Gene description for GNE
Gene name glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene symbol GNE
Other names/aliases DMRV
GLCNE
IBM2
NM
Uae1
Species Homo sapiens
 Database cross references - GNE
ExoCarta ExoCarta_10020
Vesiclepedia VP_10020
Entrez Gene 10020
HGNC 23657
MIM 603824
UniProt Q9Y223  
 GNE identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for GNE
Molecular Function
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IBA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IDA
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 IMP
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 TAS
    N-acylmannosamine kinase activity GO:0009384 IBA
    N-acylmannosamine kinase activity GO:0009384 IMP
    N-acylmannosamine kinase activity GO:0009384 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    N-acetylglucosamine biosynthetic process GO:0006045 IEA
    UDP-N-acetylglucosamine metabolic process GO:0006047 IEA
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 IMP
    N-acetylneuraminate biosynthetic process GO:0046380 IMP
    carbohydrate phosphorylation GO:0046835 IEA
    glycosylation GO:0070085 IMP
Subcellular Localization
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GNE in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 ZRANB1 54764
Affinity Capture-MS Homo sapiens
3 KRT83  
Two-hybrid Homo sapiens
4 KRTAP9-3  
Two-hybrid Homo sapiens
5 KRTAP3-3  
Two-hybrid Homo sapiens
6 KRT85  
Two-hybrid Homo sapiens
7 KRTAP19-7  
Two-hybrid Homo sapiens
8 MGAT5B  
Two-hybrid Homo sapiens
9 TIMM50 92609
Affinity Capture-MS Homo sapiens
10 TSEN34 79042
Co-fractionation Homo sapiens
11 CYSRT1 375791
Two-hybrid Homo sapiens
12 KRTAP11-1  
Two-hybrid Homo sapiens
13 SPRY2 10253
Two-hybrid Homo sapiens
14 CUL3 8452
Affinity Capture-MS Homo sapiens
15 TSPAN4 7106
Two-hybrid Homo sapiens
16 SMARCAD1  
Co-fractionation Homo sapiens
17 C2orf68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 KRTAP4-4  
Two-hybrid Homo sapiens
19 KRTAP10-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 KRTAP4-1  
Two-hybrid Homo sapiens
21 GTPBP3  
Two-hybrid Homo sapiens
22 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
23 ADAMTSL4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 KCTD12 115207
Co-fractionation Homo sapiens
26 KRT34  
Two-hybrid Homo sapiens
27 NBPF19  
Two-hybrid Homo sapiens
28 SPRY3  
Two-hybrid Homo sapiens
29 MDFI  
Two-hybrid Homo sapiens
30 KRT86  
Two-hybrid Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 KRTAP5-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 EGFL7 51162
Two-hybrid Homo sapiens
34 ZNF764 92595
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KRTAP1-1  
Two-hybrid Homo sapiens
36 KRTAP10-3  
Two-hybrid Homo sapiens
37 HOXA1 3198
Two-hybrid Homo sapiens
38 GNE 10020
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 VWC2  
Two-hybrid Homo sapiens
40 TRIM42  
Two-hybrid Homo sapiens
41 PRICKLE4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
43 KRTAP9-8  
Two-hybrid Homo sapiens
44 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CFP  
Two-hybrid Homo sapiens
46 HOXC5  
Affinity Capture-MS Homo sapiens
47 KRTAP4-12  
Two-hybrid Homo sapiens
48 ZNF213  
Affinity Capture-MS Homo sapiens
49 KRTAP17-1  
Two-hybrid Homo sapiens
50 KRTAP6-2  
Two-hybrid Homo sapiens
51 KRTAP19-2  
Two-hybrid Homo sapiens
52 SSC4D  
Two-hybrid Homo sapiens
53 RPA3 6119
Proximity Label-MS Homo sapiens
54 VCP 7415
Affinity Capture-MS Homo sapiens
55 WWOX 51741
Two-hybrid Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 KRTAP10-9  
Two-hybrid Homo sapiens
58 SCGN  
Affinity Capture-MS Homo sapiens
59 SPRY1 10252
Two-hybrid Homo sapiens
60 CENPM  
Affinity Capture-MS Homo sapiens
61 TRIM27  
Two-hybrid Homo sapiens
62 WDR83  
Two-hybrid Homo sapiens
63 KRTAP3-1  
Two-hybrid Homo sapiens
64 FBXW7  
Affinity Capture-MS Homo sapiens
65 KRTAP4-11  
Two-hybrid Homo sapiens
66 KRT40  
Two-hybrid Homo sapiens
67 KRTAP9-2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
68 RAB3GAP1 22930
Co-fractionation Homo sapiens
69 GAS2L2  
Affinity Capture-MS Homo sapiens
70 ECM1 1893
Two-hybrid Homo sapiens
71 KRTAP13-3  
Two-hybrid Homo sapiens
72 RBM12 10137
Co-fractionation Homo sapiens
73 KRTAP13-2  
Two-hybrid Homo sapiens
74 KRTAP12-3  
Two-hybrid Homo sapiens
75 GABPA 2551
Co-fractionation Homo sapiens
76 KRTAP1-3  
Two-hybrid Homo sapiens
77 KRT31 3881
Two-hybrid Homo sapiens
78 KRTAP6-1  
Two-hybrid Homo sapiens
79 KRTAP6-3  
Two-hybrid Homo sapiens
80 KRTAP10-5  
Two-hybrid Homo sapiens
81 EP300 2033
Affinity Capture-MS Homo sapiens
82 KPRP 448834
Two-hybrid Homo sapiens
83 C22orf39  
Two-hybrid Homo sapiens
84 RAB3GAP2 25782
Co-fractionation Homo sapiens
View the network image/svg+xml



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