Gene description for HIP1R
Gene name huntingtin interacting protein 1 related
Gene symbol HIP1R
Other names/aliases HIP12
HIP3
ILWEQ
Species Homo sapiens
 Database cross references - HIP1R
ExoCarta ExoCarta_9026
Vesiclepedia VP_9026
Entrez Gene 9026
HGNC 18415
MIM 605613
UniProt O75146  
 HIP1R identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HIP1R
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    SH3 domain binding GO:0017124 IEA
    clathrin binding GO:0030276 IDA
    clathrin light chain binding GO:0032051 IBA
    phosphatidylinositol binding GO:0035091 IDA
    clathrin adaptor activity GO:0035615 IBA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IBA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    protein heterodimerization activity GO:0046982 IEA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    endocytosis GO:0006897 IBA
    receptor-mediated endocytosis GO:0006898 IMP
    actin filament organization GO:0007015 IBA
    regulation of endocytosis GO:0030100 IMP
    regulation of endocytosis GO:0030100 NAS
    negative regulation of actin filament polymerization GO:0030837 IMP
    regulation of actin cytoskeleton organization GO:0032956 IMP
    negative regulation of Arp2/3 complex-mediated actin nucleation GO:0034316 IEA
    regulation of apoptotic process GO:0042981 IBA
    negative regulation of apoptotic process GO:0043066 IEA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IBA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    clathrin coat assembly GO:0048268 IBA
    protein stabilization GO:0050821 IDA
    digestive system development GO:0055123 IEA
    regulation of gastric acid secretion GO:0060453 IEA
    intrinsic apoptotic signaling pathway GO:0097193 IGI
    postsynapse organization GO:0099173 IEA
    positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901030 IGI
    positive regulation of clathrin coat assembly GO:1905445 IEA
    regulation of clathrin-dependent endocytosis GO:2000369 NAS
    positive regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000588 IBA
    positive regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000588 IMP
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IEA
    postsynaptic density GO:0014069 ISS
    apical plasma membrane GO:0016324 IEA
    clathrin-coated vesicle GO:0030136 IBA
    clathrin-coated vesicle GO:0030136 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    cortical actin cytoskeleton GO:0030864 IBA
    ruffle membrane GO:0032587 IDA
    dendrite cytoplasm GO:0032839 ISS
    neuronal cell body GO:0043025 ISS
    dendritic spine GO:0043197 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    synaptic membrane GO:0097060 ISS
    postsynaptic endocytic zone GO:0098843 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified HIP1R in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HIP1R
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGCA  
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
5 GART 2618
Co-fractionation Homo sapiens
6 DIS3 22894
Co-fractionation Homo sapiens
7 TRAF4 9618
Co-fractionation Homo sapiens
8 CMTM8  
Affinity Capture-MS Homo sapiens
9 KLC3  
Affinity Capture-MS Homo sapiens
10 GAK 2580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GLS2 27165
Affinity Capture-MS Homo sapiens
12 TSG101 7251
Co-fractionation Homo sapiens
13 FPR1  
Affinity Capture-MS Homo sapiens
14 TYROBP  
Affinity Capture-MS Homo sapiens
15 MCCC1 56922
Co-fractionation Homo sapiens
16 Actb 11461
Affinity Capture-MS Mus musculus
17 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 FFAR1  
Affinity Capture-MS Homo sapiens
19 SOX2  
Affinity Capture-MS Homo sapiens
20 DIRAS3  
Proximity Label-MS Homo sapiens
21 SLC18A2  
Affinity Capture-MS Homo sapiens
22 IRF2BP1 26145
Co-fractionation Homo sapiens
23 B3GAT1  
Proximity Label-MS Homo sapiens
24 PCNT  
Proximity Label-MS Homo sapiens
25 NDRG1 10397
Co-fractionation Homo sapiens
26 HIP1 3092
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
28 EWSR1 2130
Co-fractionation Homo sapiens
29 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PPM1A 5494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TACSTD2 4070
