Gene description for PTK2B
Gene name protein tyrosine kinase 2 beta
Gene symbol PTK2B
Other names/aliases CADTK
CAKB
FADK2
FAK2
PKB
PTK
PYK2
RAFTK
Species Homo sapiens
 Database cross references - PTK2B
ExoCarta ExoCarta_2185
Entrez Gene 2185
HGNC 9612
MIM 601212
UniProt Q14289  
 PTK2B identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for PTK2B
Molecular Function
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    calmodulin-dependent protein kinase activity GO:0004683 ISS
    protein complex binding GO:0032403 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    signal transducer activity GO:0004871 NAS
    receptor binding GO:0005102 IBA
    3-phosphoinositide-dependent protein kinase binding GO:0043423 IEA
    N-methyl-D-aspartate selective glutamate receptor activity GO:0004972 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of synaptic transmission, glutamatergic GO:0051968 ISS
    cellular defense response GO:0006968 ISS
    regulation of inositol trisphosphate biosynthetic process GO:0032960 ISS
    positive regulation of nitric-oxide synthase activity GO:0051000 IEA
    positive regulation of actin filament polymerization GO:0030838 IMP
    response to hypoxia GO:0001666 IEA
    positive regulation of translation GO:0045727 IEA
    regulation of macrophage chemotaxis GO:0010758 ISS
    positive regulation of JNK cascade GO:0046330 IMP
    positive regulation of cell-matrix adhesion GO:0001954 IMP
    negative regulation of muscle cell apoptotic process GO:0010656 IEA
    positive regulation of JUN kinase activity GO:0043507 IEA
    positive regulation of protein kinase activity GO:0045860 IMP
    peptidyl-tyrosine autophosphorylation GO:0038083 ISS
    positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000060 IEA
    response to lithium ion GO:0010226 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    response to immobilization stress GO:0035902 IEA
    response to drug GO:0042493 IEA
    negative regulation of bone mineralization GO:0030502 ISS
    innate immune response GO:0045087 IBA
    positive regulation of cell proliferation GO:0008284 IMP
    regulation of calcium-mediated signaling GO:0050848 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    marginal zone B cell differentiation GO:0002315 ISS
    activation of Janus kinase activity GO:0042976 IMP
    neuron projection development GO:0031175 IEA
    positive regulation of B cell chemotaxis GO:2000538 ISS
    regulation of actin cytoskeleton reorganization GO:2000249 ISS
    blood vessel endothelial cell migration GO:0043534 IEA
    positive regulation of endothelial cell migration GO:0010595 IDA
    positive regulation of cell growth GO:0030307 IEA
    ionotropic glutamate receptor signaling pathway GO:0035235 ISS
    response to calcium ion GO:0051592 IEA
    response to cocaine GO:0042220 IEA
    angiogenesis GO:0001525 IBA
    protein phosphorylation GO:0006468 TAS
    cell migration GO:0016477 IBA
    signal transduction GO:0007165 TAS
    signal complex assembly GO:0007172 IEA
    protein autophosphorylation GO:0046777 TAS
    regulation of N-methyl-D-aspartate selective glutamate receptor activity GO:2000310 ISS
    sprouting angiogenesis GO:0002040 ISS
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 ISS
    negative regulation of potassium ion transport GO:0043267 IDA
    response to hormone GO:0009725 IEA
    cytoskeleton organization GO:0007010 IBA
    regulation of nitric oxide biosynthetic process GO:0045428 IEA
    apoptotic process GO:0006915 TAS
    response to osmotic stress GO:0006970 IEA
    chemokine-mediated signaling pathway GO:0070098 ISS
    regulation of cell adhesion GO:0030155 IMP
    negative regulation of cell proliferation GO:0008285 IMP
    regulation of cGMP biosynthetic process GO:0030826 IEA
    long-term synaptic potentiation GO:0060291 ISS
    response to glucose GO:0009749 IEA
    response to cAMP GO:0051591 IEA
    tumor necrosis factor-mediated signaling pathway GO:0033209 IMP
    response to ethanol GO:0045471 IEA
    focal adhesion assembly GO:0048041 IEA
    response to mechanical stimulus GO:0009612 IEA
    oocyte maturation GO:0001556 IEA
    cell differentiation GO:0030154 IBA
    bone resorption GO:0045453 ISS
    protein complex assembly GO:0006461 TAS
    response to hydrogen peroxide GO:0042542 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    integrin-mediated signaling pathway GO:0007229 IMP
    response to stress GO:0006950 TAS
    positive regulation of reactive oxygen species metabolic process GO:2000379 IEA
    regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000058 IDA
    cell adhesion GO:0007155 IBA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 ISS
