Gene description for RAB3GAP1
Gene name RAB3 GTPase activating protein subunit 1 (catalytic)
Gene symbol RAB3GAP1
Other names/aliases P130
RAB3GAP
RAB3GAP130
WARBM1
Species Homo sapiens
 Database cross references - RAB3GAP1
ExoCarta ExoCarta_22930
Vesiclepedia VP_22930
Entrez Gene 22930
HGNC 17063
MIM 602536
UniProt Q15042  
 RAB3GAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for RAB3GAP1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 IMP
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
Biological Process
    brain development GO:0007420 IBA
    brain development GO:0007420 IMP
    hypothalamus development GO:0021854 IMP
    lipid droplet organization GO:0034389 IMP
    camera-type eye development GO:0043010 IMP
    regulation of GTPase activity GO:0043087 IDA
    positive regulation of GTPase activity GO:0043547 IMP
    positive regulation of GTPase activity GO:0043547 ISS
    regulation of short-term neuronal synaptic plasticity GO:0048172 ISS
    excitatory postsynaptic potential GO:0060079 ISS
    face morphogenesis GO:0060325 IMP
    positive regulation of glutamate neurotransmitter secretion in response to membrane depolarization GO:0061646 ISS
    establishment of protein localization to endoplasmic reticulum membrane GO:0097051 IMP
    positive regulation of protein lipidation GO:1903061 IMP
    regulation of calcium ion-dependent exocytosis of neurotransmitter GO:1903233 ISS
    positive regulation of endoplasmic reticulum tubular network organization GO:1903373 IMP
    positive regulation of autophagosome assembly GO:2000786 IBA
    positive regulation of autophagosome assembly GO:2000786 IMP
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    lipid droplet GO:0005811 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
    extracellular exosome GO:0070062 HDA
    endoplasmic reticulum tubular network GO:0071782 IDA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified RAB3GAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RAB3GAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 B3GAT1  
Proximity Label-MS Homo sapiens
5 PER1  
Affinity Capture-MS Homo sapiens
6 PAXIP1  
Affinity Capture-MS Homo sapiens
7 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 METTL7A 25840
Proximity Label-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 GJD3  
Proximity Label-MS Homo sapiens
11 PKD1L2  
Affinity Capture-MS Homo sapiens
12 DNAJC25 548645
Proximity Label-MS Homo sapiens
13 PTPN1 5770
Proximity Label-MS Homo sapiens
14 STX4 6810
Proximity Label-MS Homo sapiens
15 GNE 10020
Co-fractionation Homo sapiens
16 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
17 SNRNP70 6625
Co-fractionation Homo sapiens
18 BTN2A1 11120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 COPB2 9276
Affinity Capture-MS Homo sapiens
20 GUSBP5  
Affinity Capture-MS Homo sapiens
21 SSR1 6745
Proximity Label-MS Homo sapiens
22 SRP14 6727
Affinity Capture-MS Homo sapiens
23 RPN2 6185
Proximity Label-MS Homo sapiens
24 TULP3 7289
Affinity Capture-MS Homo sapiens
25 SLC25A46 91137
Proximity Label-MS Homo sapiens
26 PRTN3 5657
Affinity Capture-MS Homo sapiens
27 WDR7 23335
Affinity Capture-Western Homo sapiens
28 EBAG9 9166
Proximity Label-MS Homo sapiens
29 MARCKS 4082
Proximity Label-MS Homo sapiens
30 LMAN1 3998
Affinity Capture-MS Homo sapiens
31 KIAA1715 80856
Proximity Label-MS Homo sapiens
32 PXMP2  
Proximity Label-MS Homo sapiens
33 RAB5A 5868
Proximity Label-MS Homo sapiens
34 EMD 2010
Proximity Label-MS Homo sapiens
35 TGM2 7052
Affinity Capture-MS Homo sapiens
36 COPE 11316
Affinity Capture-MS Homo sapiens
37 GALNT12 79695
Affinity Capture-MS Homo sapiens
38 RCCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 VCP 7415
Affinity Capture-MS Homo sapiens
40 TRIM21 6737
Affinity Capture-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 NCOA7  
Co-fractionation Homo sapiens
43 RPN1 6184
Proximity Label-MS Homo sapiens
44 BRAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ELOVL5 60481
Proximity Label-MS Homo sapiens
46 SEC61B 10952
Proximity Label-MS Homo sapiens
47 REEP5 7905
Proximity Label-MS Homo sapiens
48 SEC63 11231
Proximity Label-MS Homo sapiens
49 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 LRRC59 55379
Proximity Label-MS Homo sapiens
51 EGLN3  
Affinity Capture-MS Homo sapiens
52 REEP3 221035
Affinity Capture-MS Homo sapiens
53 BCAP31 10134
Proximity Label-MS Homo sapiens
54 HSD3B7 80270
Proximity Label-MS Homo sapiens
55 NR3C1 2908
Proximity Label-MS Homo sapiens
56 STIM1 6786
Proximity Label-MS Homo sapiens
57 FKBP8 23770
Proximity Label-MS Homo sapiens
58 RAB39B 116442
Two-hybrid Homo sapiens
59 YIPF5 81555
Affinity Capture-MS Homo sapiens
60 DUSP16  
Affinity Capture-MS Homo sapiens
61 OCIAD1 54940
Proximity Label-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 ATP2A1 487
Proximity Label-MS Homo sapiens
64 RAB9A 9367
Proximity Label-MS Homo sapiens
65 GOLGA1  
Proximity Label-MS Homo sapiens
66 SIRT2 22933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 COPA 1314
Affinity Capture-MS Homo sapiens
68 DCAF15  
Affinity Capture-MS Homo sapiens
69 RAB22A 57403
Two-hybrid Homo sapiens
70 DMXL2  
Affinity Capture-Western Homo sapiens
71 RAB39A 54734
Two-hybrid Homo sapiens
72 FEZ1  
Two-hybrid Homo sapiens
73 RPH3AL 9501
Reconstituted Complex Homo sapiens
74 FASN 2194
Negative Genetic Homo sapiens
75 TSEN34 79042
Co-fractionation Homo sapiens
76 GMFB 2764
Affinity Capture-MS Homo sapiens
77 RAB3GAP2 25782
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
78 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 COG7 91949
Affinity Capture-MS Homo sapiens
80 C6orf141  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ERGIC2 51290
Proximity Label-MS Homo sapiens
82 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
83 FEZ2 9637
Two-hybrid Homo sapiens
84 SEC62 7095
Proximity Label-MS Homo sapiens
85 FIS1 51024
Proximity Label-MS Homo sapiens
86 OC90  
Affinity Capture-MS Homo sapiens
87 DNAJC1 64215
Proximity Label-MS Homo sapiens
88 RAB5C 5878
Proximity Label-MS Homo sapiens
89 CKAP4 10970
Proximity Label-MS Homo sapiens
90 PSMC4 5704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 GABPA 2551
Co-fractionation Homo sapiens
92 ALOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PCYT1A 5130
Affinity Capture-MS Homo sapiens
94 BCAT1 586
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 DERL1 79139
Proximity Label-MS Homo sapiens
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