Gene description for ALG2
Gene name ALG2, alpha-1,3/1,6-mannosyltransferase
Gene symbol ALG2
Other names/aliases CDGIi
CMS14
CMSTA3
NET38
hALPG2
Species Homo sapiens
 Database cross references - ALG2
ExoCarta ExoCarta_85365
Vesiclepedia VP_85365
Entrez Gene 85365
HGNC 23159
MIM 607905
UniProt Q9H553  
 ALG2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ALG2
Molecular Function
    alpha-1,3-mannosyltransferase activity GO:0000033 IBA
    alpha-1,3-mannosyltransferase activity GO:0000033 IDA
    alpha-1,3-mannosyltransferase activity GO:0000033 TAS
    GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity GO:0004378 IDA
    protein binding GO:0005515 IPI
    GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase activity GO:0102704 IDA
Biological Process
    protein glycosylation GO:0006486 IGI
    protein N-linked glycosylation GO:0006487 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IBA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IGI
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 IDA
 Experiment description of studies that identified ALG2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ALG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 FAM20C 56975
Affinity Capture-MS Homo sapiens
5 VPS28 51160
Affinity Capture-Western Homo sapiens
6 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
7 ATP1A3 478
Affinity Capture-MS Homo sapiens
8 CD79B 974
Affinity Capture-MS Homo sapiens
9 PDCD6IP 10015
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 HMOX2 3163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMP1 3916
Co-fractionation Homo sapiens
12 ANXA7 310
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 LDHA 3939
Affinity Capture-MS Homo sapiens
14 TSG101 7251
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 RPL21 6144
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
17 PARK2  
Affinity Capture-MS Homo sapiens
18 HSP90AA1 3320
Co-fractionation Homo sapiens
19 PLSCR3 57048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 POTEF 728378
Affinity Capture-MS Homo sapiens
21 VSIG4  
Affinity Capture-MS Homo sapiens
22 ANXA11 311
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 VPS37A 137492
Affinity Capture-Western Homo sapiens
24 SEC31A 22872
Affinity Capture-Western Homo sapiens
25 PTK2B 2185
Affinity Capture-Western Homo sapiens
26 OBSCN 84033
Cross-Linking-MS (XL-MS) Homo sapiens
27 SHISA5 51246
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 PTPN23 25930
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 KRAS 3845
Negative Genetic Homo sapiens
30 TECR 9524
Affinity Capture-MS Homo sapiens
31 SLC22A4 6583
Affinity Capture-MS Homo sapiens
32 ALG2 85365
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 TACSTD2 4070
Affinity Capture-MS Homo sapiens
34 SRI 6717
Reconstituted Complex Homo sapiens
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