionation Homo sapiens
71 ATP5B 506
Co-fractionation Homo sapiens
72 SCCPDH 51097
Co-fractionation Homo sapiens
73 NDUFS2 4720
Co-fractionation Homo sapiens
74 NDUFS7 374291
Co-fractionation Homo sapiens
75 COX3 4514
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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