Gene description for BCAR1
Gene name breast cancer anti-estrogen resistance 1
Gene symbol BCAR1
Other names/aliases CAS
CAS1
CASS1
CRKAS
P130Cas
Species Homo sapiens
 Database cross references - BCAR1
ExoCarta ExoCarta_9564
Vesiclepedia VP_9564
Entrez Gene 9564
HGNC 971
MIM 602941
UniProt P56945  
 BCAR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for BCAR1
Molecular Function
    signal transducer activity GO:0004871 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    regulation of cell growth GO:0001558 TAS
    insulin receptor signaling pathway GO:0008286 ISS
    cell migration GO:0016477 IDA
    positive regulation of endothelial cell migration GO:0010595 IDA
    blood coagulation GO:0007596 TAS
    hepatocyte growth factor receptor signaling pathway GO:0048012 IMP
    cell proliferation GO:0008283 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    platelet-derived growth factor receptor signaling pathway GO:0048008 ISS
    platelet activation GO:0030168 TAS
    antigen receptor-mediated signaling pathway GO:0050851 IDA
    cell division GO:0051301 NAS
    cell adhesion GO:0007155 IEA
    T cell receptor signaling pathway GO:0050852 NAS
    cellular response to hepatocyte growth factor stimulus GO:0035729 IMP
    positive regulation of cell migration GO:0030335 IDA
    G-protein coupled receptor signaling pathway GO:0007186 NAS
    regulation of apoptotic process GO:0042981 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 ISS
    cell chemotaxis GO:0060326 IMP
    B cell receptor signaling pathway GO:0050853 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 ISS
    integrin-mediated signaling pathway GO:0007229 IDA
    actin filament organization GO:0007015 IDA
Subcellular Localization
    actin cytoskeleton GO:0015629 IEA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    ruffle GO:0001726 IDA
    focal adhesion GO:0005925 ISS
    lamellipodium GO:0030027 IEA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified BCAR1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BCAR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 DOCK1 1793
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 HSPA5 3309
Two-hybrid Homo sapiens
4 NCK1 4690
Invivo Homo sapiens
5 BCAR3 8412
Affinity Capture-Western Homo sapiens
6 GRB2 2885
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 PXN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 RICS  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 INPPL1 3636
Invivo Homo sapiens
Invitro Homo sapiens
10 SH2D3A  
Affinity Capture-Western Homo sapiens
11 NEDD9 4739
Invivo Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 SH2D3C 10044
Affinity Capture-Western Homo sapiens
13 PTPRH 5794
Invivo Homo sapiens
Invitro Homo sapiens
14 TNS1 7145
Affinity Capture-Western Homo sapiens
15 LCK 3932
Reconstituted Complex Homo sapiens
16 YWHAZ 7534
Two-hybrid Homo sapiens
17 ABL1 25
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
18 NPHP1  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
19 VCL 7414
Affinity Capture-Western Homo sapiens
20 FXYD6  
Two-hybrid Homo sapiens
21 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 PTPN1 5770
Invivo Homo sapiens
Invitro Homo sapiens
23 LYN 4067
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
24 PTPN11 5781
Affinity Capture-Western Homo sapiens
25 Tuba1a 22142
Two-hybrid Mus musculus
26 PTPN12  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
27 E2F2 1870
Two-hybrid Homo sapiens
28 TRIP6  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 HCK 3055
Reconstituted Complex Homo sapiens
30 FYN 2534
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 CRKL 1399
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 CRK 1398
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 PTK2B 2185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 FES 2242
Invivo Homo sapiens
35 VPS11 55823
Two-hybrid Homo sapiens
36 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
37 SNIP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 EFS  
Two-hybrid Homo sapiens
40 RAPGEF1 2889
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
41 MAPK8 5599
Affinity Capture-Western Homo sapiens
42 PIK3R1 5295
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
43 PPP1R15A  
Two-hybrid Homo sapiens
44 CD2AP 23607
Invitro Homo sapiens
45 ID2  
Affinity Capture-Western Homo sapiens
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