Gene description for GNE
Gene name glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene symbol GNE
Other names/aliases DMRV
GLCNE
IBM2
NM
Uae1
Species Homo sapiens
 Database cross references - GNE
ExoCarta ExoCarta_10020
Entrez Gene 10020
HGNC 23657
MIM 603824
UniProt Q9Y223  
 GNE identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for GNE
Molecular Function
    UDP-N-acetylglucosamine 2-epimerase activity GO:0008761 TAS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    hydrolase activity, hydrolyzing O-glycosyl compounds GO:0004553 IEA
    N-acylmannosamine kinase activity GO:0009384 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    UDP-N-acetylglucosamine metabolic process GO:0006047 IEA
    N-acetylneuraminate metabolic process GO:0006054 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    carbohydrate phosphorylation GO:0046835 TAS
    cell adhesion GO:0007155 TAS
    N-acetylglucosamine biosynthetic process GO:0006045 IEA
    post-translational protein modification GO:0043687 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GNE in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNE
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GNE is involved
PathwayEvidenceSource
Sialic acid metabolism TAS Reactome





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