Gene description for DOCK5
Gene name dedicator of cytokinesis 5
Gene symbol DOCK5
Other names/aliases -
Species Homo sapiens
 Database cross references - DOCK5
ExoCarta ExoCarta_80005
Vesiclepedia VP_80005
Entrez Gene 80005
HGNC 23476
UniProt Q9H7D0  
 DOCK5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for DOCK5
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
Biological Process
    myoblast fusion GO:0007520 IBA
    positive regulation of epithelial cell migration GO:0010634 IMP
    cell migration GO:0016477 IBA
    Rac protein signal transduction GO:0016601 IEA
    bone remodeling GO:0046849 IEA
    podosome assembly GO:0071800 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    negative regulation of vascular associated smooth muscle contraction GO:1904694 IMP
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 IMP
Subcellular Localization
    podosome GO:0002102 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell projection GO:0042995 IEA
    anchoring junction GO:0070161 IEA
 Experiment description of studies that identified DOCK5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOCK5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SGCA  
Affinity Capture-MS Homo sapiens
5 CRK 1398
Affinity Capture-MS Homo sapiens
6 TUBB8 347688
Affinity Capture-MS Homo sapiens
7 SPSB4  
Affinity Capture-MS Homo sapiens
8 KIF20A 10112
Affinity Capture-MS Homo sapiens
9 PLEKHG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PJA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RNASEL 6041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SHMT1 6470
Affinity Capture-MS Homo sapiens
13 FBXW8 26259
Affinity Capture-MS Homo sapiens
14 MYCBP2 23077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 C5orf34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DOCK1 1793
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ATG2B 55102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HSPA8 3312
Affinity Capture-MS Homo sapiens
19 SPSB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 BSG 682
Affinity Capture-MS Homo sapiens
21 BRIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DPYSL4 10570
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
24 Rock1  
Affinity Capture-MS Mus musculus
25 SPSB2  
Affinity Capture-MS Homo sapiens
26 SMEK1 55671
Affinity Capture-MS Homo sapiens
27 SZT2 23334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NOL11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 VSIG4  
Affinity Capture-MS Homo sapiens
30 BCAS3 54828
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ZHX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PIK3CB 5291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CRKL 1399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 GPR182  
Affinity Capture-MS Homo sapiens
35 S100A8 6279
Affinity Capture-MS Homo sapiens
36 GRIN1 2902
Affinity Capture-MS Homo sapiens
37 RAB3GAP1 22930
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 STIL  
Affinity Capture-MS Homo sapiens
39 MICU1 10367
Affinity Capture-MS Homo sapiens
40 SSR4 6748
Affinity Capture-MS Homo sapiens
41 MTR 4548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ATXN1 6310
Two-hybrid Homo sapiens
43 ELMO3 79767
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PRKD2 25865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MADD 8567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ELMO1 9844
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 BAG2 9532
Affinity Capture-MS Homo sapiens
48 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CUL3 8452
Affinity Capture-MS Homo sapiens
50 METTL21B  
Affinity Capture-MS Homo sapiens
51 SDC2 6383
Affinity Capture-MS Homo sapiens
52 WDR59 79726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TUBA4A 7277
Affinity Capture-MS Homo sapiens
54 GPN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 YME1L1 10730
Affinity Capture-MS Homo sapiens
56 USP19 10869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LRCH2  
Affinity Capture-MS Homo sapiens
58 RAC2 5880
Affinity Capture-MS Homo sapiens
59 Lgals3bp 19039
Affinity Capture-MS Mus musculus
60 ATP1A2 477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KIAA0100  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 KIAA0232 9778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 KIF1A 547
Affinity Capture-MS Homo sapiens
64 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 AHI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MCAT 27349
Affinity Capture-MS Homo sapiens
67 MKS1 54903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 VPS13B  
Affinity Capture-MS Homo sapiens
69 OPALIN  
Affinity Capture-MS Homo sapiens
70 MOCOS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HDAC6 10013
Affinity Capture-MS Homo sapiens
72 TTC28 23331
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPAP1  
Affinity Capture-MS Homo sapiens
74 TRIM65 201292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 TUBB3 10381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 RHOT2 89941
Affinity Capture-MS Homo sapiens
77 S100A9 6280
Affinity Capture-MS Homo sapiens
78 DDX58 23586
Affinity Capture-RNA Homo sapiens
79 TUBB1 81027
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 ELMO2 63916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 DDX11 1663
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 Cdc37 12539
Affinity Capture-MS Mus musculus
83 ACACB 32
Affinity Capture-MS Homo sapiens
84 KRAS 3845
Proximity Label-MS Homo sapiens
85 WDR19 57728
Affinity Capture-MS Homo sapiens
86 RIF1  
Affinity Capture-MS Homo sapiens
87 ANKRD54  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 CDC5L 988
Affinity Capture-MS Homo sapiens
89 PIK3R3 8503
Affinity Capture-MS Homo sapiens
90 INTU  
Affinity Capture-MS Homo sapiens
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