Gene description for SLC1A1
Gene name solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Gene symbol SLC1A1
Other names/aliases DCBXA
EAAC1
EAAT3
SCZD18
Species Homo sapiens
 Database cross references - SLC1A1
ExoCarta ExoCarta_6505
Vesiclepedia VP_6505
Entrez Gene 6505
HGNC 10939
MIM 133550
UniProt P43005  
 SLC1A1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for SLC1A1
Molecular Function
    monoatomic anion channel activity GO:0005253 IEA
    L-glutamate transmembrane transporter activity GO:0005313 IBA
    L-glutamate transmembrane transporter activity GO:0005313 IDA
    high-affinity L-glutamate transmembrane transporter activity GO:0005314 IDA
    high-affinity L-glutamate transmembrane transporter activity GO:0005314 TAS
    protein binding GO:0005515 IPI
    chloride transmembrane transporter activity GO:0015108 IMP
    L-aspartate transmembrane transporter activity GO:0015183 IDA
    glutamate:sodium symporter activity GO:0015501 IBA
    glutamate:sodium symporter activity GO:0015501 IDA
    glutamate binding GO:0016595 IEA
    cysteine transmembrane transporter activity GO:0033229 IBA
    cysteine transmembrane transporter activity GO:0033229 IDA
    identical protein binding GO:0042802 IEA
    metal ion binding GO:0046872 IEA
    D-aspartate transmembrane transporter activity GO:0140010 IDA
Biological Process
    behavioral fear response GO:0001662 IEA
    response to amphetamine GO:0001975 IEA
    glutathione biosynthetic process GO:0006750 IEA
    superoxide metabolic process GO:0006801 IEA
    monoatomic ion transport GO:0006811 TAS
    neurotransmitter transport GO:0006836 TAS
    intracellular zinc ion homeostasis GO:0006882 IEA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 IEA
    glutamate receptor signaling pathway GO:0007215 IEA
    chemical synaptic transmission GO:0007268 TAS
    brain development GO:0007420 IEA
    memory GO:0007613 IEA
    grooming behavior GO:0007625 IEA
    locomotory behavior GO:0007626 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of heart rate GO:0010460 IEA
    gene expression GO:0010467 IEA
    retina layer formation GO:0010842 IEA
    L-glutamate transmembrane transport GO:0015813 IBA
    L-glutamate transmembrane transport GO:0015813 IDA
    cytokine-mediated signaling pathway GO:0019221 IEA
    neurogenesis GO:0022008 IEA
    adult behavior GO:0030534 IEA
    cellular response to oxidative stress GO:0034599 IEA
    maintenance of blood-brain barrier GO:0035633 ISS
    response to decreased oxygen levels GO:0036293 IEA
    dopamine metabolic process GO:0042417 IEA
    cysteine transport GO:0042883 IDA
    response to morphine GO:0043278 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    blood vessel morphogenesis GO:0048514 ISS
    response to axon injury GO:0048678 IEA
    synapse organization GO:0050808 IEA
    L-glutamate import GO:0051938 IDA
    righting reflex GO:0060013 IEA
    heart contraction GO:0060047 IEA
    long-term synaptic potentiation GO:0060291 IEA
    motor behavior GO:0061744 IEA
    transepithelial transport GO:0070633 ISS
    D-aspartate transmembrane transport GO:0070777 IDA
    L-aspartate transmembrane transport GO:0070778 IDA
    L-aspartate transmembrane transport GO:0070778 ISS
    D-aspartate import across plasma membrane GO:0070779 IDA
    D-aspartate import across plasma membrane GO:0070779 IMP
    cellular response to ammonium ion GO:0071242 IEA
    cellular response to mercury ion GO:0071288 IEA