    positive regulation of neuron projection development GO:0010976 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    cell surface receptor signaling pathway GO:0007166 IMP
    MAPK cascade GO:0000165 IEA
    negative regulation of myeloid cell differentiation GO:0045638 IMP
    positive regulation of excitatory postsynaptic membrane potential GO:2000463 ISS
    regulation of cGMP-mediated signaling GO:0010752 IEA
    cellular response to retinoic acid GO:0071300 IMP
    regulation of establishment of cell polarity GO:2000114 ISS
    positive regulation of cell migration GO:0030335 IMP
    regulation of cell shape GO:0008360 IMP
    positive regulation of angiogenesis GO:0045766 IEA
    stress fiber assembly GO:0043149 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    negative regulation of neuron apoptotic process GO:0043524 ISS
    glial cell proliferation GO:0014009 IEA
Subcellular Localization
    lamellipodium GO:0030027 IDA
    nucleoplasm GO:0005654 IDA
    neuronal cell body GO:0043025 ISS
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IEA
    dendrite GO:0030425 ISS
    nucleus GO:0005634 IDA
    axon GO:0030424 IEA
    cell cortex GO:0005938 IEA
    cytoskeleton GO:0005856 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    N-methyl-D-aspartate selective glutamate receptor complex GO:0017146 ISS
    cytoplasm GO:0005737 IDA
    growth cone GO:0030426 ISS
    cell body GO:0044297 ISS
    apical dendrite GO:0097440 ISS
    focal adhesion GO:0005925 IDA
    postsynaptic density GO:0014069 ISS
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
 Experiment description of studies that identified PTK2B in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTK2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSN 2934
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
2 RASA1 5921
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
3 NEDD9 4739
Affinity Capture-Western Homo sapiens
4 TGFB1I1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 LCK 3932
Affinity Capture-Western Homo sapiens
6 PRKCD 5580
Affinity Capture-Western Homo sapiens
7 ERBB2 2064
Invivo Homo sapiens
8 KCNA2  
Affinity Capture-Western Homo sapiens
9 GRB2 2885
Invivo Homo sapiens
Invivo Homo sapiens
10 SHC1 6464
Invivo Homo sapiens
Invitro Homo sapiens
11 PITPNM3 83394
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 PXN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 SORBS1 10580
Affinity Capture-Western Homo sapiens
14 PTPN6 5777
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
15 PITPNM1 9600
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 DLG3 1741
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 CRK 1398
Invivo Homo sapiens
Invitro Homo sapiens
18 ITGB3 3690
Invivo Homo sapiens
Invitro Homo sapiens
19 BCAR1 9564
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 CD2AP 23607
Affinity Capture-Western Homo sapiens
21 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
22 SLC2A1 6513
Affinity Capture-Western Homo sapiens
23 TLN1 7094
Affinity Capture-Western Homo sapiens
24 EFS  
Invitro Homo sapiens
25 LPXN  
Affinity Capture-Western Homo sapiens
26 EGFR 1956
Affinity Capture-Western Homo sapiens
27 NPHP1  
Invivo Homo sapiens
Invitro Homo sapiens
28 RB1CC1 9821
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
29 DLG4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 LYN 4067
Affinity Capture-Western Homo sapiens
31 PTPN11 5781
Invivo Homo sapiens
Invitro Homo sapiens
32 CBL 867
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
33 JAK3 3718
Affinity Capture-Western Homo sapiens
34 PTPN12  
Invivo Homo sapiens
Invitro Homo sapiens
35 IL7R  
Affinity Capture-Western Homo sapiens
36 GIT1 28964
Affinity Capture-Western Homo sapiens
37 FYN 2534
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
38 JAK1 3716
Affinity Capture-Western Homo sapiens
39 PITPNM2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
40 CCR5 1234
Invivo Homo sapiens
41 PTK2B 2185
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
42 ZAP70 7535
Invivo Homo sapiens
43 DCC  
Affinity Capture-Western Homo sapiens
44 GNA13 10672
Affinity Capture-Western Homo sapiens
45 SRC 6714
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
46 SYK 6850
Affinity Capture-Western Homo sapiens
47 GRIN2A  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
48 EWSR1 2130
Invivo Homo sapiens
Invitro Homo sapiens
49 ITGB2 3689
Affinity Capture-Western Homo sapiens
50 JAK2 3717
Affinity Capture-Western Homo sapiens
51 ARGBP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 ASAP2 8853
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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