Affinity Capture-MS Homo sapiens
32 ITGA4 3676
Affinity Capture-MS Homo sapiens
33 TATDN1 83940
Co-fractionation Homo sapiens
34 PHF11 51131
Affinity Capture-MS Homo sapiens
35 VCAM1 7412
Affinity Capture-MS Homo sapiens
36 GTSE1 51512
Affinity Capture-MS Homo sapiens
37 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 Sec24c  
Affinity Capture-MS Mus musculus
39 SARS 6301
Co-fractionation Homo sapiens
40 Lima1  
Affinity Capture-MS Mus musculus
41 LAMTOR1 55004
Proximity Label-MS Homo sapiens
42 PPM1B 5495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 C19orf38  
Affinity Capture-MS Homo sapiens
45 CEP41  
Co-fractionation Homo sapiens
46 PDGFRB 5159
Affinity Capture-MS Homo sapiens
47 TMEM150A  
Affinity Capture-MS Homo sapiens
48 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TUFM 7284
Co-fractionation Homo sapiens
50 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RPA2 6118
Proximity Label-MS Homo sapiens
52 MYH9 4627
Affinity Capture-MS Homo sapiens
53 VCP 7415
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 CRKL 1399
Co-fractionation Homo sapiens
56 GPR182  
Affinity Capture-MS Homo sapiens
57 AP1B1 162
Affinity Capture-MS Homo sapiens
58 SSUH2  
Affinity Capture-MS Homo sapiens
59 UPK1A 11045
Affinity Capture-MS Homo sapiens
60 TEX29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PICALM 8301
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
62 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SLC2A9  
Affinity Capture-MS Homo sapiens
64 LRFN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 LIMA1 51474
Affinity Capture-MS Homo sapiens
66 CD40 958
Affinity Capture-MS Homo sapiens
67 SFPQ 6421
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC15A1  
Affinity Capture-MS Homo sapiens
69 CYB5B 80777
Affinity Capture-MS Homo sapiens
70 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 RALBP1 10928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 LRRC25  
Affinity Capture-MS Homo sapiens
73 PTH2R  
Affinity Capture-MS Homo sapiens
74 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 MGARP  
Affinity Capture-MS Homo sapiens
76 MYO19  
Affinity Capture-MS Homo sapiens
77 AQP3  
Affinity Capture-MS Homo sapiens
78 SDC2 6383
Affinity Capture-MS Homo sapiens
79 FN1 2335
Affinity Capture-MS Homo sapiens
80 OCIAD1 54940
Affinity Capture-MS Homo sapiens
81 KIAA1467 57613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SYP  
Affinity Capture-MS Homo sapiens
83 UBE2D2 7322
Affinity Capture-MS Homo sapiens
84 MAP2K3 5606
Co-fractionation Homo sapiens
85 PSPC1 55269
Co-fractionation Homo sapiens
86 RPA3 6119
Proximity Label-MS Homo sapiens
87 SLC22A2 6582
Affinity Capture-MS Homo sapiens
88 TRIM27  
Two-hybrid Homo sapiens
89 NDC80 10403
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 RHOB 388
Proximity Label-MS Homo sapiens
92 CLTC 1213
Affinity Capture-MS Homo sapiens
93 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 STX17 55014
Affinity Capture-MS Homo sapiens
95 CDHR2 54825
Affinity Capture-MS Homo sapiens
96 GYPA  
Affinity Capture-MS Homo sapiens
97 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
98 VPS28 51160
Co-fractionation Homo sapiens
99 LDLRAD1  
Affinity Capture-MS Homo sapiens
100 MFSD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 Myo1c 17913
Affinity Capture-MS Mus musculus
102 BOP1 23246
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
103 CAPZA2 830
Affinity Capture-MS Homo sapiens
104 C10orf88  
Affinity Capture-MS Homo sapiens
105 BSND  
Affinity Capture-MS Homo sapiens
106 REPS1 85021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 C14orf166 51637
Co-fractionation Homo sapiens
109 SNW1 22938
Affinity Capture-MS Homo sapiens
110 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
111 RC3H1 149041
Co-fractionation Homo sapiens
112 SLC1A1 6505
Affinity Capture-MS Homo sapiens
113 WARS 7453
Co-fractionation Homo sapiens
114 CCR1  
Affinity Capture-MS Homo sapiens
115 CDC5L 988
Affinity Capture-MS Homo sapiens
116 MTMR6 9107
Co-fractionation Homo sapiens
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