    cellular response to cocaine GO:0071314 IEA
    cellular response to organic cyclic compound GO:0071407 IDA
    zinc ion transmembrane transport GO:0071577 IEA
    response to anesthetic GO:0072347 IEA
    regulation of protein targeting to membrane GO:0090313 IEA
    intracellular glutamate homeostasis GO:0090461 IEA
    motor neuron apoptotic process GO:0097049 IEA
    L-glutamate import across plasma membrane GO:0098712 IDA
    L-glutamate import across plasma membrane GO:0098712 IMP
    neurotransmitter receptor transport to plasma membrane GO:0098877 IEA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IEA
    L-aspartate import across plasma membrane GO:0140009 IDA
    L-aspartate import across plasma membrane GO:0140009 IMP
    transport across blood-brain barrier GO:0150104 ISS
    transport across blood-brain barrier GO:0150104 NAS
    chloride transmembrane transport GO:1902476 IMP
    cysteine transmembrane transport GO:1903712 IEA
    cellular response to bisphenol A GO:1903926 IEA
    conditioned place preference GO:1990708 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IEA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISS
    apical plasma membrane GO:0016324 IDA
    axon GO:0030424 ISS
    dendrite GO:0030425 ISS
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IEA
    asymmetric synapse GO:0032279 ISS
    neuronal cell body GO:0043025 ISS
    synaptic cleft GO:0043083 ISS
    dendritic spine GO:0043197 ISS
    dendritic shaft GO:0043198 ISS
    perikaryon GO:0043204 ISS
    axon terminus GO:0043679 ISS
    membrane raft GO:0045121 IEA
    recycling endosome membrane GO:0055038 IEA
    extracellular exosome GO:0070062 HDA
    cell periphery GO:0071944 ISS
    glial cell projection GO:0097386 ISS
    apical dendrite GO:0097440 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    presynapse GO:0098793 ISS
    perisynaptic space GO:0099544 ISS
    distal dendrite GO:0150002 ISS
    proximal dendrite GO:1990635 ISS
 Experiment description of studies that identified SLC1A1 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 13
MISEV standards
IEM
Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
5
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC1A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DPY19L1 23333
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 KDELR3 11015
Affinity Capture-MS Homo sapiens
4 AXL 558
Affinity Capture-MS Homo sapiens
5 KRT73 319101
Affinity Capture-MS Homo sapiens
6 SLC31A2  
Affinity Capture-MS Homo sapiens
7 SNX3 8724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLC35A1 10559
Two-hybrid Homo sapiens
9 TMBIM6 7009
Affinity Capture-MS Homo sapiens
10 TM9SF1 10548
Affinity Capture-MS Homo sapiens
11 CD3D 915
Affinity Capture-MS Homo sapiens
12 CMTM8  
Affinity Capture-MS Homo sapiens
13 ORMDL3 94103
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ARL6IP5 10550
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 PTPMT1 114971
Affinity Capture-MS Homo sapiens
16 VMP1 81671
Affinity Capture-MS Homo sapiens
17 SURF4 6836
Affinity Capture-MS Homo sapiens
18 TMEM9B 56674
Affinity Capture-MS Homo sapiens
19 KLRB1  
Affinity Capture-MS Homo sapiens
20 TMEM72  
Affinity Capture-MS Homo sapiens
21 DERL1 79139
Affinity Capture-MS Homo sapiens
22 CMTM6 54918
Affinity Capture-MS Homo sapiens
23 FFAR1  
Affinity Capture-MS Homo sapiens
24 CPT1A 1374
Affinity Capture-MS Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 TMEM55B 90809
