Gene description for TRIM65
Gene name tripartite motif containing 65
Gene symbol TRIM65
Other names/aliases 4732463G12Rik
Species Homo sapiens
 Database cross references - TRIM65
ExoCarta ExoCarta_201292
Vesiclepedia VP_201292
Entrez Gene 201292
HGNC 27316
UniProt Q6PJ69  
 TRIM65 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for TRIM65
Molecular Function
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 IMP
    positive regulation of protein oligomerization GO:0032461 IEA
    positive regulation of interferon-alpha production GO:0032727 IEA
    positive regulation of interferon-beta production GO:0032728 IDA
    negative regulation of inflammatory response GO:0050728 IDA
    type I interferon-mediated signaling pathway GO:0060337 IEA
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    antiviral innate immune response GO:0140374 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified TRIM65 in exosomes
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRIM65
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK20  
Affinity Capture-MS Homo sapiens
2 VCAM1 7412
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
4 DHFR 1719
Affinity Capture-MS Homo sapiens
5 C12orf74  
Affinity Capture-MS Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 NPAS1  
Affinity Capture-MS Homo sapiens
9 NSUN5P1  
Affinity Capture-MS Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 PPP4R1L  
Affinity Capture-MS Homo sapiens
12 C9orf163  
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ARRDC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 KDELC1 79070
Affinity Capture-MS Homo sapiens
17 P4HA2 8974
Affinity Capture-MS Homo sapiens
18 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 UBE2Z 65264
Two-hybrid Homo sapiens
20 ISCA1  
Affinity Capture-MS Homo sapiens
21 CUL3 8452
Affinity Capture-MS Homo sapiens
22 UBE3A 7337
Two-hybrid Homo sapiens
23 MARCH7  
Two-hybrid Homo sapiens
24 MKRN3  
Two-hybrid Homo sapiens
25 PKD1L2  
Affinity Capture-MS Homo sapiens
26 PDDC1 347862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 BPNT1 10380
Affinity Capture-MS Homo sapiens
29 ANAPC11  
Two-hybrid Homo sapiens
30 CCNJL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SNX21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DNAJB3 414061
Affinity Capture-MS Homo sapiens
33 DUSP16  
Affinity Capture-MS Homo sapiens
34 TNRC6B  
Affinity Capture-MS Homo sapiens
35 ARHGAP35 2909
Co-localization Homo sapiens
36 UBE2D2 7322
Reconstituted Complex Homo sapiens
37 NOMO3 408050
Affinity Capture-MS Homo sapiens
38 ARHGAP25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TNRC6A 27327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
40 INF2 64423
Affinity Capture-MS Homo sapiens
41 LYPD4  
Affinity Capture-MS Homo sapiens
42 C16orf72 29035
Affinity Capture-MS Homo sapiens
43 ANKRD39  
Affinity Capture-MS Homo sapiens
44 C15orf41  
Affinity Capture-MS Homo sapiens
45 SRPK1 6732
Biochemical Activity Homo sapiens
46 OC90  
Affinity Capture-MS Homo sapiens
47 C19orf25  
Affinity Capture-MS Homo sapiens
48 ALDH3B1 221
Affinity Capture-MS Homo sapiens
49 SHARPIN  
Affinity Capture-MS Homo sapiens
50 HUWE1 10075
Affinity Capture-MS Homo sapiens
51 WDR5B  
Affinity Capture-MS Homo sapiens
52 NPRL2  
Affinity Capture-MS Homo sapiens
53 NUP210P1  
Affinity Capture-MS Homo sapiens
54 INSC  
Affinity Capture-MS Homo sapiens
55 GUSBP5  
Affinity Capture-MS Homo sapiens
56 AXIN1  
Affinity Capture-Western Homo sapiens
57 ALOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RBCK1  
Two-hybrid Homo sapiens
59 UBE2U  
Two-hybrid Homo sapiens
60 METTL21B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TRIM65 is involved
No pathways found





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