Gene description for Smc3
Gene name structural maintenance of chromosomes 3
Gene symbol Smc3
Other names/aliases Bamacan
Cspg6
HCAP
Mmip1
SMC-3
SmcD
Species Mus musculus
 Database cross references - Smc3
ExoCarta ExoCarta_13006
Vesiclepedia VP_13006
Entrez Gene 13006
UniProt Q9CW03  
 Smc3 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Smc3
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    chromatin binding GO:0003682 IDA
    double-stranded DNA binding GO:0003690 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    mediator complex binding GO:0036033 IDA
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    beta-tubulin binding GO:0048487 IEA
    beta-tubulin binding GO:0048487 ISO
    dynein complex binding GO:0070840 ISO
    dynein complex binding GO:0070840 ISS
Biological Process
    regulation of DNA replication GO:0006275 ISO
    regulation of DNA replication GO:0006275 ISS
    DNA repair GO:0006281 IEA
    sister chromatid cohesion GO:0007062 IEA
    sister chromatid cohesion GO:0007062 ISO
    mitotic sister chromatid cohesion GO:0007064 IBA
    stem cell population maintenance GO:0019827 IMP
    cell division GO:0051301 IEA
    meiotic cell cycle GO:0051321 IPI
    meiotic cell cycle GO:0051321 ISO
    meiotic cell cycle GO:0051321 ISS
    mitotic spindle assembly GO:0090307 IEA
    mitotic spindle assembly GO:0090307 ISO
Subcellular Localization
    chromosome, centromeric region GO:0000775 IEA
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    synaptonemal complex GO:0000795 ISO
    lateral element GO:0000800 IDA
    basement membrane GO:0005604 ISO
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cohesin complex GO:0008278 IDA
    cohesin complex GO:0008278 ISO
    nuclear matrix GO:0016363 ISO
    nuclear matrix GO:0016363 ISS
    mitotic cohesin complex GO:0030892 IBA
    mitotic cohesin complex GO:0030892 IEA
    mitotic cohesin complex GO:0030892 ISO
    meiotic cohesin complex GO:0030893 IDA
    meiotic cohesin complex GO:0030893 ISO
    meiotic cohesin complex GO:0030893 ISS
    mitotic spindle pole GO:0097431 ISO
    mitotic spindle pole GO:0097431 ISS
 Experiment description of studies that identified Smc3 in exosomes
1
Experiment ID 263
MISEV standards
Biophysical techniques
CD63|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smc3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 Elf5  
Affinity Capture-MS Mus musculus
3 Srpr  
Co-fractionation Mus musculus
4 TRIM38 10475
Affinity Capture-MS Homo sapiens
5 COA3 28958
Affinity Capture-MS Homo sapiens
6 NCBP1 4686
Affinity Capture-MS Homo sapiens
7 SMC1A 8243
Affinity Capture-MS Homo sapiens
8 Stag3  
Affinity Capture-Western Mus musculus
9 Smc1a  
Co-crystal Structure Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
10 PDS5B 23047
Affinity Capture-MS Homo sapiens
11 Pds5a  
Affinity Capture-MS Mus musculus
12 USP13  
Affinity Capture-MS Homo sapiens
13 ECT2 1894
Affinity Capture-MS Homo sapiens
14 Sycp2  
Affinity Capture-Western Mus musculus
15 Pds5b  
Co-fractionation Mus musculus
16 Rec8  
Affinity Capture-Western Mus musculus
17 TCP11L1 55346
Affinity Capture-MS Homo sapiens
18 Atrx  
Affinity Capture-Western Mus musculus
19 Hormad1  
Affinity Capture-Western Mus musculus
20 RUSC2 9853
Affinity Capture-MS Homo sapiens
21 STAG2 10735
Affinity Capture-MS Homo sapiens
22 Nsmce1  
Co-fractionation Mus musculus
23 Mecp2  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
24 TXN 7295
Affinity Capture-MS Homo sapiens
25 WAPAL  
Affinity Capture-MS Homo sapiens
26 TAF5  
Affinity Capture-MS Homo sapiens
27 Nde1  
Affinity Capture-Western Mus musculus
28 STAG1  
Affinity Capture-MS Homo sapiens
29 Wapal  
Co-fractionation Mus musculus
30 PDS5A 23244
Affinity Capture-MS Homo sapiens
31 KIF22  
Affinity Capture-MS Homo sapiens
32 TAF12  
Affinity Capture-MS Homo sapiens
33 Nanog  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
34 C14orf80  
Affinity Capture-MS Homo sapiens
35 OBSCN 84033
Affinity Capture-MS Homo sapiens
36 IQGAP1 8826
Affinity Capture-MS Homo sapiens
37 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
38 EIF5A 1984
Affinity Capture-MS Homo sapiens
39 KRT6C 286887
Affinity Capture-MS Homo sapiens
40 Smc5  
Co-fractionation Mus musculus
41 TAF1  
Affinity Capture-MS Homo sapiens
42 FYTTD1  
Affinity Capture-MS Homo sapiens
43 Eed  
Affinity Capture-MS Mus musculus
44 Smc1b  
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
45 SMC2 10592
Affinity Capture-MS Homo sapiens
46 ARID5B 84159
Affinity Capture-MS Homo sapiens
47 ZFYVE26 23503
Affinity Capture-MS Homo sapiens
48 Atg16l1  
Affinity Capture-MS Mus musculus
49 GPKOW  
Affinity Capture-MS Homo sapiens
50 RAD21 5885
Affinity Capture-MS Homo sapiens
51 Fancd2  
Affinity Capture-MS Mus musculus
52 MVD 4597
Affinity Capture-MS Homo sapiens
53 PDF  
Affinity Capture-MS Homo sapiens
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