Affinity Capture-MS Homo sapiens
27 SPG7  
Affinity Capture-MS Homo sapiens
28 TOMM22 56993
Affinity Capture-MS Homo sapiens
29 GOLGA7 51125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TMEM179B 374395
Two-hybrid Homo sapiens
31 PTDSS2 81490
Affinity Capture-MS Homo sapiens
32 SLC20A1 6574
Affinity Capture-MS Homo sapiens
33 RNF5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 LPGAT1 9926
Affinity Capture-MS Homo sapiens
35 TMEM41B 440026
Affinity Capture-MS Homo sapiens
36 HLA-B 3106
Affinity Capture-MS Homo sapiens
37 NUMBL 9253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TIMMDC1  
Affinity Capture-MS Homo sapiens
39 SPAG9 9043
Affinity Capture-MS Homo sapiens
40 SFXN3 81855
Affinity Capture-MS Homo sapiens
41 TM9SF4 9777
Affinity Capture-MS Homo sapiens
42 TMEM165 55858
Affinity Capture-MS Homo sapiens
43 YIPF3 25844
Affinity Capture-MS Homo sapiens
44 EWSR1 2130
Two-hybrid Homo sapiens
45 FAM210A  
Affinity Capture-MS Homo sapiens
46 CST9L  
Affinity Capture-MS Homo sapiens
47 SLC25A19  
Affinity Capture-MS Homo sapiens
48 SFXN2  
Affinity Capture-MS Homo sapiens
49 SORT1 6272
Affinity Capture-MS Homo sapiens
50 ACSL1 2180
Affinity Capture-MS Homo sapiens
51 MPC2 25874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 GPX8 493869
Affinity Capture-MS Homo sapiens
53 FGFR1 2260
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SLC25A33  
Affinity Capture-MS Homo sapiens
55 AGPAT2  
Affinity Capture-MS Homo sapiens
56 NDFIP1 80762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PXMP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 EXTL3 2137
Affinity Capture-MS Homo sapiens
59 AUP1 550
Affinity Capture-MS Homo sapiens
60 SLC1A3 6507
Affinity Capture-MS Homo sapiens
61 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
62 FCER1G 2207
Two-hybrid Homo sapiens
63 POMGNT1 55624
Affinity Capture-MS Homo sapiens
64 SEC61A2 55176
Affinity Capture-MS Homo sapiens
65 COX15 1355
Affinity Capture-MS Homo sapiens
66 TMEM126A 84233
Affinity Capture-MS Homo sapiens
67 IFITM3 10410
Affinity Capture-MS Homo sapiens
68 CST6  
Affinity Capture-MS Homo sapiens
69 SBSN 374897
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 P2RY8  
Affinity Capture-MS Homo sapiens
71 TUBA1A 7846
Affinity Capture-MS Homo sapiens
72 MICU2 221154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 VSIG4  
Affinity Capture-MS Homo sapiens
74 RELL2  
Affinity Capture-MS Homo sapiens
75 CHPT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ACVR1B 91
Affinity Capture-MS Homo sapiens
77 UBXN8  
Affinity Capture-MS Homo sapiens
78 NUMB 8650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ALDH3B1 221
Affinity Capture-MS Homo sapiens
80 LRBA 987
Affinity Capture-MS Homo sapiens
81 SLC25A17 10478
Affinity Capture-MS Homo sapiens
82 CHCHD6 84303
Affinity Capture-MS Homo sapiens
83 ACSL4 2182
Affinity Capture-MS Homo sapiens
84 TMEM70  
Affinity Capture-MS Homo sapiens
85 JAK1 3716
Affinity Capture-MS Homo sapiens
86 C5orf15 56951
Affinity Capture-MS Homo sapiens
87 UPK2 7379
Affinity Capture-MS Homo sapiens
88 OXA1L  
Affinity Capture-MS Homo sapiens
89 FNDC3B 64778
Affinity Capture-MS Homo sapiens
90 CD320 51293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 SLC25A25  
Affinity Capture-MS Homo sapiens
92 RARS2  
Affinity Capture-MS Homo sapiens
93 PSEN1 5663
Affinity Capture-MS Homo sapiens
94 CXCR4 7852
Affinity Capture-MS Homo sapiens
95 VAPB 9217
Affinity Capture-MS Homo sapiens
96 RAB7A 7879
