Gene description for VCAM1
Gene name vascular cell adhesion molecule 1
Gene symbol VCAM1
Other names/aliases CD106
INCAM-100
Species Homo sapiens
 Database cross references - VCAM1
ExoCarta ExoCarta_7412
Vesiclepedia VP_7412
Entrez Gene 7412
HGNC 12663
MIM 192225
UniProt P19320  
 VCAM1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for VCAM1
Molecular Function
    primary amine oxidase activity GO:0008131 IDA
    integrin binding GO:0005178 IMP
    cell adhesion molecule binding GO:0050839 IPI
Biological Process
    cell adhesion GO:0007155 IDA
    viral process GO:0016032 IEA
    chronic inflammatory response GO:0002544 IEA
    leukocyte cell-cell adhesion GO:0007159 IDA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    oxidation-reduction process GO:0055114 IDA
    response to ethanol GO:0045471 IEA
    aging GO:0007568 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    response to hypoxia GO:0001666 IEA
    heart development GO:0007507 IEA
    extracellular matrix organization GO:0030198 TAS
    membrane to membrane docking GO:0022614 IEP
    response to nicotine GO:0035094 IEA
    regulation of immune response GO:0050776 TAS
    response to nutrient GO:0007584 IEA
    acute inflammatory response GO:0002526 IEA
    response to zinc ion GO:0010043 IEA
    chorio-allantoic fusion GO:0060710 IEA
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEA
    cytokine-mediated signaling pathway GO:0019221 TAS
    cell chemotaxis GO:0060326 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    B cell differentiation GO:0030183 IC
    cell-matrix adhesion GO:0007160 IDA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IDA
    leukocyte tethering or rolling GO:0050901 IEP
    response to ionizing radiation GO:0010212 IEA
    amine metabolic process GO:0009308 IDA
    positive regulation of T cell proliferation GO:0042102 IDA
    response to lipopolysaccharide GO:0032496 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular space GO:0005615 IDA
    filopodium GO:0030175 IDA
    integral component of membrane GO:0016021 IEA
    microvillus GO:0005902 IDA
    cell surface GO:0009986 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    external side of plasma membrane GO:0009897 IDA
    extracellular exosome GO:0070062 IDA
    early endosome GO:0005769 IDA
    podosome GO:0002102 IDA
    apical part of cell GO:0045177 IDA
    alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex GO:0071065 IDA
    sarcolemma GO:0042383 IEA
 Experiment description of studies that identified VCAM1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for VCAM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Invivo Homo sapiens
2 EZR 7430
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 IL13 3596
Invitro Homo sapiens
4 CCL22 6367
Invitro Homo sapiens
5 MSN 4478
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 CCL17  
Invitro Homo sapiens
7 ITGAD  
Reconstituted Complex Homo sapiens
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