Gene description for OBSCN
Gene name obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Gene symbol OBSCN
Other names/aliases ARHGEF30
Species Homo sapiens
 Database cross references - OBSCN
ExoCarta ExoCarta_84033
Entrez Gene 84033
HGNC 15719
MIM 608616
UniProt Q5VST9  
 OBSCN identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for OBSCN
Molecular Function
    ankyrin binding GO:0030506 IPI
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    calmodulin binding GO:0005516 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    titin binding GO:0031432 IPI
    structural constituent of muscle GO:0008307 NAS
    metal ion binding GO:0046872 IEA
Biological Process
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    multicellular organismal development GO:0007275 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    apoptotic signaling pathway GO:0097190 TAS
    protein phosphorylation GO:0006468 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    sarcomere organization GO:0045214 TAS
    protein localization to M-band GO:0036309 ISS
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    M band GO:0031430 ISS
    Z disc GO:0030018 ISS
    cytosol GO:0005829 TAS
    myofibril GO:0030016 NAS
 Experiment description of studies that identified OBSCN in exosomes
Experiment ID 224
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for OBSCN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TTN 7273
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 ANK1 286
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which OBSCN is involved
G alpha (12/13) signalling events TAS Reactome
NRAGE signals death through JNK TAS Reactome
Rho GTPase cycle TAS Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here