Proximity Label-MS Homo sapiens
97 YIPF6 286451
Affinity Capture-MS Homo sapiens
98 DHCR24 1718
Affinity Capture-MS Homo sapiens
99 TVP23C  
Affinity Capture-MS Homo sapiens
100 POLD3  
Affinity Capture-MS Homo sapiens
101 SLC25A22  
Affinity Capture-MS Homo sapiens
102 ERGIC3 51614
Two-hybrid Homo sapiens
103 RAB29 8934
Affinity Capture-MS Homo sapiens
104 DAD1 1603
Affinity Capture-MS Homo sapiens
105 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
106 NIPAL1  
Affinity Capture-MS Homo sapiens
107 LGALS9 3965
Affinity Capture-MS Homo sapiens
108 AQP3  
Affinity Capture-MS Homo sapiens
109 ARL8A 127829
Affinity Capture-MS Homo sapiens
110 HIP1R 9026
Affinity Capture-MS Homo sapiens
111 TMEM169  
Affinity Capture-MS Homo sapiens
112 FAM209A 200232
Two-hybrid Homo sapiens
113 ACSL3 2181
Affinity Capture-MS Homo sapiens
114 SNX17 9784
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 TMCO1 54499
Affinity Capture-MS Homo sapiens
116 DHRS7B 25979
Affinity Capture-MS Homo sapiens
117 TMEM246 84302
Affinity Capture-MS Homo sapiens
118 ASPH 444
Affinity Capture-MS Homo sapiens
119 VRK2 7444
Affinity Capture-MS Homo sapiens
120 YIPF5 81555
Affinity Capture-MS Homo sapiens
121 ADCK4 79934
Affinity Capture-MS Homo sapiens
122 NRN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
124 KIAA0368 23392
Affinity Capture-MS Homo sapiens
125 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 NTSR1  
Affinity Capture-MS Homo sapiens
127 TMEM209 84928
Affinity Capture-MS Homo sapiens
128 PEX3 8504
Affinity Capture-MS Homo sapiens
129 STX17 55014
Affinity Capture-MS Homo sapiens
130 ADGRE5 976
Affinity Capture-MS Homo sapiens
131 TSPAN10 83882
Affinity Capture-MS Homo sapiens
132 YIF1B 90522
Affinity Capture-MS Homo sapiens
133 C1GALT1 56913
Two-hybrid Homo sapiens
134 GPR89A  
Affinity Capture-MS Homo sapiens
135 TMEM9 252839
Affinity Capture-MS Homo sapiens
136 SYNPR  
Two-hybrid Homo sapiens
137 KDELR1 10945
Affinity Capture-MS Homo sapiens
138 DNAJC19 131118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 OPALIN  
Affinity Capture-MS Homo sapiens
140 LDLRAD1  
Two-hybrid Homo sapiens
141 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 EPHA4 2043
Affinity Capture-MS Homo sapiens
143 C7orf55  
Affinity Capture-MS Homo sapiens
144 RIPK3 11035
Affinity Capture-MS Homo sapiens
145 SLC25A40  
Affinity Capture-MS Homo sapiens
146 PPOX 5498
Affinity Capture-MS Homo sapiens
147 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 ELOVL1 64834
Affinity Capture-MS Homo sapiens
149 STARD3 10948
Affinity Capture-MS Homo sapiens
150 GZMH  
Affinity Capture-MS Homo sapiens
151 VAMP7 6845
Affinity Capture-MS Homo sapiens
152 RAB8A 4218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 HACD2 201562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
154 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 LAGE3  
Affinity Capture-MS Homo sapiens
156 SLC25A4 291
Affinity Capture-MS Homo sapiens
157 PRKCA 5578
Affinity Capture-Western Homo sapiens
158 RAB11A 8766
Proximity Label-MS Homo sapiens
159 SLC1A4 6509
Affinity Capture-MS Homo sapiens
160 AGPAT6 137964
Affinity Capture-MS Homo sapiens
161 CXCL16 58191
Affinity Capture-MS Homo sapiens
162 CHSY1 22856
Affinity Capture-MS Homo sapiens
163 APOOL 139322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 CC2D1A 54862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
165 S1PR4  
Affinity Capture-MS Homo sapiens
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