Gene description for TSEN34
Gene name TSEN34 tRNA splicing endonuclease subunit
Gene symbol TSEN34
Other names/aliases LENG5
PCH2C
SEN34
SEN34L
Species Homo sapiens
 Database cross references - TSEN34
ExoCarta ExoCarta_79042
Vesiclepedia VP_79042
Entrez Gene 79042
HGNC 15506
MIM 608754
UniProt Q9BSV6  
 TSEN34 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TSEN34
Molecular Function
    tRNA-intron endonuclease activity GO:0000213 IBA
    nucleic acid binding GO:0003676 IEA
    lyase activity GO:0016829 IEA
Biological Process
    tRNA-type intron splice site recognition and cleavage GO:0000379 IBA
    mRNA processing GO:0006397 IEA
Subcellular Localization
    tRNA-intron endonuclease complex GO:0000214 IEA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IEA
 Experiment description of studies that identified TSEN34 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TSEN34
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPD 3184
Two-hybrid Homo sapiens
2 SNX27 81609
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 FBXW11  
Affinity Capture-MS Homo sapiens
5 ASF1A 25842
Proximity Label-MS Homo sapiens
6 RAB3GAP2 25782
Co-fractionation Homo sapiens
7 TP53BP1 7158
Negative Genetic Homo sapiens
8 TSEN15 116461
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CLP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BCAR1 9564
Co-fractionation Homo sapiens
11 TSEN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SSB 6741
Affinity Capture-MS Homo sapiens
13 DDX58 23586
Affinity Capture-RNA Homo sapiens
14 Batf3  
Affinity Capture-MS Mus musculus
15 SOX15  
Co-fractionation Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 EYA3  
Affinity Capture-MS Homo sapiens
18 TSEN54  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 METTL3  
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 RAB3GAP1 22930
Co-fractionation Homo sapiens
22 C9orf72  
Affinity Capture-MS Homo sapiens
23 AR 367
Affinity Capture-MS Homo sapiens
24 ARMCX3 51566
Co-fractionation Homo sapiens
25 GNE 10020
Co-fractionation Homo sapiens
26 L1TD1  
Affinity Capture-MS Homo sapiens
27 GABPA 2551
Co-fractionation Homo sapiens
28 Clp1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which TSEN34 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome





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