Gene description for RAB3GAP1
Gene name RAB3 GTPase activating protein subunit 1 (catalytic)
Gene symbol RAB3GAP1
Other names/aliases P130
RAB3GAP
RAB3GAP130
WARBM1
Species Homo sapiens
 Database cross references - RAB3GAP1
ExoCarta ExoCarta_22930
Entrez Gene 22930
HGNC 17063
MIM 602536
UniProt Q15042  
 RAB3GAP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 19056867    
 Gene ontology annotations for RAB3GAP1
Molecular Function
    Rab GTPase binding GO:0017137 IPI
    GTPase activator activity GO:0005096 IMP
    Rab guanyl-nucleotide exchange factor activity GO:0017112 IMP
Biological Process
    hypothalamus development GO:0021854 IMP
    regulation of calcium ion-dependent exocytosis of neurotransmitter GO:1903233 ISS
    brain development GO:0007420 IMP
    camera-type eye development GO:0043010 IMP
    lipid particle organization GO:0034389 IMP
    positive regulation of endoplasmic reticulum tubular network organization GO:1903373 IMP
    positive regulation of glutamate neurotransmitter secretion in response to membrane depolarization GO:0061646 ISS
    face morphogenesis GO:0060325 IMP
    positive regulation of GTPase activity GO:0043547 ISS
    establishment of protein localization to endoplasmic reticulum membrane GO:0097051 IMP
    regulation of GTPase activity GO:0043087 IDA
    regulation of excitatory postsynaptic membrane potential GO:0060079 ISS
    regulation of short-term neuronal synaptic plasticity GO:0048172 ISS
Subcellular Localization
    protein complex GO:0043234 IDA
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum tubular network GO:0071782 IDA
 Experiment description of studies that identified RAB3GAP1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RAB3GAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DMXL2  
Affinity Capture-Western Homo sapiens
2 WDR7 23335
Affinity Capture-Western Homo sapiens
3 RPH3AL 9501
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which RAB3GAP1 is involved
No pathways found





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